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Start Over Search Limiters Academic (Peer-Reviewed) Journals Publisher hospital clin barcelona, spain
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1. Diabetes as a case study of chronic disease management with a personalized approach: The role of a structured feedback loop

Author

Ceriello, Antonio, Barkai, Laszlo, Sandahl Christiansen, Jens, Czupryniak, Leszek, Gomis, Ramon, Harno, Kari, Kulzer, Bernhard, Ludvigsson, Johnny, Nemethyova, Zuzana, Owens, David, Schnell, Oliver, Tankova, Tsvetalina, Taskinen, Marja-Riitta, Verges, Bruno, Weitgasser, Raimund, Wens, Johan, Ceriello, Antonio, Barkai, Laszlo, Sandahl Christiansen, Jens, Czupryniak, Leszek, Gomis, Ramon, Harno, Kari, Kulzer, Bernhard, Ludvigsson, Johnny, Nemethyova, Zuzana, Owens, David, Schnell, Oliver, Tankova, Tsvetalina, Taskinen, Marja-Riitta, Verges, Bruno, Weitgasser, Raimund, and Wens, Johan

Abstract

As non-communicable or chronic diseases are a growing threat to human health and economic growth, political stakeholders are aiming to identify options for improved response to the challenges of prevention and management of non-communicable diseases. This paper is intended to contribute ideas on personalized chronic disease management which are based on experience with one major chronic disease, namely diabetes mellitus. less thanbrgreater than less thanbrgreater thanDiabetes provides a pertinent case of chronic disease management with a particular focus on patient self-management. Despite advances in diabetes therapy, many people with diabetes still fail to achieve treatment targets thus remaining at risk of complications. Personalizing the management of diabetes according to the patients individual profile can help in improving therapy adherence and treatment outcomes. This paper suggests using a six-step cycle for personalized diabetes (self-)management and collaborative use of structured blood glucose data. E-health solutions can be used to improve process efficiencies and allow remote access. Decision support tools and algorithms can help doctors in making therapeutic decisions based on individual patient profiles. Available evidence about the effectiveness of the cycles constituting elements justifies expectations that the diabetes management cycle as a whole can generate medical and economic benefit.

Published
2012
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2. Advancing psychotherapy and evidence-based psychological interventions

Author

Emmelkamp, Paul M. G., David, Daniel, Beckers, Tom, Muris, Peter, Cuijpers, Pim, Lutz, Wolfgang, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Berking, Matthias, Berger, Thomas, Botella, Christina, Carlbring, Per, Colom, Francesc, Essau, Cecilia, Hermans, Dirk, Hofmann, Stefan G., Knappe, Susanne, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Van der Oord, Saskia, Vervliet, Bram, Emmelkamp, Paul M. G., David, Daniel, Beckers, Tom, Muris, Peter, Cuijpers, Pim, Lutz, Wolfgang, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Berking, Matthias, Berger, Thomas, Botella, Christina, Carlbring, Per, Colom, Francesc, Essau, Cecilia, Hermans, Dirk, Hofmann, Stefan G., Knappe, Susanne, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Van der Oord, Saskia, and Vervliet, Bram

Abstract

Psychological models of mental disorders guide research into psychological and environmental factors that elicit and maintain mental disorders as well as interventions to reduce them. This paper addresses four areas. (1) Psychological models of mental disorders have become increasingly transdiagnostic, focusing on core cognitive endophenotypes of psychopathology from an integrative cognitive psychology perspective rather than offering explanations for unitary mental disorders. It is argued that psychological interventions for mental disorders will increasingly target specific cognitive dysfunctions rather than symptom-based mental disorders as a result. (2) Psychotherapy research still lacks a comprehensive conceptual framework that brings together the wide variety of findings, models and perspectives. Analysing the state-of-the-art in psychotherapy treatment research, component analyses aiming at an optimal identification of core ingredients and the mechanisms of change is highlighted as the core need towards improved efficacy and effectiveness of psychotherapy, and improved translation to routine care. (3) In order to provide more effective psychological interventions to children and adolescents, there is a need to develop new and/or improved psychotherapeutic interventions on the basis of developmental psychopathology research taking into account knowledge of mediators and moderators. Developmental neuroscience research might be instrumental to uncover associated aberrant brain processes in children and adolescents with mental health problems and to better examine mechanisms of their correction by means of psychotherapy and psychological interventions. (4) Psychotherapy research needs to broaden in terms of adoption of large-scale public health strategies and treatments that can be applied to more patients in a simpler and cost-effective way. Increased research on efficacy and moderators of Internet-based treatments and e-mental health tools (e.g. to support real

Published
2014
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3. The need for a behavioural science focus in research on mental health and mental disorders

Author

Wittchen, Hans-Ulrich, Knappe, Susanne, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Bech, Per, Beckers, Tom, Berger, Thomas, Berking, Matthias, Berrocal, Carmen, Botella, Christina, Carlbring, Per, Chouinard, Guy, Colom, Francesc, Csillag, Claudio, Cujipers, Pim, David, Daniel, Emmelkamp, Paul M. G., A. Essau, Cecilia, Fava, Giovanni A., Goschke, Thomas, Hermans, Dirk, Hofmann, Stefan G., Lutz, Wolfgang, Muris, Peter, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Tossani, Eliana, van der Oord, Saskia, Vervliet, Bram, Haro, Josep M., Schumann, Gunter, Wittchen, Hans-Ulrich, Knappe, Susanne, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Bech, Per, Beckers, Tom, Berger, Thomas, Berking, Matthias, Berrocal, Carmen, Botella, Christina, Carlbring, Per, Chouinard, Guy, Colom, Francesc, Csillag, Claudio, Cujipers, Pim, David, Daniel, Emmelkamp, Paul M. G., A. Essau, Cecilia, Fava, Giovanni A., Goschke, Thomas, Hermans, Dirk, Hofmann, Stefan G., Lutz, Wolfgang, Muris, Peter, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Tossani, Eliana, van der Oord, Saskia, Vervliet, Bram, Haro, Josep M., and Schumann, Gunter

Abstract

Psychology as a science offers an enormous diversity of theories, principles, and methodological approaches to understand mental health, abnormal functions and behaviours and mental disorders. A selected overview of the scope, current topics as well as strength and gaps in Psychological Science may help to depict the advances needed to inform future research agendas specifically on mental health and mental disorders. From an integrative psychological perspective, most maladaptive health behaviours and mental disorders can be conceptualized as the result of developmental dysfunctions of psychological functions and processes as well as neurobiological and genetic processes that interact with the environment. The paper presents and discusses an integrative translational model, linking basic and experimental research with clinical research as well as population-based prospective-longitudinal studies. This model provides a conceptual framework to identify how individual vulnerabilities interact with environment over time, and promote critical behaviours that might act as proximal risk factors for ill-health and mental disorders. Within the models framework, such improved knowledge is also expected to better delineate targeted preventive and therapeutic interventions that prevent further escalation in early stages before the full disorder and further complications thereof develop. In contrast to conventional personalized medicine that typically targets individual (genetic) variation of patients who already have developed a disease to improve medical treatment, the proposed framework model, linked to a concerted funding programme of the Science of Behaviour Change, carries the promise of improved diagnosis, treatment and prevention of health-risk behaviour constellations as well as mental disorders.

Published
2014
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4. The need for a behavioural science focus in research on mental health and mental disorders

Author

Wittchen, Hans-Ulrich, Knappe, Susanne, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Bech, Per, Beckers, Tom, Berger, Thomas, Berking, Matthias, Berrocal, Carmen, Botella, Christina, Carlbring, Per, Chouinard, Guy, Colom, Francesc, Csillag, Claudio, Cujipers, Pim, David, Daniel, Emmelkamp, Paul M. G., A. Essau, Cecilia, Fava, Giovanni A., Goschke, Thomas, Hermans, Dirk, Hofmann, Stefan G., Lutz, Wolfgang, Muris, Peter, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Tossani, Eliana, van der Oord, Saskia, Vervliet, Bram, Haro, Josep M., Schumann, Gunter, Wittchen, Hans-Ulrich, Knappe, Susanne, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Bech, Per, Beckers, Tom, Berger, Thomas, Berking, Matthias, Berrocal, Carmen, Botella, Christina, Carlbring, Per, Chouinard, Guy, Colom, Francesc, Csillag, Claudio, Cujipers, Pim, David, Daniel, Emmelkamp, Paul M. G., A. Essau, Cecilia, Fava, Giovanni A., Goschke, Thomas, Hermans, Dirk, Hofmann, Stefan G., Lutz, Wolfgang, Muris, Peter, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Tossani, Eliana, van der Oord, Saskia, Vervliet, Bram, Haro, Josep M., and Schumann, Gunter

Abstract

Psychology as a science offers an enormous diversity of theories, principles, and methodological approaches to understand mental health, abnormal functions and behaviours and mental disorders. A selected overview of the scope, current topics as well as strength and gaps in Psychological Science may help to depict the advances needed to inform future research agendas specifically on mental health and mental disorders. From an integrative psychological perspective, most maladaptive health behaviours and mental disorders can be conceptualized as the result of developmental dysfunctions of psychological functions and processes as well as neurobiological and genetic processes that interact with the environment. The paper presents and discusses an integrative translational model, linking basic and experimental research with clinical research as well as population-based prospective-longitudinal studies. This model provides a conceptual framework to identify how individual vulnerabilities interact with environment over time, and promote critical behaviours that might act as proximal risk factors for ill-health and mental disorders. Within the models framework, such improved knowledge is also expected to better delineate targeted preventive and therapeutic interventions that prevent further escalation in early stages before the full disorder and further complications thereof develop. In contrast to conventional personalized medicine that typically targets individual (genetic) variation of patients who already have developed a disease to improve medical treatment, the proposed framework model, linked to a concerted funding programme of the Science of Behaviour Change, carries the promise of improved diagnosis, treatment and prevention of health-risk behaviour constellations as well as mental disorders.

Published
2014
Full Text
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5. Advancing psychotherapy and evidence-based psychological interventions

Author

Emmelkamp, Paul M. G., David, Daniel, Beckers, Tom, Muris, Peter, Cuijpers, Pim, Lutz, Wolfgang, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Berking, Matthias, Berger, Thomas, Botella, Christina, Carlbring, Per, Colom, Francesc, Essau, Cecilia, Hermans, Dirk, Hofmann, Stefan G., Knappe, Susanne, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Van der Oord, Saskia, Vervliet, Bram, Emmelkamp, Paul M. G., David, Daniel, Beckers, Tom, Muris, Peter, Cuijpers, Pim, Lutz, Wolfgang, Andersson, Gerhard, Araya, Ricardo, Banos Rivera, Rosa M., Barkham, Michael, Berking, Matthias, Berger, Thomas, Botella, Christina, Carlbring, Per, Colom, Francesc, Essau, Cecilia, Hermans, Dirk, Hofmann, Stefan G., Knappe, Susanne, Ollendick, Thomas H., Raes, Filip, Rief, Winfried, Riper, Heleen, Van der Oord, Saskia, and Vervliet, Bram

Abstract

Psychological models of mental disorders guide research into psychological and environmental factors that elicit and maintain mental disorders as well as interventions to reduce them. This paper addresses four areas. (1) Psychological models of mental disorders have become increasingly transdiagnostic, focusing on core cognitive endophenotypes of psychopathology from an integrative cognitive psychology perspective rather than offering explanations for unitary mental disorders. It is argued that psychological interventions for mental disorders will increasingly target specific cognitive dysfunctions rather than symptom-based mental disorders as a result. (2) Psychotherapy research still lacks a comprehensive conceptual framework that brings together the wide variety of findings, models and perspectives. Analysing the state-of-the-art in psychotherapy treatment research, component analyses aiming at an optimal identification of core ingredients and the mechanisms of change is highlighted as the core need towards improved efficacy and effectiveness of psychotherapy, and improved translation to routine care. (3) In order to provide more effective psychological interventions to children and adolescents, there is a need to develop new and/or improved psychotherapeutic interventions on the basis of developmental psychopathology research taking into account knowledge of mediators and moderators. Developmental neuroscience research might be instrumental to uncover associated aberrant brain processes in children and adolescents with mental health problems and to better examine mechanisms of their correction by means of psychotherapy and psychological interventions. (4) Psychotherapy research needs to broaden in terms of adoption of large-scale public health strategies and treatments that can be applied to more patients in a simpler and cost-effective way. Increased research on efficacy and moderators of Internet-based treatments and e-mental health tools (e.g. to support real

Published
2014
Full Text
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6. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

Author

Klionsky, Daniel J., Abeliovich, Hagai, Agostinis, Patrizia, Agrawal, Devendra K., Aliev, Giumrakch, S. Askew, David, Baba, Misuzu, H. Baehrecke, Eric, A. Bahr, Ben, Ballabio, Andrea, A. Bamber, Bruce, C. Bassham, Diane, Bergamini, Ettore, Bi, Xiaoning, Biard-Piechaczyk, Martine, S. Blum, Janice, E. Breclesen, Dale, L. Brodsky, Jeffrey, H. Brumell, John, Brunk, Ulf T., Bursch, Wilfried, Camougrand, Nadine, Cebollero, Eduardo, Cecconi, Francesco, Chen, Yingyu, Chin, Lih-Shen, Choi, Augustine, T. Chu, Charleen, Chung, Jongkyeong, G. H. Clarke, Peter, S. B. Clark, Robert, G. Clarke, Steven, Clave, Corinne, L. Cleveland, John, Codogno, Patrice, I. Colombo, Maria, Coto-Montes, Ana, M. Cregg, James, Maria Cuervo, Ana, Debnath, Jayanta, Demarchi, Francesca, B. Dennis, Patrick, A. Dennis, Phillip, Deretic, Vojo, J. Devenish, Rodney, Di Sano, Federica, Fred Dice, J., DiFiglia, Marian, Dinesh-Kumar, Savithramma, W. Distelhorst, Clark, Djavaheri-Mergny, Mojgan, C. Dorsey, Frank, Droege, Wulf, Dron, Michel, A. Jr. Dunn, William, Duszenko, Michael, Tony Eissa, N., Elazar, Zvulun, Esclatine, Audrey, Eskelinen, Eeva-Liisa, Fesues, Laszlo, D. Finley, Kim, M. Fuentes, Jose, Fueyo, Juan, Fujisaki, Kozo, Galliot, Brigitte, Gao, Fen-Biao, A. Gewirtz, David, B. Gibson, Spencer, Gohla, Antje, L. Goldberg, Alfred, Gonzalez, Ramon, Gonzalez-Estevez, Cristina, Gorski, Sharon, A. Gottlieb, Roberta, Haussinger, Dieter, He, You-Wen, Heidenreich, Kim, A. Hill, Joseph, Hoyer-Hansen, Maria, Hu, Xun, Huang, Wei-Pang, Iwasaki, Akiko, Jaattela, Marja, T. Jackson, William, Jiang, Xuejun, Jin, Shengkan, Johansen, Terje, U. Jung, Jae, Kadowaki, Motoni, Kang, Chanhee, Kelekar, Ameeta, H. Kessel, David, A. K. W. Kiel, Jan, Pyo Kim, Hong, Kimchi, Adi, J. Kinsella, Timothy, Kiselyov, Kirill, Kitamoto, Katsuhiko, Knecht, Erwin, Komatsu, Masaaki, Kominami, Eiki, Kondo, Seiji, L. Kovacs, Attila, Kroemer, Guido, Kuan, Chia-Yi, Kumar, Rakesh, Kundu, Mondira, Landry, Jacques, Laporte, Marianne, Le, Weidong, Lei, Huan-Yao, J. Lenardo, Michael, Levine, Beth, Lieberman, Andrew, Lim, Kah-Leong, Lin, Fu-Cheng, Liou, Willisa, F. Liu, Leroy, Lopez-Berestein, Gabriel, Lopez-Otin, Carlos, Lu, Bo, F. Macleod, Kay, Malorni, Walter, Martinet, Wim, Matsuoka, Ken, Mautner, Josef, J. Meijer, Alfred, Melendez, Alicia, Michels, Paul, Miotto, Giovanni, P. Mistiaen, Wilhelm, Mizushima, Noboru, Mograbi, Baharia, Monastyrska, Iryna, N. Moore, Michael, I. Moreira, Paula, Moriyasu, Yuji, Motyl, Tomasz, Muenz, Christian, O. Murphy, Leon, I. Naqvi, Naweed, Neufeld, Thomas, Nishino, Ichizo, A. Nixon, Ralph, Noda, Takeshi, Nuernberg, Bernd, Ogawa, Michinaga, L. Oleinick, Nancy, J. Olsen, Laura, Ozpolat, Bulent, Paglin, Shoshana, E. Palmer, Glen, Papassideri, Issidora, Parkes, Miles, H. Perlmutter, David, Perry, George, Piacentini, Mauro, Pinkas-Kramarski, Ronit, Prescott, Mark, Proikas-Cezanne, Tassula, Raben, Nina, Rami, Abdelhaq, Reggiori, Fulvio, Rohrer, Baerbel, C. Rubinsztein, David, M. Ryan, Kevin, Sadoshima, Junichi, Sakagami, Hiroshi, Sakai, Yasuyoshi, Sandri, Marco, Sasakawa, Chihiro, Sass, Miklos, Schneider, Claudio, O. Seglen, Per, Seleverstov, Oleksandr, Settleman, Jeffre, J. Shacka, John, M. Shapiro, Irving, Sibirny, Andrei, C. M. Silva-Zacarin, Elaine, Simon, Hans-Uwe, Simone, Cristiano, Simonsen, Anne, A. Smith, Mark, Spanel-Borowski, Katharina, Srinivas, Vickram, Steeves, Meredith, Stenmark, Harald, E. Stromhaug, Per, S. Subauste, Carlos, Sugimoto, Seiichiro, Sulzer, David, Suzuki, Toshihiko, S. Swanson, Michele, Takeshita, Fumihiko, J. Talbot, Nicholas., Talloczy, Zsolt, Tanaka, Keiji, Tanaka, Kozo, Tanida, Isei, S. Taylor, Graham, Paul Taylor, J., Terman, Alexei, Tettamanti, Gianluca, B. Thompson, Craig, Thumm, Michael, M. Tolkovsky, Aviva, A. Tooze, Sharon, Truant, Ray, V. Tumanovska, Lesya, Uchiyama, Yasuo, Ueno, Takashi, L. Uzcategui, Nestor, van der Klei, Ida, C. Vaquero, Eva, Vellai, Tibor, W. Vogel, Michael, Wang, Hong-Gang, Webster, Paul, W. Wiley, John, Xi, Zhijun, Xiao, Gutian, Yahalom, Joachim, Yang, Jin-Ming, Yap, George, Yin, Xiao-Min, Yoshimori, Tamotsu, Yu, Li, Yue, Zhenyu, Yuzaki, Michisuke, Zabirnyk, Olga, Zheng, Xiaoxiang, Zhu, Xiongwei, L. Deter, Russell, Tabas, Ira, Klionsky, Daniel J., Abeliovich, Hagai, Agostinis, Patrizia, Agrawal, Devendra K., Aliev, Giumrakch, S. Askew, David, Baba, Misuzu, H. Baehrecke, Eric, A. Bahr, Ben, Ballabio, Andrea, A. Bamber, Bruce, C. Bassham, Diane, Bergamini, Ettore, Bi, Xiaoning, Biard-Piechaczyk, Martine, S. Blum, Janice, E. Breclesen, Dale, L. Brodsky, Jeffrey, H. Brumell, John, Brunk, Ulf T., Bursch, Wilfried, Camougrand, Nadine, Cebollero, Eduardo, Cecconi, Francesco, Chen, Yingyu, Chin, Lih-Shen, Choi, Augustine, T. Chu, Charleen, Chung, Jongkyeong, G. H. Clarke, Peter, S. B. Clark, Robert, G. Clarke, Steven, Clave, Corinne, L. Cleveland, John, Codogno, Patrice, I. Colombo, Maria, Coto-Montes, Ana, M. Cregg, James, Maria Cuervo, Ana, Debnath, Jayanta, Demarchi, Francesca, B. Dennis, Patrick, A. Dennis, Phillip, Deretic, Vojo, J. Devenish, Rodney, Di Sano, Federica, Fred Dice, J., DiFiglia, Marian, Dinesh-Kumar, Savithramma, W. Distelhorst, Clark, Djavaheri-Mergny, Mojgan, C. Dorsey, Frank, Droege, Wulf, Dron, Michel, A. Jr. Dunn, William, Duszenko, Michael, Tony Eissa, N., Elazar, Zvulun, Esclatine, Audrey, Eskelinen, Eeva-Liisa, Fesues, Laszlo, D. Finley, Kim, M. Fuentes, Jose, Fueyo, Juan, Fujisaki, Kozo, Galliot, Brigitte, Gao, Fen-Biao, A. Gewirtz, David, B. Gibson, Spencer, Gohla, Antje, L. Goldberg, Alfred, Gonzalez, Ramon, Gonzalez-Estevez, Cristina, Gorski, Sharon, A. Gottlieb, Roberta, Haussinger, Dieter, He, You-Wen, Heidenreich, Kim, A. Hill, Joseph, Hoyer-Hansen, Maria, Hu, Xun, Huang, Wei-Pang, Iwasaki, Akiko, Jaattela, Marja, T. Jackson, William, Jiang, Xuejun, Jin, Shengkan, Johansen, Terje, U. Jung, Jae, Kadowaki, Motoni, Kang, Chanhee, Kelekar, Ameeta, H. Kessel, David, A. K. W. Kiel, Jan, Pyo Kim, Hong, Kimchi, Adi, J. Kinsella, Timothy, Kiselyov, Kirill, Kitamoto, Katsuhiko, Knecht, Erwin, Komatsu, Masaaki, Kominami, Eiki, Kondo, Seiji, L. Kovacs, Attila, Kroemer, Guido, Kuan, Chia-Yi, Kumar, Rakesh, Kundu, Mondira, Landry, Jacques, Laporte, Marianne, Le, Weidong, Lei, Huan-Yao, J. Lenardo, Michael, Levine, Beth, Lieberman, Andrew, Lim, Kah-Leong, Lin, Fu-Cheng, Liou, Willisa, F. Liu, Leroy, Lopez-Berestein, Gabriel, Lopez-Otin, Carlos, Lu, Bo, F. Macleod, Kay, Malorni, Walter, Martinet, Wim, Matsuoka, Ken, Mautner, Josef, J. Meijer, Alfred, Melendez, Alicia, Michels, Paul, Miotto, Giovanni, P. Mistiaen, Wilhelm, Mizushima, Noboru, Mograbi, Baharia, Monastyrska, Iryna, N. Moore, Michael, I. Moreira, Paula, Moriyasu, Yuji, Motyl, Tomasz, Muenz, Christian, O. Murphy, Leon, I. Naqvi, Naweed, Neufeld, Thomas, Nishino, Ichizo, A. Nixon, Ralph, Noda, Takeshi, Nuernberg, Bernd, Ogawa, Michinaga, L. Oleinick, Nancy, J. Olsen, Laura, Ozpolat, Bulent, Paglin, Shoshana, E. Palmer, Glen, Papassideri, Issidora, Parkes, Miles, H. Perlmutter, David, Perry, George, Piacentini, Mauro, Pinkas-Kramarski, Ronit, Prescott, Mark, Proikas-Cezanne, Tassula, Raben, Nina, Rami, Abdelhaq, Reggiori, Fulvio, Rohrer, Baerbel, C. Rubinsztein, David, M. Ryan, Kevin, Sadoshima, Junichi, Sakagami, Hiroshi, Sakai, Yasuyoshi, Sandri, Marco, Sasakawa, Chihiro, Sass, Miklos, Schneider, Claudio, O. Seglen, Per, Seleverstov, Oleksandr, Settleman, Jeffre, J. Shacka, John, M. Shapiro, Irving, Sibirny, Andrei, C. M. Silva-Zacarin, Elaine, Simon, Hans-Uwe, Simone, Cristiano, Simonsen, Anne, A. Smith, Mark, Spanel-Borowski, Katharina, Srinivas, Vickram, Steeves, Meredith, Stenmark, Harald, E. Stromhaug, Per, S. Subauste, Carlos, Sugimoto, Seiichiro, Sulzer, David, Suzuki, Toshihiko, S. Swanson, Michele, Takeshita, Fumihiko, J. Talbot, Nicholas., Talloczy, Zsolt, Tanaka, Keiji, Tanaka, Kozo, Tanida, Isei, S. Taylor, Graham, Paul Taylor, J., Terman, Alexei, Tettamanti, Gianluca, B. Thompson, Craig, Thumm, Michael, M. Tolkovsky, Aviva, A. Tooze, Sharon, Truant, Ray, V. Tumanovska, Lesya, Uchiyama, Yasuo, Ueno, Takashi, L. Uzcategui, Nestor, van der Klei, Ida, C. Vaquero, Eva, Vellai, Tibor, W. Vogel, Michael, Wang, Hong-Gang, Webster, Paul, W. Wiley, John, Xi, Zhijun, Xiao, Gutian, Yahalom, Joachim, Yang, Jin-Ming, Yap, George, Yin, Xiao-Min, Yoshimori, Tamotsu, Yu, Li, Yue, Zhenyu, Yuzaki, Michisuke, Zabirnyk, Olga, Zheng, Xiaoxiang, Zhu, Xiongwei, L. Deter, Russell, and Tabas, Ira

Abstract

Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.

Published
2008

7. Guidelines for the use and interpretation of assays for monitoring autophagy in higher eukaryotes

Author

Klionsky, Daniel J., Abeliovich, Hagai, Agostinis, Patrizia, Agrawal, Devendra K., Aliev, Giumrakch, S. Askew, David, Baba, Misuzu, H. Baehrecke, Eric, A. Bahr, Ben, Ballabio, Andrea, A. Bamber, Bruce, C. Bassham, Diane, Bergamini, Ettore, Bi, Xiaoning, Biard-Piechaczyk, Martine, S. Blum, Janice, E. Breclesen, Dale, L. Brodsky, Jeffrey, H. Brumell, John, Brunk, Ulf T., Bursch, Wilfried, Camougrand, Nadine, Cebollero, Eduardo, Cecconi, Francesco, Chen, Yingyu, Chin, Lih-Shen, Choi, Augustine, T. Chu, Charleen, Chung, Jongkyeong, G. H. Clarke, Peter, S. B. Clark, Robert, G. Clarke, Steven, Clave, Corinne, L. Cleveland, John, Codogno, Patrice, I. Colombo, Maria, Coto-Montes, Ana, M. Cregg, James, Maria Cuervo, Ana, Debnath, Jayanta, Demarchi, Francesca, B. Dennis, Patrick, A. Dennis, Phillip, Deretic, Vojo, J. Devenish, Rodney, Di Sano, Federica, Fred Dice, J., DiFiglia, Marian, Dinesh-Kumar, Savithramma, W. Distelhorst, Clark, Djavaheri-Mergny, Mojgan, C. Dorsey, Frank, Droege, Wulf, Dron, Michel, A. Jr. Dunn, William, Duszenko, Michael, Tony Eissa, N., Elazar, Zvulun, Esclatine, Audrey, Eskelinen, Eeva-Liisa, Fesues, Laszlo, D. Finley, Kim, M. Fuentes, Jose, Fueyo, Juan, Fujisaki, Kozo, Galliot, Brigitte, Gao, Fen-Biao, A. Gewirtz, David, B. Gibson, Spencer, Gohla, Antje, L. Goldberg, Alfred, Gonzalez, Ramon, Gonzalez-Estevez, Cristina, Gorski, Sharon, A. Gottlieb, Roberta, Haussinger, Dieter, He, You-Wen, Heidenreich, Kim, A. Hill, Joseph, Hoyer-Hansen, Maria, Hu, Xun, Huang, Wei-Pang, Iwasaki, Akiko, Jaattela, Marja, T. Jackson, William, Jiang, Xuejun, Jin, Shengkan, Johansen, Terje, U. Jung, Jae, Kadowaki, Motoni, Kang, Chanhee, Kelekar, Ameeta, H. Kessel, David, A. K. W. Kiel, Jan, Pyo Kim, Hong, Kimchi, Adi, J. Kinsella, Timothy, Kiselyov, Kirill, Kitamoto, Katsuhiko, Knecht, Erwin, Komatsu, Masaaki, Kominami, Eiki, Kondo, Seiji, L. Kovacs, Attila, Kroemer, Guido, Kuan, Chia-Yi, Kumar, Rakesh, Kundu, Mondira, Landry, Jacques, Laporte, Marianne, Le, Weidong, Lei, Huan-Yao, J. Lenardo, Michael, Levine, Beth, Lieberman, Andrew, Lim, Kah-Leong, Lin, Fu-Cheng, Liou, Willisa, F. Liu, Leroy, Lopez-Berestein, Gabriel, Lopez-Otin, Carlos, Lu, Bo, F. Macleod, Kay, Malorni, Walter, Martinet, Wim, Matsuoka, Ken, Mautner, Josef, J. Meijer, Alfred, Melendez, Alicia, Michels, Paul, Miotto, Giovanni, P. Mistiaen, Wilhelm, Mizushima, Noboru, Mograbi, Baharia, Monastyrska, Iryna, N. Moore, Michael, I. Moreira, Paula, Moriyasu, Yuji, Motyl, Tomasz, Muenz, Christian, O. Murphy, Leon, I. Naqvi, Naweed, Neufeld, Thomas, Nishino, Ichizo, A. Nixon, Ralph, Noda, Takeshi, Nuernberg, Bernd, Ogawa, Michinaga, L. Oleinick, Nancy, J. Olsen, Laura, Ozpolat, Bulent, Paglin, Shoshana, E. Palmer, Glen, Papassideri, Issidora, Parkes, Miles, H. Perlmutter, David, Perry, George, Piacentini, Mauro, Pinkas-Kramarski, Ronit, Prescott, Mark, Proikas-Cezanne, Tassula, Raben, Nina, Rami, Abdelhaq, Reggiori, Fulvio, Rohrer, Baerbel, C. Rubinsztein, David, M. Ryan, Kevin, Sadoshima, Junichi, Sakagami, Hiroshi, Sakai, Yasuyoshi, Sandri, Marco, Sasakawa, Chihiro, Sass, Miklos, Schneider, Claudio, O. Seglen, Per, Seleverstov, Oleksandr, Settleman, Jeffre, J. Shacka, John, M. Shapiro, Irving, Sibirny, Andrei, C. M. Silva-Zacarin, Elaine, Simon, Hans-Uwe, Simone, Cristiano, Simonsen, Anne, A. Smith, Mark, Spanel-Borowski, Katharina, Srinivas, Vickram, Steeves, Meredith, Stenmark, Harald, E. Stromhaug, Per, S. Subauste, Carlos, Sugimoto, Seiichiro, Sulzer, David, Suzuki, Toshihiko, S. Swanson, Michele, Takeshita, Fumihiko, J. Talbot, Nicholas., Talloczy, Zsolt, Tanaka, Keiji, Tanaka, Kozo, Tanida, Isei, S. Taylor, Graham, Paul Taylor, J., Terman, Alexei, Tettamanti, Gianluca, B. Thompson, Craig, Thumm, Michael, M. Tolkovsky, Aviva, A. Tooze, Sharon, Truant, Ray, V. Tumanovska, Lesya, Uchiyama, Yasuo, Ueno, Takashi, L. Uzcategui, Nestor, van der Klei, Ida, C. Vaquero, Eva, Vellai, Tibor, W. Vogel, Michael, Wang, Hong-Gang, Webster, Paul, W. Wiley, John, Xi, Zhijun, Xiao, Gutian, Yahalom, Joachim, Yang, Jin-Ming, Yap, George, Yin, Xiao-Min, Yoshimori, Tamotsu, Yu, Li, Yue, Zhenyu, Yuzaki, Michisuke, Zabirnyk, Olga, Zheng, Xiaoxiang, Zhu, Xiongwei, L. Deter, Russell, Tabas, Ira, Klionsky, Daniel J., Abeliovich, Hagai, Agostinis, Patrizia, Agrawal, Devendra K., Aliev, Giumrakch, S. Askew, David, Baba, Misuzu, H. Baehrecke, Eric, A. Bahr, Ben, Ballabio, Andrea, A. Bamber, Bruce, C. Bassham, Diane, Bergamini, Ettore, Bi, Xiaoning, Biard-Piechaczyk, Martine, S. Blum, Janice, E. Breclesen, Dale, L. Brodsky, Jeffrey, H. Brumell, John, Brunk, Ulf T., Bursch, Wilfried, Camougrand, Nadine, Cebollero, Eduardo, Cecconi, Francesco, Chen, Yingyu, Chin, Lih-Shen, Choi, Augustine, T. Chu, Charleen, Chung, Jongkyeong, G. H. Clarke, Peter, S. B. Clark, Robert, G. Clarke, Steven, Clave, Corinne, L. Cleveland, John, Codogno, Patrice, I. Colombo, Maria, Coto-Montes, Ana, M. Cregg, James, Maria Cuervo, Ana, Debnath, Jayanta, Demarchi, Francesca, B. Dennis, Patrick, A. Dennis, Phillip, Deretic, Vojo, J. Devenish, Rodney, Di Sano, Federica, Fred Dice, J., DiFiglia, Marian, Dinesh-Kumar, Savithramma, W. Distelhorst, Clark, Djavaheri-Mergny, Mojgan, C. Dorsey, Frank, Droege, Wulf, Dron, Michel, A. Jr. Dunn, William, Duszenko, Michael, Tony Eissa, N., Elazar, Zvulun, Esclatine, Audrey, Eskelinen, Eeva-Liisa, Fesues, Laszlo, D. Finley, Kim, M. Fuentes, Jose, Fueyo, Juan, Fujisaki, Kozo, Galliot, Brigitte, Gao, Fen-Biao, A. Gewirtz, David, B. Gibson, Spencer, Gohla, Antje, L. Goldberg, Alfred, Gonzalez, Ramon, Gonzalez-Estevez, Cristina, Gorski, Sharon, A. Gottlieb, Roberta, Haussinger, Dieter, He, You-Wen, Heidenreich, Kim, A. Hill, Joseph, Hoyer-Hansen, Maria, Hu, Xun, Huang, Wei-Pang, Iwasaki, Akiko, Jaattela, Marja, T. Jackson, William, Jiang, Xuejun, Jin, Shengkan, Johansen, Terje, U. Jung, Jae, Kadowaki, Motoni, Kang, Chanhee, Kelekar, Ameeta, H. Kessel, David, A. K. W. Kiel, Jan, Pyo Kim, Hong, Kimchi, Adi, J. Kinsella, Timothy, Kiselyov, Kirill, Kitamoto, Katsuhiko, Knecht, Erwin, Komatsu, Masaaki, Kominami, Eiki, Kondo, Seiji, L. Kovacs, Attila, Kroemer, Guido, Kuan, Chia-Yi, Kumar, Rakesh, Kundu, Mondira, Landry, Jacques, Laporte, Marianne, Le, Weidong, Lei, Huan-Yao, J. Lenardo, Michael, Levine, Beth, Lieberman, Andrew, Lim, Kah-Leong, Lin, Fu-Cheng, Liou, Willisa, F. Liu, Leroy, Lopez-Berestein, Gabriel, Lopez-Otin, Carlos, Lu, Bo, F. Macleod, Kay, Malorni, Walter, Martinet, Wim, Matsuoka, Ken, Mautner, Josef, J. Meijer, Alfred, Melendez, Alicia, Michels, Paul, Miotto, Giovanni, P. Mistiaen, Wilhelm, Mizushima, Noboru, Mograbi, Baharia, Monastyrska, Iryna, N. Moore, Michael, I. Moreira, Paula, Moriyasu, Yuji, Motyl, Tomasz, Muenz, Christian, O. Murphy, Leon, I. Naqvi, Naweed, Neufeld, Thomas, Nishino, Ichizo, A. Nixon, Ralph, Noda, Takeshi, Nuernberg, Bernd, Ogawa, Michinaga, L. Oleinick, Nancy, J. Olsen, Laura, Ozpolat, Bulent, Paglin, Shoshana, E. Palmer, Glen, Papassideri, Issidora, Parkes, Miles, H. Perlmutter, David, Perry, George, Piacentini, Mauro, Pinkas-Kramarski, Ronit, Prescott, Mark, Proikas-Cezanne, Tassula, Raben, Nina, Rami, Abdelhaq, Reggiori, Fulvio, Rohrer, Baerbel, C. Rubinsztein, David, M. Ryan, Kevin, Sadoshima, Junichi, Sakagami, Hiroshi, Sakai, Yasuyoshi, Sandri, Marco, Sasakawa, Chihiro, Sass, Miklos, Schneider, Claudio, O. Seglen, Per, Seleverstov, Oleksandr, Settleman, Jeffre, J. Shacka, John, M. Shapiro, Irving, Sibirny, Andrei, C. M. Silva-Zacarin, Elaine, Simon, Hans-Uwe, Simone, Cristiano, Simonsen, Anne, A. Smith, Mark, Spanel-Borowski, Katharina, Srinivas, Vickram, Steeves, Meredith, Stenmark, Harald, E. Stromhaug, Per, S. Subauste, Carlos, Sugimoto, Seiichiro, Sulzer, David, Suzuki, Toshihiko, S. Swanson, Michele, Takeshita, Fumihiko, J. Talbot, Nicholas., Talloczy, Zsolt, Tanaka, Keiji, Tanaka, Kozo, Tanida, Isei, S. Taylor, Graham, Paul Taylor, J., Terman, Alexei, Tettamanti, Gianluca, B. Thompson, Craig, Thumm, Michael, M. Tolkovsky, Aviva, A. Tooze, Sharon, Truant, Ray, V. Tumanovska, Lesya, Uchiyama, Yasuo, Ueno, Takashi, L. Uzcategui, Nestor, van der Klei, Ida, C. Vaquero, Eva, Vellai, Tibor, W. Vogel, Michael, Wang, Hong-Gang, Webster, Paul, W. Wiley, John, Xi, Zhijun, Xiao, Gutian, Yahalom, Joachim, Yang, Jin-Ming, Yap, George, Yin, Xiao-Min, Yoshimori, Tamotsu, Yu, Li, Yue, Zhenyu, Yuzaki, Michisuke, Zabirnyk, Olga, Zheng, Xiaoxiang, Zhu, Xiongwei, L. Deter, Russell, and Tabas, Ira

Abstract

Research in autophagy continues to accelerate,1 and as a result many new scientists are entering the field. Accordingly, it is important to establish a standard set of criteria for monitoring macroautophagy in different organisms. Recent reviews have described the range of assays that have been used for this purpose.2,3 There are many useful and convenient methods that can be used to monitor macroautophagy in yeast, but relatively few in other model systems, and there is much confusion regarding acceptable methods to measure macroautophagy in higher eukaryotes. A key point that needs to be emphasized is that there is a difference between measurements that monitor the numbers of autophagosomes versus those that measure flux through the autophagy pathway; thus, a block in macroautophagy that results in autophagosome accumulation needs to be differentiated from fully functional autophagy that includes delivery to, and degradation within, lysosomes (in most higher eukaryotes) or the vacuole (in plants and fungi). Here, we present a set of guidelines for the selection and interpretation of the methods that can be used by investigators who are attempting to examine macroautophagy and related processes, as well as by reviewers who need to provide realistic and reasonable critiques of papers that investigate these processes. This set of guidelines is not meant to be a formulaic set of rules, because the appropriate assays depend in part on the question being asked and the system being used. In addition, we emphasize that no individual assay is guaranteed to be the most appropriate one in every situation, and we strongly recommend the use of multiple assays to verify an autophagic response.

Published
2008