1. Epitope-Based Peptide Vaccine against Glycoprotein G of Nipah Henipavirus Using Immunoinformatics Approaches.
- Author
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Mohammed AA, Shantier SW, Mustafa MI, Osman HK, Elmansi HE, Osman IA, Mohammed RA, Abdelrhman FA, Elnnewery ME, Yousif EM, Mustafa MM, Elfadol NM, Abdalla AI, Mahmoud E, Yagaub AA, Ahmed YA, and Hassan MA
- Subjects
- Antigens, Viral metabolism, Asia, Southeastern epidemiology, Computational Biology, Endemic Diseases, Epitope Mapping, Epitopes immunology, Epitopes metabolism, Glycoside Hydrolases metabolism, HLA Antigens metabolism, Henipavirus Infections epidemiology, Humans, Malaysia epidemiology, Molecular Docking Simulation, Protein Binding, Respiratory Tract Infections, Vaccination, Antigens, Viral genetics, Epitopes genetics, Glycoside Hydrolases genetics, Henipavirus Infections immunology, Nipah Virus physiology, Vaccines, Subunit immunology, Viral Vaccines immunology
- Abstract
Background: Nipah belongs to the genus Henipavirus and the Paramyxoviridae family . It is an endemic most commonly found at South Asia and has first emerged in Malaysia in 1998. Bats are found to be the main reservoir for this virus, causing disease in both humans and animals. The last outbreak has occurred in May 2018 in Kerala. It is characterized by high pathogenicity and fatality rates which varies from 40% to 70% depending on the severity of the disease and on the availability of adequate healthcare facilities. Currently, there are no antiviral drugs available for NiV disease and the treatment is just supportive. Clinical presentations for this virus range from asymptomatic infection to fatal encephalitis., Objective: This study is aimed at predicting an effective epitope-based vaccine against glycoprotein G of Nipah henipavirus, using immunoinformatics approaches., Methods and Materials: Glycoprotein G of the Nipah virus sequence was retrieved from NCBI. Different prediction tools were used to analyze the epitopes, namely, BepiPred-2.0: Sequential B Cell Epitope Predictor for B cell and T cell MHC classes II and I. Then, the proposed peptides were docked using Autodock 4.0 software program. Results and Conclusions . The two peptides TVYHCSAVY and FLIDRINWI have showed a very strong binding affinity to MHC class I and MHC class II alleles. Furthermore, considering the conservancy, the affinity, and the population coverage, the peptide FLIDRINWIT is highly suitable to be utilized to formulate a new vaccine against glycoprotein G of Nipah henipavirus. An in vivo study for the proposed peptides is also highly recommended., Competing Interests: The authors declare that they have no competing interests., (Copyright © 2020 Arwa A. Mohammed et al.)
- Published
- 2020
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