Search

Your search keyword '"Calábria, Luciana Karen"' showing total 4 results
Start Over Author "Calábria, Luciana Karen" Publisher hindawi limited
4 results on '"Calábria, Luciana Karen"'

2. Myosins Are Differentially Expressed under Oxidative Stress in Chronic Streptozotocin-Induced Diabetic Rat Brains

Author

Calábria, Luciana Karen, primary, Vieira da Costa, Alice, additional, da Silva Oliveira, Renato José, additional, Ramos Deconte, Simone, additional, Nascimento, Rafael, additional, de Carvalho, Washington João, additional, de Oliveira, Vanessa Neves, additional, Arcaro Filho, Carlos Alberto, additional, Rezende Alves de Oliveira, Luciana, additional, Goulart, Luiz Ricardo, additional, and Espindola, Foued Salmen, additional

Published
2013
Full Text
View/download PDF

3. Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa.

Author

Moraes, Izabela Barbosa, Manzan-Martins, Camilla, de Gouveia, Neire Moura, Calábria, Luciana Karen, Hiraki, Karen Renata Nakamura, Moraes, Alberto da Silva, and Espindola, Foued Salmen

Subjects
*DIAGNOSIS of diabetes, *ANIMAL experimentation, *CHROMOSOME abnormalities, *LIVER, *RATS, *PLANT extracts, *OXIDATIVE stress
Abstract

Diabetes mellitus (DM) is characterized by hyperglycemia and alterations in the metabolism of lipids, carbohydrates, and proteins. Due to its hypoglycemic effect Vochysia rufa is frequently used in Uberlandia, Brazil, to treat DM. Despite its popularity, there is little information about its effect on hepatic tissue. Therefore, we evaluated the histoarchitecture, oxidative stress parameters, and polyploidy of liver tissue from streptozotocin- (STZ-) induced diabetic rats treated with aqueous extract of Vochysia rufa (AEV). Histology was determined by fixing the livers, processing, and staining with HE. Oxidative stress was determined by evaluating CAT, GPx, and SOD activity in liver homogenates and hepatic mitochondria fraction and by measuring GST, GSH levels and lipid peroxidation (MDA). Polyploidy was determined by subjecting isolated hepatocyte nuclei to flow cytometry. In the diabetic group, GST activity and GSH rates decreased whereas liver homogenate analysis showed that GPx, SOD activity and MDA increased. AEV treatment restored all parameters to normal levels. The oxidative stress analysis of hepatic mitochondria fraction showed similar results. Lower polyploid cell populations were found in the diabetic rat livers, even after glibenclamide treatment. Thus, AEV treatment efficiently reduced hepatic oxidative stress caused by STZ-induced diabetes and produced no morphological changes in the histological analysis. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

4. Polyploidy Analysis and Attenuation of Oxidative Stress in Hepatic Tissue of STZ-Induced Diabetic Rats Treated with an Aqueous Extract of Vochysia rufa.

Author

Barbosa Moraes, Izabela, Manzan-Martins, Camilla, de Gouveia, Neire Moura, Calábria, Luciana Karen, Nakamura Hiraki, Karen Renata, da Silva Moraes, Alberto, and Espindola, Foued Salmen

Subjects
*BLOOD sugar analysis, *DIABETES prevention, *GENES, *LIVER analysis, *ENZYME metabolism, *ALTERNATIVE medicine, *ANIMAL experimentation, *BARK, *BIOPHYSICS, *BODY weight, *FLOW cytometry, *GLUTATHIONE, *HISTOLOGICAL techniques, *HYPOGLYCEMIC agents, *LIVER, *RESEARCH methodology, *MEDICINAL plants, *LIPID peroxidation (Biology), *MITOCHONDRIA, *PROBABILITY theory, *RATS, *RESEARCH funding, *STAINS & staining (Microscopy), *PLANT stems, *SUPEROXIDE dismutase, *T-test (Statistics), *PLANT extracts, *PILOT projects, *OXIDATIVE stress, *DATA analysis software, *DESCRIPTIVE statistics, *PHARMACODYNAMICS
Abstract

Diabetes mellitus (DM) is characterized by hyperglycemia and alterations in the metabolism of lipids, carbohydrates, and proteins. Due to its hypoglycemic effect Vochysia rufa is frequently used in Uberlandia, Brazil, to treat DM. Despite its popularity, there is little information about its effect on hepatic tissue. Therefore, we evaluated the histoarchitecture, oxidative stress parameters, and polyploidy of liver tissue from streptozotocin- (STZ-) induced diabetic rats treated with aqueous extract of Vochysia rufa (AEV). Histology was determined by fixing the livers, processing, and staining with HE. Oxidative stress was determined by evaluating CAT, GPx, and SOD activity in liver homogenates and hepatic mitochondria fraction and by measuring GST, GSH levels and lipid peroxidation (MDA). Polyploidy was determined by subjecting isolated hepatocyte nuclei to flow cytometry. In the diabetic group, GST activity and GSH rates decreased whereas liver homogenate analysis showed that GPx, SOD activity and MDA increased. AEV treatment restored all parameters to normal levels. The oxidative stress analysis of hepatic mitochondria fraction showed similar results. Lower polyploid cell populations were found in the diabetic rat livers, even after glibenclamide treatment. Thus, AEV treatment efficiently reduced hepatic oxidative stress caused by STZ-induced diabetes and produced no morphological changes in the histological analysis. [ABSTRACT FROM AUTHOR]

Published
2015
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results