1. Dissection and Preparation of Human Primary Fetal Ganglionic Eminence Tissue for Research and Clinical Applications.
- Author
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Roberton VH, Rosser AE, McGorrian AM, and Precious SV
- Subjects
- Corpus Striatum cytology, Dissection instrumentation, Donor Selection legislation & jurisprudence, Donor Selection methods, Fetus, Humans, Neurons, Primary Cell Culture instrumentation, Tissue and Organ Procurement legislation & jurisprudence, Tissue and Organ Procurement methods, Corpus Striatum pathology, Dissection methods, Huntington Disease pathology, Primary Cell Culture methods
- Abstract
Here, we describe detailed dissection and enzymatic dissociation protocols for the ganglionic eminences from the developing human brain to generate viable quasi-single cell suspensions for subsequent use in transplantation or cell culture. These reliable and reproducible protocols can provide tissue for use in the study of the developing human brain, as well as for the preparation of donor cells for transplantation in Huntington's disease (HD). For use in the clinic as a therapy for HD, the translation of these protocols from the research laboratory to the GMP suite is described, including modification to reagents used and appropriate monitoring and tissue release criteria.
- Published
- 2018
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