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This study presents an efficient isolation of human lung carcinoma cell line (A549) platform by utilizing the proposal a serpentine microchannel with added cavities (SMAC) platform. The magnetic micro-bead isolation performance in proposed serpentine channel was simulated using COMSOL Multiphysics. The experimental procedures of aptamer molecular conjugation on magnetic microbeads affined to A549 cell line were also reported. The experimental results indicated that at the flow rate of 5 µL/s, the capture rate of human lung carcinoma cell reached above 70% in less than 15 min, whereas non-target cell capture rate was around 1.5%. The proposed platform shows its potential of high selectivity, portable, and simple-to-operate, which are suitable considerations for developing point-of-care applications in various biological and clinical purposes.

This paper presents a dielectrophoresis (DEP) enrichment microfluidic platform with built-in antibody-based capacitive sensor for tumor rare cells detection. We take the advantages of the effective DEP actuation, the high selectivity property of antibody for rare cell immobilization, and the high sensitivity of differential capacitive sensing for quantitatively reading out, to produce advanced platform, toward single tumor cell detection for the rapid laboratory tests of cancers diagnoses and other metabolic diseases applications.

In this paper, we present a coplanar differential capacitively coupled contactless conductivity detection (CD-C4D) structure for the detection of tiny objects with dimension in micro scale. The differential capacitance changed due to the occurrence of an object in micro scale in is detected and used to recognize the presence as well as properties (i.e., electrical property and moving speed) of the object in fluidic flow. The experimental results and discussion in this paper can be generalized to any living cell or particle detection applications.