1. Characterization of Schistosoma mansoni Dihydrofolate Reductase (DHFR).
- Author
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Serrão VHB, Scortecci JF, and D'Muniz Pereira H
- Subjects
- Animals, Calorimetry, Crystallography, X-Ray, Enzyme Assays, Folic Acid analogs & derivatives, Freezing, Recombinant Proteins isolation & purification, Recombinant Proteins metabolism, Synchrotrons, Tetrahydrofolate Dehydrogenase chemistry, Tetrahydrofolate Dehydrogenase genetics, Tetrahydrofolate Dehydrogenase isolation & purification, Schistosoma mansoni enzymology, Tetrahydrofolate Dehydrogenase metabolism
- Abstract
Dihydrofolate reductase (DHFR) is an essential enzyme for nucleotide metabolism used to obtain energy and structural nucleic acids. Schistosoma mansoni has all the pathways for pyrimidine biosynthesis, which include the thymidylate cycle and, consequentially, the DHFR enzyme. Here, we describe the characterization of Schistosoma mansoni DHFR (SmDHFR) using isothermal titration calorimetry for the enzymatic activity and thermodynamic determination, also the folate analogs inhibition. Moreover, X-ray crystallography was used to determine the enzyme atomic model at 1.95 Å.
- Published
- 2020
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