1. Construction of Gene-Targeting Vectors by Recombineering
- Author
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Song-Choon, Lee, Wei, Wang, and Pentao, Liu
- Subjects
Mice, Knockout ,Recombination, Genetic ,Chromosomes, Artificial, Bacterial ,Mice ,Lac Operon ,Gene Targeting ,Genetic Vectors ,Animals ,Genetic Engineering ,Promoter Regions, Genetic ,Polymerase Chain Reaction ,Article ,Plasmids - Abstract
Recombineering is a technology that utilizes the efficient homologous recombination functions encoded by gamma phage to manipulate DNA in Escherichia coli. Construction of knockout vectors has been greatly facilitated by recombineering as it allows one to choose any genomic region to manipulate. We describe here an efficient recombineering-based protocol for making mouse conditional knockout targeting vectors.
- Published
- 2009
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