1. Kinetic Detection of Apoptosis Events Via Caspase 3/7 Activation in a Tumor-Immune Microenvironment on a Chip.
- Author
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Bertani FR, Moghaddam FD, Panella C, Giannitelli SM, Peluzzi V, Gerardino A, Rainer A, Roscilli G, De Ninno A, and Businaro L
- Subjects
- Animals, Humans, Mice, Caspase 3, Microfluidics methods, Apoptosis, Lab-On-A-Chip Devices, Tumor Microenvironment, Neoplasms pathology
- Abstract
The development of advanced biological models like microphysiological systems, able to rebuild the complexity of the physiological and/or pathological environments at a single-cell detail level in an in-vivo-like approach, is proving to be a promising tool to understand the mechanisms of interactions between different cell populations and main features of several diseases. In this frame, the tumor-immune microenvironment on a chip represents a powerful tool to profile key aspects of cancer progression, immune activation, and response to therapy in several immuno-oncology applications. In the present chapter, we provide a protocol to identify and characterize the time evolution of apoptosis by time-lapse fluorescence and confocal imaging in a 3D microfluidic coculture murine model including cancer and spleen cells., (© 2024. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2024
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