1. Massively Parallel Signature Sequencing
- Author
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Mahendra S. Rao, Eric Vermaas, Takumi Miura, Christian D. Haudenschild, Roger Walker, Daixing Zhou, Irina Khrebtukova, Keith Moon, Shannon Decola, and Thomas J. Vasicek
- Subjects
Gene expression profiling ,Transcriptome ,Massive parallel sequencing ,Computer science ,Computational biology ,Throughput (business) ,Genome ,Massively parallel signature sequencing ,Sequence (medicine) - Abstract
Massively parallel signature sequencing is an ultra-high throughput sequencing technology. It can simultaneously sequence millions of sequence tags, and, therefore, is ideal for whole genome analysis. When applied to expression profiling, it reveals almost every transcript in the sample and provides its accurate expression level. This chapter describes the technology and its application in establishing stem cell transcriptome databases.
- Published
- 2006
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