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The present study assesses the efficacy of SVANOVIRTheileria annulata-Ab, the first commercial ELISA kit for the diagnosis ofTheileria annulatainfection in cattle based on a recombinant protein known asT. annulatasurface protein (TaSp). As a reference test, a polymerase chain reaction (PCR) assay depending onT. annulatamerozoite surface antigen (Tams-1) was applied. A total of 468 blood samples as well as serum samples were randomly collected from cattle and tested in the PCR as well as in the ELISA developed in this study. Moreover, all samples were also analyzed by conventional Giemsa-stained blood smear. The results of this study revealed a good correlation between the results obtained by PCR and the ELISA, whereas all PCR positive samples scored correctly positive in the ELISA and 73 of the 125 PCR negative samples scored correctly negative. Taken together, a sensitivity of 91.25% and a specificity of 78.4% were recorded, when compared to the PCR data. In conclusion, the SVANOVIRTheileria annulata-Ab is a suitable diagnostic assay for use in the diagnosis and epidemiological surveys ofTheileria annulatainfection in chronic and carrier animals.