1. Gemifloxacin, a Fluoroquinolone Antimicrobial Drug, Inhibits Migration and Invasion of Human Colon Cancer Cells
- Author
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Jung-Yu Kan, Ya-Ling Hsu, Jaw-Yuan Wang, Tun-Chieh Chen, Po-Lin Kuo, and Yen-Hsu Chen
- Subjects
Epithelial-Mesenchymal Transition ,Article Subject ,Gemifloxacin ,Colorectal cancer ,lcsh:Medicine ,Biology ,General Biochemistry, Genetics and Molecular Biology ,Metastasis ,Anti-Infective Agents ,Cell Movement ,Cell Line, Tumor ,Biomarkers, Tumor ,medicine ,Humans ,Neoplasm Invasiveness ,Epithelial–mesenchymal transition ,Naphthyridines ,Cell Nucleus ,Inflammation ,General Immunology and Microbiology ,Tumor Necrosis Factor-alpha ,lcsh:R ,NF-kappa B ,Cell migration ,General Medicine ,medicine.disease ,NFKB1 ,Protein Transport ,Cell culture ,Colonic Neoplasms ,Immunology ,Cancer research ,Tumor necrosis factor alpha ,Snail Family Transcription Factors ,Drug Screening Assays, Antitumor ,Fluoroquinolones ,Transcription Factors ,Research Article ,medicine.drug - Abstract
Gemifloxacin (GMF) is an orally administered broad-spectrum fluoroquinolone antimicrobial agent used to treat acute bacterial exacerbation of pneumonia and bronchitis. Although fluoroquinolone antibiotics have also been found to have anti-inflammatory and anticancer effects, studies on the effect of GMF on treating colon cancer have been relatively rare. To the best of our knowledge, this is the first report to describe the antimetastasis activities of GMF in colon cancer and the possible mechanisms involved. Results have shown that GMF inhibits the migration and invasion of colon cancer SW620 and LoVo cells and causes epithelial mesenchymal transition (EMT). In addition, GMF suppresses the activation of NF-κB and cell migration and invasion induced by TNF-αand inhibits the TAK1/TAB2 interaction, resulting in decreased IκB phosphorylation and NF-κB nuclear translocation in SW620 cells. Furthermore, Snail, a critical transcriptional factor of EMT, was downregulated after GMF treatment. Overexpression of Snail by cDNA transfection significantly decreases the inhibitory effect of GMF on EMT and cell migration and invasion. In conclusion, GMF may be a novel anticancer agent for the treatment of metastasis in colon cancer.
- Published
- 2013