1. Discovery of Amphoteric Fingerprints of Amino Acids With Field-Effect Transistors
- Author
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Naveen Kumar, Rakshita Pritam Singh Dhar, Janwa El Maiss, Vihar Georgiev, and Cesar Pascual Garcia
- Subjects
Amino acids ,fingerprints ,Gouy-Chapman-Stern model ,ISFET ,polypeptides ,site-binding model ,Electrical engineering. Electronics. Nuclear engineering ,TK1-9971 - Abstract
When working with a few molecules, the existing protein sequencing technologies lack the single-site amino acid (AA) precision required to detect abnormalities that escape genome analysis. We offer a new method for identifying AAs and short polypeptides using the signal from the surface potential and capacitance obtained from Field Effect Transistor (FET) sensors. Using a combination of the Site-binding and Gouy-Chapman-Stern (GCS) models, we found the signatures of every single AA and polypeptides. These fingerprints are based on orthogonal features such as the proton dissociation constants of each AA’s charging sites, the dielectric constant, and the effective length.
- Published
- 2024
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