1. Ginseng-Sanqi-Chuanxiong (GSC) Extracts Ameliorate Diabetes-Induced Endothelial Cell Senescence through Regulating Mitophagy via the AMPK Pathway.
- Author
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Wang X, Zhang JQ, Xiu CK, Yang J, Fang JY, and Lei Y
- Subjects
- AMP-Activated Protein Kinases antagonists & inhibitors, Autophagy drug effects, Cell Survival drug effects, Drugs, Chinese Herbal chemistry, Endothelial Cells drug effects, Endothelial Cells metabolism, Glucose toxicity, Humans, Mitochondria drug effects, Mitochondria metabolism, Models, Biological, Palmitic Acid toxicity, Plant Extracts chemistry, Quinazolinones pharmacology, RNA, Small Interfering metabolism, AMP-Activated Protein Kinases metabolism, Cellular Senescence drug effects, Diabetes Mellitus pathology, Drugs, Chinese Herbal pharmacology, Endothelial Cells enzymology, Endothelial Cells pathology, Mitophagy drug effects, Plant Extracts pharmacology, Signal Transduction drug effects
- Abstract
Vascular endothelial senescence induced by high glucose and palmitate (HG/PA) contributes to endothelial dysfunction, which leads to diabetic cardiovascular complications. Reduction of endothelial senescence may attenuate these pathogenic processes. This study is aimed at determining whether Ginseng-Sanqi-Chuanxiong (GSC) extracts, traditional Chinese medicine, can ameliorate human aortic endothelial cell (HAEC) senescence under HG/PA-stressed conditions and further explore the underlying mechanism. We found that GSC extracts significantly increased antisenescent activity by reducing the HG/PA-induced mitochondrial ROS (mtROS) levels in senescent HAECs. GSC extracts also induced cellular mitophagy formation, which mediated the effect of GSC extracts on mtROS reduction. Apart from this, the data showed that GSC extracts stimulated mitophagy via the AMPK pathway, and upon inhibition of AMPK by pharmacological and genetic inhibitors, GSC extract-mediated mitophagy was abolished which further led to reverse the antisenescence effect. Taken together, these data suggest that GSC extracts prevent HG/PA-induced endothelial senescence and mtROS production by mitophagy regulation via the AMPK pathway. Thus, the induction of mitophagy by GSC extracts may provide a novel therapeutic candidate for cardiovascular protection in metabolic syndrome., Competing Interests: All authors have declared no conflicts of interest., (Copyright © 2020 Xue Wang et al.)
- Published
- 2020
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