1. Danggui Shaoyao San Ameliorates Renal Fibrosis via Regulation of Hypoxia and Autophagy
- Author
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Hui-juan Chen, Ling-ling Zhu, Huihua Chen, Ting Zhang, Pei Zhao, Jing Tian, and Meng-yun Zhang
- Subjects
0301 basic medicine ,Article Subject ,Renal Hypertrophy ,Renal function ,Benazepril ,Pharmacology ,Immunofluorescence ,urologic and male genital diseases ,digestive system ,03 medical and health sciences ,0302 clinical medicine ,Western blot ,medicine ,Renal fibrosis ,medicine.diagnostic_test ,business.industry ,urogenital system ,Autophagy ,lcsh:Other systems of medicine ,Hypoxia (medical) ,lcsh:RZ201-999 ,female genital diseases and pregnancy complications ,stomatognathic diseases ,030104 developmental biology ,Complementary and alternative medicine ,medicine.symptom ,business ,030217 neurology & neurosurgery ,medicine.drug ,Research Article - Abstract
Danggui Shaoyao San (DSS), a traditional Chinese medicinal prescription, was widely used to reinforce earth to activate collaterals in ancient times. Recently, many clinical studies found that DSS had a renoprotection. In this study, we evaluated the effect of DSS on unilateral ureteral obstruction- (UUO-) induced renal fibrosis in rats and investigated the mechanisms underlying the effect. Sprague Dawley (SD) rats were randomized to UUO or Sham operation. After 1 day, the rats that underwent UUO were randomized to treatment for four experimental groups (n=10 each group): Sham, UUO only, UUO+ benazepril (Bena), and UUO+DSS. After 4 weeks, we demonstrated that DSS significantly suppressed UUO-induced renal hypertrophy by gravimetric. In addition, DSS obviously prevented UUO-induced disorder in renal structure and renal function by HE and biochemistry test. We also found that DSS abrogated UUO-induced renal fibrosis by Masson’s staining and collagen volume fraction (CVF) analysis; this is consistent with the western blot analysis that showed DSS abrogated the UUO-induced enhanced TGF-β1 and weakened BMP-7. Compared with the UUO only group, rats treated with DSS exhibited significant increase in vascular density, followed by decrease in hypoxia and HIF-1α protein level through western blot and immunofluorescence analysis. Furthermore, we also determined proteins of autophagy and DSS enhanced autophagy to prevent the damage-induced by UUO. Taken together, our findings demonstrated that DSS had a renoprotection effect in ameliorating renal fibrosis possibly via attenuating tissue hypoxia and regulating autophagy.
- Published
- 2019