1. Detection and Quantification of Fluorescent Cell Clusters in Cryo-Imaging.
- Author
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Steyer, Grant J., Feng Dong, Kanodia, Lehar, Roy, Debashish, Penn, Marc, and Wilson, David L.
- Subjects
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THERAPEUTICS , *ALGORITHMS , *ANIMAL experimentation , *MICE , *RESEARCH funding , *STEM cells , *EQUIPMENT & supplies , *DICOM (Computer network protocol) - Abstract
We developed and evaluated an algorithm for enumerating fluorescently labeled cells (e.g., stem and cancer cells) in mouse-sized, microscopic-resolution, cryo-image volumes. Fluorescent cell clusters were detected, segmented, and then fit with a model which incorporated a priori information about cell size, shape, and intensity. The robust algorithm performed well in phantom and tissue imaging tests, including accurate (<2% error) counting of cells in mouse. Preliminary experiments demonstrate that cryo-imaging and software can uniquely analyze delivery, homing to an organ and tissue distribution of stem cell therapeutics. [ABSTRACT FROM AUTHOR]
- Published
- 2012
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