1. Race Typing of Puccinia striiformis on Wheat.
- Author
-
Hovmøller MS, Rodriguez-Algaba J, Thach T, and Sørensen CK
- Subjects
- Basidiomycota pathogenicity, Basidiomycota physiology, Disease Resistance, Genotype, Genotyping Techniques methods, Host-Pathogen Interactions, Plant Diseases genetics, Seedlings genetics, Triticum genetics, Virulence, Basidiomycota genetics, Plant Diseases microbiology, Seedlings microbiology, Triticum microbiology
- Abstract
A procedure for virulence phenotyping of isolates of yellow (stripe) rust using spray inoculation of wheat seedlings by spores suspended in an engineered fluid, Novec™ 7100, is presented. Differential sets consisting of near-isogenic Avocet lines, selected lines from the "World" and "European" sets, and additional varieties showing race-specificity facilitate a robust assessment of race, irrespectively of geographical and evolutionary origin of isolates. A simple procedure for purification of samples consisting of multiple races is also presented.
- Published
- 2017
- Full Text
- View/download PDF