Search

Your search keyword '"Geysens, Steven"' showing total 3 results
Start Over Author "Geysens, Steven" Publisher humana press
3 results on '"Geysens, Steven"'

3. Modification of the N-glycosylation pathway to produce homogeneous, human-like glycans using GlycoSwitch plasmids.

Author

Vervecken W, Callewaert N, Kaigorodov V, Geysens S, and Contreras R

Subjects
Endoplasmic Reticulum enzymology, Glycosylation, Golgi Apparatus enzymology, Humans, Mannosidases metabolism, Mannosyltransferases genetics, N-Acetylglucosaminyltransferases metabolism, Pichia enzymology, Plasmids genetics, Polysaccharides biosynthesis
Abstract

Glycosylation is an important issue in heterologous protein production for therapeutic applications. Glycoproteins produced in Pichia pastoris contain high mannose glycan structures that can hamper downstream processing, might be immunogenic, and cause rapid clearance from the circulation. This chapter describes a method that helps solving these glycosylation-related problems by inactivation of OCH1, overexpression of an HDEL-tagged mannosidase, and overexpression of a Kre2/GlcNAc-transferase I chimeric enzyme. Different plasmids are described as well as glycan analysis methods.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results