1. Membrane stability and mitochondrial activity of human-ejaculated spermatozoa during in vitro experimental infection with Escherichia coli, Staphylococcus haemolyticus and Bacteroides ureolyticus
- Author
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Monika Fraczek, S. Lenart, Dariusz Gaczarzewicz, Anna Szumala-Kakol, Małgorzata Piasecka, Maria Laszczyńska, Maciej Kurpisz, and Anna Kazienko
- Subjects
Adult ,Male ,endocrine system ,Cell Survival ,Urology ,ved/biology.organism_classification_rank.species ,Pyrimidinones ,Mitochondrion ,Biology ,medicine.disease_cause ,Microbiology ,Andrology ,Endocrinology ,medicine ,Humans ,Escherichia coli ,Escherichia coli Infections ,Infertility, Male ,reproductive and urinary physiology ,Sperm plasma membrane ,Membrane potential ,urogenital system ,ved/biology ,Cell Membrane ,General Medicine ,Carbocyanines ,Staphylococcal Infections ,Bacteroides Infections ,biology.organism_classification ,Spermatozoa ,Staphylococcus haemolyticus ,Sperm ,In vitro ,Mitochondria ,Benzimidazoles ,Bacteroides ureolyticus ,Bacteria - Abstract
Summary The aim of the study was to examine an in vitro effect of the three bacterial strains (Escherichia coli, Staphylococcus haemolyticus and Bacteroides ureolyticus) on ejaculated spermatozoa with reference to sperm membrane integrity and mitochondrial activity. The study was carried out on swim-up-separated spermatozoa from 12 normozoospermic volunteers. Sperm plasma membrane stability was evaluated by the LIVE/DEAD Sperm Viability Kit and by the merocyanine 540 test. Mitochondrial activity was evaluated using the JC-1 test as well as the NADH-dependent NBT assay. The percentage of dead cells was significantly higher in spermatozoa treated with B. ureolyticus as compared to that of control spermatozoa (P
- Published
- 2012