1. Effects of Bufei Yishen Granules Combined with Acupoint Sticking Therapy on Pulmonary Surfactant Proteins in Chronic Obstructive Pulmonary Disease Rats
- Author
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Lili Wang, Fengjia Yao, Yuqiong Dong, Linlin Li, Yange Tian, Jing Mao, Jiansheng Li, Ming-hang Wang, Shan Luo, and Ya Li
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,Pulmonary Surfactant-Associated Proteins ,Article Subject ,Acupuncture Therapy ,Pulmonary disease ,lcsh:Medicine ,Pharmacology ,General Biochemistry, Genetics and Molecular Biology ,Rats, Sprague-Dawley ,03 medical and health sciences ,Pulmonary Disease, Chronic Obstructive ,0302 clinical medicine ,Pulmonary surfactant ,Medicine ,Animals ,RNA, Messenger ,EF50 ,Lung ,Bufei yishen ,COPD ,General Immunology and Microbiology ,business.industry ,lcsh:R ,General Medicine ,medicine.disease ,Combined Modality Therapy ,Pathophysiology ,Surgery ,Rats ,Respiratory Function Tests ,respiratory tract diseases ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Aminophylline ,Female ,business ,Acupuncture Points ,Bronchoalveolar Lavage Fluid ,medicine.drug ,Drugs, Chinese Herbal ,Research Article - Abstract
Our previous studies have demonstrated the beneficial effects of Bufei Yishen granules combined with acupoint sticking therapy (the integrated therapy) in chronic obstructive pulmonary disease (COPD), but the underlying mechanism remains unclear. Dysfunction of pulmonary surfactant proteins (SPs, including SP-A, SP-B, SP-C, and SP-D) may be included in pathophysiology of COPD. This study aimed to explore the mechanism of the integrated therapy on SPs. COPD rat models were established. The treatment groups received Bufei Yishen granules or acupoint sticking or their combination. Using aminophylline as a positive control drug. The levels of SPs in serum, BALF, and lung were measured. The results showed that the integrated therapy markedly reduced the levels of SPs in serum and increased these indicators in the lung. The integrated therapy was better than aminophylline in reducing the levels of SPs and was better than Bufei Yishen granules in reducing SP-A, SP-C, and SP-D in serum. The integrated therapy was better than aminophylline and Bufei Yishen granules in increasing SP-A, SP-B, and SP-D mRNA in the lung. SP-A and SP-D in BALF were positively correlated with PEF and EF50. The levels of SPs are associated with airway limitation. The beneficial effects of the integrated therapy may be involved in regulating pulmonary surfactant proteins.
- Published
- 2016