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An active packaging film based on low-density polyethylene (LDPE)/ethylene-vinyl acetate (EVA) copolymer was prepared. Oregano essential oil microcapsules were prepared by spray drying and added as active antibacterial ingredients to the LDPE/ EVA film by melt blending and extrusion casting and enhanced the moisture permeability and gas permeability of the film. The active packaging film effectively regulates the respiration of strawberries by delaying the accumulation of carbon dioxide and the reduction of oxygen in the package. The firmness, total soluble solids, titratable acid, malondialdehyde, and hydrogen peroxide content of strawberries were tested. Low-field nuclear magnetic resonance (LF-NMR), T2 relaxation time method, and a visualization method (MRI) were used to track the water status and migration of strawberry in different active film packages, and the change in the moisture content also indicated that the active film can effectively reduce the content and speed of free water produced by respiratory metabolism. [ABSTRACT FROM AUTHOR]