1. Characterization of NO-Induced Nitrosative Status in Human Placenta from Pregnant Women with Gestational Diabetes Mellitus
- Author
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Rosa M. Mateos-Bernal, M. del Mar Gil-Sánchez, Fernando Bugatto, Francisco Visiedo, Cristina López-Tinoco, Manuel Aguilar-Diosdado, Celeste Santos-Rosendo, Carmen Segundo, Universidad de Sevilla. Departamento de Genética, Ministerio de Educación y Ciencia (MEC). España, Junta de Andalucía, [Visiedo, Francisco] Puerta Mar Univ Hosp, Res Unit, Cadiz, Spain, [Santos-Rosendo, Celeste] Puerta Mar Univ Hosp, Res Unit, Cadiz, Spain, [Mateos-Bernal, Rosa M.] Puerta Mar Univ Hosp, Res Unit, Cadiz, Spain, [del Mar Gil-Sanchez, M.] Univ Seville, Fac Biol, Dept Genet, Seville, Spain, [Bugatto, Fernando] Puerta Mar Univ Hosp, Dept Obstet & Gynecol, Cadiz, Spain, [Lopez-Tinoco, Cristina] Puerta Mar Univ Hosp, Dept Obstet & Gynecol, Cadiz, Spain, [Aguilar-Diosdado, Manuel] Puerta Mar Univ Hosp, Dept Endocrinol & Nutr, Cadiz, Spain, [Segundo, Carmen] Univ Cadiz, Fac Nursing, Salus Infirmorum, Cadiz, Spain, Andalusia Department of Health, and PAIDI
- Subjects
0301 basic medicine ,Aging ,endocrine system diseases ,Placenta ,Nitric Oxide Synthase Type II ,Expression ,Apoptosis ,Biochemistry ,Body Mass Index ,0302 clinical medicine ,Nitric-oxide ,Pregnancy ,Mitogen-Activated Protein Kinase 1 ,Mitogen-Activated Protein Kinase 3 ,biology ,Caspase 3 ,lcsh:Cytology ,Nitrosylation ,P38 ,General Medicine ,Catalase ,Caspase 9 ,Trophoblasts ,Gestational diabetes ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Female ,Research Article ,Adult ,medicine.medical_specialty ,Article Subject ,Nitrosation ,Nitric Oxide ,Pathophysiology ,03 medical and health sciences ,Erk1/2 ,Internal medicine ,medicine ,Humans ,lcsh:QH573-671 ,Protein kinase B ,Fetus ,Nitrates ,S-Nitrosothiols ,Cesarean Section ,Cell Biology ,Peroxiredoxins ,medicine.disease ,Protein s-nitrosylation ,Diabetes, Gestational ,030104 developmental biology ,Endocrinology ,Oxidative stress ,Case-Control Studies ,biology.protein ,Peroxiredoxin ,Proto-Oncogene Proteins c-akt - Abstract
Dysregulation of NO production is implicated in pregnancy-related diseases, including gestational diabetes mellitus (GDM). The role of NO and its placental targets in GDM pregnancies has yet to be determined. S-Nitrosylation is the NO-derived posttranslational protein modification that can modulate biological functions by forming NO-derived complexes with longer half-life, termed S-nitrosothiol (SNO). Our aim was to examine the presence of endogenous S-nitrosylated proteins in cysteine residues in relation to antioxidant defense, apoptosis, and cellular signal transduction in placental tissue from control (n=8) and GDM (n=8) pregnancies. S-Nitrosylation was measured using the biotin-switch assay, while the expression and protein activity were assessed by immunoblotting and colorimetric methods, respectively. Results indicated that catalase and peroxiredoxin nitrosylation levels were greater in GDM placentas, and that was accompanied by reduced catalase activity. S-Nitrosylation of ERK1/2 and AKT was increased in GDM placentas, and their activities were inhibited. Activities of caspase-3 and caspase-9 were increased, with the latter also showing diminished nitrosylation levels. These findings suggest that S-nitrosylation is a little-known, but critical, mechanism by which NO directly modulates key placental proteins in women with GDM and, as a consequence, maternal and fetal anomalies during pregnancy can occur.
- Published
- 2017