1. Improved physiological properties of gravity-enforced reassembled rat and human pancreatic pseudo-islets
- Author
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Roger Lehmann, Richard A. Zuellig, Oliver Tschopp, Wolfgang Moritz, Philipp A. Gerber, Giatgen A. Spinas, and Giuseppe Cavallari
- Subjects
0301 basic medicine ,endocrine system ,medicine.medical_specialty ,endocrine system diseases ,medicine.medical_treatment ,Cell ,Biomedical Engineering ,Medicine (miscellaneous) ,Biology ,Biomaterials ,03 medical and health sciences ,Internal medicine ,Diabetes mellitus ,medicine ,geography ,Islet cell transplantation ,geography.geographical_feature_category ,Insulin ,Islet ,medicine.disease ,Cell aggregation ,Transplantation ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Cell culture - Abstract
Previously we demonstrated the superiority of small islets vs large islets in terms of function and survival after transplantation, and we generated reaggregated rat islets (pseudo-islets) of standardized small dimensions by the hanging-drop culture method (HDCM). The aim of this study was to generate human pseudo-islets by HDCM and to evaluate and compare the physiological properties of rat and human pseudo-islets. Isolated rat and human islets were dissociated into single cells and incubated for 6-14 days by HDCM. Newly formed pseudo-islets were analysed for dimensions, morphology, glucose-stimulated insulin secretion (GSIS) and total insulin content. The morphology of reaggregated human islets was similar to that of native islets, while rat pseudo-islets had a reduced content of α and δ cells. GSIS of small rat and human pseudo-islets (250 cells) was increased up to 4.0-fold (p
- Published
- 2014