1. Anchor Away: A System for Fast Inhibition of Proteins in Drosophila.
- Author
-
Sanchez Bosch P
- Subjects
- Animals, Mammals metabolism, Tacrolimus Binding Protein 1A metabolism, Tacrolimus Binding Proteins, Drosophila metabolism, Sirolimus pharmacology
- Abstract
Anchor away is a sequestering method designed to acutely and timely abrogate the function of a protein of interest by anchoring to a cell compartment different from its target. This method induces the binding of the target protein to the anchor by either the addition of rapamycin to Drosophila food or cell media. Rapamycin mediates the formation of a ternary complex between the anchor, which is tagged with the FK506-binding protein (FKBP12), and the target protein fused with the FKB12 rapamycin-binding (FRB) domain of mammalian target of rapamycin (mTOR). The rapamycin-bound target protein stays sequestered away from its compartment, where it cannot perform its biological function., (© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2022
- Full Text
- View/download PDF