1. Acanthopanax versus 3-Methyladenine Ameliorates Sodium Taurocholate-Induced Severe Acute Pancreatitis by Inhibiting the Autophagic Pathway in Rats
- Author
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Chun Liu, Ronglong Wei, Guoxiong Zhou, Qing Miao, Xiaohong Wang, Shunxing Zhu, Mengjie Wu, and Yuefen Xu
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Article Subject ,Immunology ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,Western blot ,Internal medicine ,medicine ,lcsh:Pathology ,Phosphatidylinositol ,Amylase ,medicine.diagnostic_test ,Cell Biology ,medicine.disease ,Taurocholic acid ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,biology.protein ,Pancreatitis ,Acute pancreatitis ,Signal transduction ,Pancreas ,lcsh:RB1-214 - Abstract
Objectives. To observe the therapeutic effects of Acanthopanax and 3-methyladenine against severe acute pancreatitis (SAP).Methods. Sodium taurocholate-induced SAP rats were equally randomized into a SAP group, an Acanthopanax group, and a 3-methyladenine group. Serum amylase levels were determined by ELISA; protein and mRNA expression levels of nucleus nuclear factor kappa B (NF-κB) p65, light chain 3II (LC3-II), and Beclin-1 and mRNA expression levels of Class III phosphatidylinositol 3-kinase (PI3K-III) in pancreas tissue were detected by Western blot and quantitative real-time PCR, respectively; mortality and pathological change of the pancreas were observed at 3, 12, and 24 h after operation.Results. There was no significant difference in mortality between SAP group and both treatment groups (P>0.05). Serum amylase levels, protein, and mRNA expression levels of nucleus NF-κB p65, LC3-II, and Beclin-1 protein, mRNA expression levels of PI3K-III, and pathological score of the pancreas in both treatment groups were significantly lower than those in SAP group at 12 and 24 h after operation (P<0.05or 0.01). The number of autophagosomes and autophagolysosomes of pancreatic acinar cells in both treatment groups was smaller than that in SAP group at 12 and 24 h.Conclusions. Acanthopanax and 3-methyladenine had similar therapeutic effects against SAP in rats. The mechanism may be through inhibiting abnormal autophagy activation of pancreatic acinar cells.
- Published
- 2016