1. Suppressions of Migration and Invasion by Cantharidin in TSGH-8301 Human Bladder Carcinoma Cells through the Inhibitions of Matrix Metalloproteinase-2/-9 Signaling.
- Author
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Yi-Ping Huang, Chien-Hang Ni, Chi-Cheng Lu, Jo-Hua Chiang, Jai-Sing Yang, Yang-Ching Ko, Jing-Pin Lin, Jehn-Hwa Kuo, Shu-Jen Chang, and Jing-Gung Chung
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ENZYME metabolism , *ANALYSIS of variance , *CANTHARIDES , *CELLULAR signal transduction , *GENE expression , *METASTASIS , *POLYMERASE chain reaction , *RESEARCH funding , *STATISTICS , *WESTERN immunoblotting , *DATA analysis , *DESCRIPTIVE statistics , *IN vitro studies ,BLADDER tumors - Abstract
Cancer metastasis becomes an initial cause of cancer death in human population. In many cancers, it has been shown that the high levels of matrix metalloproteinase (MMP)-2 and/or MMP-9 are associated with the invasive phenotypes of cancer cells. In this study, we investigated the effects of cantharidin, a derivative of blister beetles which is one of the traditional Chinese medicines, on the adhesion, migration, and invasion of human bladder cancer TSGH-8301 cells. Cantharidin effectively suppressed TSGH-8301 cell adhesion, migration, and invasion in a concentration-dependent manner. Results from Western blotting, RT-PCR, and gelatin zymography assays indicated that cantharidin blocked the protein levels, gene expression (mRNA), and activities of MMP-2 and -9 in TSGH-8301 cells. Cantharidin also significantly suppressed the protein expressions of p-p38 and p-JNKl/2 in TSGH-8301 cells. Taken together, cantharidin was suggested to present antimetastatic potential via suppressing the levels of MMP-2 and MMP-9 expression that might be mediated by targeting the p38 and JNK1/2 MAPKs pathway in TSGH-8301 human bladder cancer cells. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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