1. Transition from vitreous to floury endosperm in maize (Zea mays L.) kernels is related to protein and starch gradients
- Author
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Laurent Linossier, Cédric Gaillard, Caroline Delluc, Bénédicte Bakan, Sylvie Brunet, Didier Marion, Mathieu Gayral, Khalil Elmorjani, Marie-Hélène Morel, Michèle Dalgalarrondo, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Groupe Limagrain, Limagrain Cereales Ingredients, Ingénierie des Agro-polymères et Technologies Émergentes (UMR IATE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Fond unique interministériel FUI (GranoFlakes, F1204004C), Limagrain Europe, and Fond unique interministeriel (FUI GranoFlakes) : F1204004C.
- Subjects
0106 biological sciences ,genetic structures ,Starch ,développement de l'endosperme ,Starch channels ,01 natural sciences ,Biochemistry ,teneur en amylose ,Endosperm ,Protein content ,zea mays ,chemistry.chemical_compound ,Zein gradient ,0404 agricultural biotechnology ,Amylose ,[SDV.IDA]Life Sciences [q-bio]/Food engineering ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Vitreousness ,flint maize ,2. Zero hunger ,endosperme ,indian corn ,maize starch ,digestive, oral, and skin physiology ,A protein ,food and beverages ,04 agricultural and veterinary sciences ,microspectroscopie ,Maize endosperm ,040401 food science ,maïs vitreux ,Zea mays ,eye diseases ,Raman microspectroscopy ,chemistry ,Biophysics ,amidon de mais ,Starch granule ,sense organs ,seed albumins ,010606 plant biology & botany ,Food Science - Abstract
International audience; Relationships between kernel vitreousness and proteins and starch partitioning to the floury and vitreous regions of the endosperm were monitored in a set of 13 maize inbred lines. Decrease of protein contents from the vitreous to the floury endosperms were mainly assigned to a-zeins. Using Raman microspectroscopy, we observed a protein gradient from the periphery to the center of endosperms that well fitted with the inverse relationships between vitreousness and protein content of the vitreous and floury regions. In addition, Raman microspectroscopy highlighted an increase of starch crystallinity from the periphery to the center of the maize endosperms. This agrees with the higher amylose and associated lipid contents within starches of vitreous than in those of floury endosperms. Finally, starch granules from vitreous regions displayed more channels than the floury ones. These channels contain proteins that might favor adhesion of proteins to starch granules or granule granule contacts to form the close packing of the vitreous endosperm. Therefore transition from vitreous to floury endosperm is at least the result of both protein and starch gradients. These gradients are probably associated with metabolic gradients that have been observed during endosperm development. (C) 2016 Elsevier Ltd. All rights reserved.
- Published
- 2016
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