Search

The paper aims at presenting the acute toxic effect of water-accommodated fractions（WAFS） of No.0 diesel oil on HOX1 gene of Hemicentrotus pulcherrimus by PCR-SSCP. The experiment was carried out with three concentrations(2mg/L, 5mg/L, 10mg/l). The results indicated that with the increase of exposure concentration and time, the probability of mutation increased. In the four primers, single base mutation took place in coding region of the 184 in primer N-2, with the conversion of T-C, where amino acid changed from serine into proline.

The Marine Oil Spill Response Information System, which serves as a decision support system designed to effectively response to the marine oil spill accidents, has been developed through the integration of a series of numerical models with the Geographic Information System (GIS). In the system, the three dimensional hydrodynamic model, oil spill trajectory and fate models are embedded in GIS. The oil spill response and environmental databases are also established to provide decision support to the emergency teams. Finally the function of the system is illustrated through a case study of an oil spill accident which happened at the Pearl River Estuary of Guangdong Province of China in December 2004. The simulation gave a reasonable estimation of spilled oil trajectory with limited environmental input data. The accurate prediction of the behavior of the spilled oil is crucial to the successful recovery operation and protection of marine environment.

The paper aims at present the acute toxic effect of water-accommodated fraction (WAFs) of No.0 diesel oil on embryos and larvae development of sea urchin, Hemicentrotus pulcherrimus by adopting embryonic development technology. The experiment was carried out with six concentrations. The results indicate that the water-accommodated fractions have evident toxic effect on two - arm and four - arm larvae. Compared with the control group, the larvae in the oil groups showed shorter body lengths and higher deformity and mortality rate by the increasing concentrations. The 48 h, 72 h EC50 is 3.39, 1.87 mg/l respectively.