1. An Innovative Strategy for Reciprocal Distant Hybridization between Spartina alterniflora and Rice.
- Author
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Qikang CHEN, Bo CHEN, Huilan LU, Hui ZHOU, and Zhiping MEI
- Subjects
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SPARTINA alterniflora , *SALT-tolerant crops , *RICE yields , *HYBRID rice , *POLLINATION , *SPECIES hybridization , *PLANT germplasm - Abstract
[Objective] This study aimed to explore germ plasm resources of salt tolerant Spartina alterniflora and high yield rice, and develop an innovative strategy for distant hybridization between S. alterniflora and rice. [Method] Crossing (rice ♀): hot water emasculation and glume-cutting pollination method, heat emasculation and pollen-suction pollination method; reciprocal crossing (S. alterniflora ♀): glume-cutting emasculation and pollination method, continuous emasculation-pollination method, hot water emasculation and continuous emasculation-pollination method. [Result] In crossing groups (rice ♀ x S. alterniflora ♂), compared with hot water emasculation and glume-cutting pollination treatment, the number of seed-bearing combinations, seed number per spike and seedling emergence rate in heat emasculation and pollen-suction pollination treatment were improved by 34.09%, 121.21% and 60.07%, respectively; hybridization efficiency was improved by 6-7 times. In reciprocal crossing groups (S. alterniflora ♀ x rice ♂), compared with glume-cutting emasculation and pollination treatment, the number of seed-bearing combinations and seed number per spike in continuous emasculation-pollination treatment were improved by 3.14 and 4.21 times, respectively; seedling emergence rate was improved by 68.47%; hybridization efficiency was improved by 7-8 times. [Conclusion] Heat emasculation and pollen-suction pollination method is suitable for hybridization between rice ♀ x S. alterniflora ♂ ; continuous emasculation-pollination method is suitable for hybridization between S. alterniflora ♀ x rice ♂. [ABSTRACT FROM AUTHOR]
- Published
- 2015