1. Mass Spectrometry-Based Quantitative O-GlcNAcomic Analysis.
- Author
-
Ma J and Hart GW
- Subjects
- Acetylglucosamine chemistry, Glycosylation, Humans, Phosphorylation, Protein Processing, Post-Translational, Mass Spectrometry methods
- Abstract
The dynamic co- and post-translational modification (PTM) of proteins, O-linked β-D-N-acetylglucosamine modification (O-GlcNAcylation) of serine/threonine residues is critical in many cellular processes, contributing to multiple physiological and pathological events. The term "O-GlcNAcome" refers to not only the complete set of proteins that undergo O-GlcNAcylation but also the O-GlcNAc status at individual residues, as well as the dynamics of O-GlcNAcylation in response to various stimuli. O-GlcNAcomic analyses have been a challenge for many years. In this chapter, we describe a recently developed approach for the identification and quantification of O-GlcNAc proteins/peptides from complex samples.
- Published
- 2016
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