1. Super-Resolution Imaging of Mitotic Spindle Microtubules Using STED Microscopy.
- Author
-
Koprivec I, Štimac V, and Tolić IM
- Subjects
- Humans, Mitosis, Image Processing, Computer-Assisted methods, HeLa Cells, Microtubules metabolism, Microtubules ultrastructure, Spindle Apparatus metabolism, Microscopy, Fluorescence methods, Kinetochores metabolism
- Abstract
Stimulated emission depletion (STED) microscopy is a powerful super-resolution imaging technique that only recently entered the field of mitosis, where it proved to be invaluable for studying various microtubule classes, kinetochore-microtubule attachments and chromosome segregation errors. Here, we describe immunofluorescence combined with STED microscopy as a method for analyzing microtubules and kinetochore-microtubule attachments in human mitotic spindles. We also describe live-cell STED microscopy as a method for single-plane short-term imaging of transient processes in crowded spindle areas. Finally, we outline image analysis approaches for the quantitative assessment of microtubule bundles within the spindle., (© 2025. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.)
- Published
- 2025
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