1. Protein Profiling of B-Cell Lymphomas Using Tissue Biopsies: A Potential Tool for Small Samples in Pathology
- Author
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Corine Jansen, Konnie M. Hebeda, Marianne Linkels, Johanna M. M. Grefte, John M. M. Raemaekers, Johan H. J. M. van Krieken, and Patricia J. T. A. Groenen
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Non-Hodgkin’s lymphoma comprises many related but distinct diseases and diagnosis and classification is complex. Protein profiling of lymphoma biopsies may be of potential value for use in this lymphoma classification and the discovery of novel markers. In this study, we have optimized a method for SELDI-TOF MS based protein profiling of frozen tissue sections, without dissection of tumour cells. First we have compared chip surfaces and lysis buffers. Also, we have determined the minimal input using laser dissection microscopy. Subsequently, we have analyzed and compared protein profiles of diffuse large B-cell lymphoma (n=8), follicular lymphoma (n=8) and mantle cell lymphoma (n=8). Benign, reactive lymph nodes (n=14) were used as a reference group.
- Published
- 2008
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