5 results on '"Kazuto HOSHI"'
Search Results
2. Therapeutic effects of a recombinant human collagen peptide bioscaffold with human adipose-derived stem cells on impaired wound healing after radiotherapy
- Author
-
Kotaro Yoshimura, Kensuke Tashiro, Tsuyoshi Takato, Akira Kurisaki, Jingwei Feng, Koji Kanayama, Hitomi Takada, Rintaro Asahi, Ataru Sunaga, Kazuto Hoshi, Takanobu Mashiko, and Szu-Hsien Wu
- Subjects
Adult ,Male ,0301 basic medicine ,Biomedical Engineering ,Mice, Nude ,Medicine (miscellaneous) ,Regenerative medicine ,Biomaterials ,Cell therapy ,Extracellular matrix ,03 medical and health sciences ,chemistry.chemical_compound ,medicine ,Animals ,Humans ,Cells, Cultured ,Mice, Inbred BALB C ,Wound Healing ,Tissue Scaffolds ,biology ,Chemistry ,Stem Cells ,Transforming growth factor beta ,Recombinant Proteins ,Cell biology ,Vascular endothelial growth factor ,Disease Models, Animal ,030104 developmental biology ,Adipose Tissue ,Gene Expression Regulation ,biology.protein ,Female ,Hepatocyte growth factor ,Collagen ,Stem cell ,Peptides ,Wound healing ,Biomarkers ,medicine.drug - Abstract
Chronic changes following radiotherapy include alterations in tissue-resident stem cells and vasculatures, which can lead to impaired wound healing. In this study, novel recombinant human collagen peptide (rhCP) scaffolds were evaluated as a biomaterial carrier for cellular regenerative therapy. Human adipose-derived stem cells (hASCs) were successfully cultured on rhCP scaffolds. By hASC culture on rhCP, microarray assay indicated that expression of genes related to cell proliferation and extracellular matrix production was upregulated. Pathway analyses revealed that signaling pathways related to inflammatory suppression and cell growth promotion were activated as well as signaling pathways consistent with some growth factors including vascular endothelial growth factor, hepatocyte growth factor, and transforming growth factor beta, although gene expression of these growth factors was not upregulated. These findings suggest the rhCP scaffold showed similar biological actions to cytokines regulating cell growth and immunity. In subsequent impaired wound healing experiments using a locally irradiated (20 Gray) mouse, wound treatment with rhCP sponges combined with cultured hASCs and human umbilical vein endothelial cells accelerated wound closure compared with wounds treated with rhCP with hASCs alone, rhCP only, and control (dressing alone), with better healing observed according to this order. These results indicating the therapeutic value of rhCP scaffolds as a topical biomaterial dressing and a biocarrier of stem cells and vascular endothelial cells for regenerating therapies. The combination of rhCP and functional cells was suggested to be a potential tool for revitalizing stem cell-depleted conditions such as radiation tissue damage.
- Published
- 2018
3. A Case of Odontogenic Infection byStreptococcus constellatusLeading to Systemic Infection in a Cogan’s Syndrome Patient
- Author
-
Kazuto Hoshi, Takahiro Abe, Kazumi Ohkubo, Yoshiyuki Mori, Masanobu Abe, Tsuyoshi Takato, Yae Ohata, Hideto Saijo, and Ryoko Inaki
- Subjects
Odontogenic infection ,medicine.medical_specialty ,S syndrome ,biology ,business.industry ,RK1-715 ,Case Report ,medicine.disease ,Streptococcus constellatus ,biology.organism_classification ,Chronic periodontitis ,Mandibular first molar ,Dermatology ,Surgery ,Sepsis ,Furcation Involvement ,stomatognathic system ,Chronic osteomyelitis ,Dentistry ,medicine ,business ,General Dentistry - Abstract
Odontogenic infection in immunocompromised patients tends to extend systemically beyond the oral cavity. Our case report presents a patient with sepsis due to aStreptococcus constellatus(S. constellatus) odontogenic infection in a 64-year-old-immunocompromised woman with Cogan’s syndrome. She had been suffering from chronic mandibular osteomyelitis which was thought to have been caused by dental caries and/or chronic periodontitis with furcation involvement of the left mandibular first molar. We suspect that the acute symptoms of the chronic osteomyelitis due toS. constellatusled to the systemic infection. This infection could be accelerated by the use of a corticosteroid and an alendronate. This is the first report which represents the potential association between odontogenic infection and Cogan’s syndrome.
- Published
- 2014
4. Tissue responses against tissue-engineered cartilage consisting of chondrocytes encapsulated within non-absorbable hydrogel
- Author
-
Satoru Nagata, Yukiyo Asawa, Yuko Fujihara, Makoto Komura, Sanshiro Kanazawa, Kazuto Hoshi, Tsuyoshi Takato, and Tomoaki Sakamoto
- Subjects
Dense connective tissue ,Pathology ,medicine.medical_specialty ,biology ,Cartilage ,Biomedical Engineering ,Medicine (miscellaneous) ,Chondrogenesis ,Biomaterials ,Transplantation ,chemistry.chemical_compound ,medicine.anatomical_structure ,chemistry ,Immunology ,biology.protein ,medicine ,Cytotoxic T cell ,Agarose ,Autologous transplantation ,Antibody - Abstract
To disclose the influence of foreign body responses raised against a non-absorbable hydrogel consisting of tissue-engineered cartilage, we embedded human/canine chondrocytes within agarose and transplanted them into subcutaneous pockets in nude mice and donor beagles. One month after transplantation, cartilage formation was observed in the experiments using human chondrocytes in nude mice. No significant invasion of blood cells was noted in the areas where the cartilage was newly formed. Around the tissue-engineered cartilage, agarose fragments, a dense fibrous connective tissue and many macrophages were observed. On the other hand, no cartilage tissue was detected in the autologous transplantation of canine chondrocytes. Few surviving chondrocytes were observed in the agarose and no accumulation of blood cells was observed in the inner parts of the transplants. Localizations of IgG and complements were noted in areas of agarose, and also in the devitalized cells embedded within the agarose. Even if we had inhibited the proximity of the blood cells to the transplanted cells, the survival of the cells could not be secured. We suggest that these cytotoxic mechanisms seem to be associated not only with macrophages but also with soluble factors, including antibodies and complements.
- Published
- 2011
5. Feasibility and limitations of the round robin test for assessment of in vitro chondrogenesis evaluation protocol in a tissue-engineered medical product
- Author
-
Koji Hattori, Tsuyoshi Takato, Takashi Ushida, Masako Yokoi, Kazuto Hoshi, Yukio Kato, Akira Myoui, Tomoko Kobo, Katsura Sugawara, Mika Tadokoro, Hajime Ohgushi, Katsuhiko Nanno, Koichi Narikawa, and Masami Kanawa
- Subjects
Biomaterials ,Protocol (science) ,Engineering ,Tissue engineered ,business.industry ,Medical product ,Biomedical Engineering ,Medicine (miscellaneous) ,Round robin test ,business ,Biomedical engineering - Abstract
Tissue-engineered medical products (TEMPs) should be evaluated before implantation. Therefore, it is indispensable to establish evaluation protocols in regenerative medicine. Whether or not such evaluation protocols are reasonable is generally verified through a 'round robin' test. However, the round robin test for TEMPs intrinsically includes a deficiency, because 'identical' specimens can not be prepared for TEMPs. The aim of the study was to assess the feasibility and limitations of the round robin test for TEMPs by using a prepared evaluation protocol. We adopted tissue-engineered cartilage constructs as delivered specimens and a protocol of measuring sGAG content as an evaluation protocol proposed to ISO TC150/SC7, which is an invasive, but usually applied, method, although non-invasive methods are keenly required in evaluating TEMPs. The results showed that: (a) the coefficient of variation (CV) of the measured sGAG contents in intralaboratory tests was ~5% at most; (b) the CV of sGAG content in the scheme where each participating laboratory measured different constructs was comparable with that in the scheme where each participating laboratory measured one half of a construct along with the organizing laboratory; (c) the CV caused by factors other than the specimen was ~15%, comparable to that in reproducible experiments in biomedical fields. Based on these results, the study concludes that a round robin test for a TEMP could be valuable, under the condition that the delivered TEMPs are sufficiently reproducible so that the CV of the measured values is
- Published
- 2011
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.