1. Dynamics of detachment of Escherichia coli from plasma-mediated coatings under shear flow
- Author
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Patrice Raynaud, Christophe Ellero, Nathalie Lebleu, Philippe Schmitz, Muriel Mercier-Bonin, Marie-Pierre Duviau, Bernard Despax, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées, 'BioPleasure' project of the Research National Agency [ANR-07-BLAN-0196-01], Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), LAboratoire PLasma et Conversion d'Energie (LAPLACE), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), Matériaux et Procédés Plasmas (LAPLACE-MPP), Université de Toulouse (UT)-Université Toulouse III - Paul Sabatier (UT3), ANR-07-BLAN-0196,BIOPLEASURE,Plasma - surface engineering for biofilm prevention(2007), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), and Université Fédérale Toulouse Midi-Pyrénées-Université Toulouse III - Paul Sabatier (UT3)
- Subjects
Silver ,Materials science ,shear flow ,Surface Properties ,[SDV]Life Sciences [q-bio] ,Population ,attachment phenotype ,surface chemistry ,02 engineering and technology ,Aquatic Science ,engineering.material ,Matrix (biology) ,YEAST SACCHAROMYCES-CEREVISIAE ,SILVER NANOPARTICLES ,ADHESION ,Applied Microbiology and Biotechnology ,Bacterial Adhesion ,Silver nanoparticle ,Hydrophobic effect ,03 medical and health sciences ,THIN-FILMS ,Coating ,Escherichia coli ,Shear stress ,anti-adhesive properties ,DEPOSITION ,education ,Water Science and Technology ,0303 health sciences ,education.field_of_study ,030306 microbiology ,SURFACES ,E. coli ,PSEUDOMONAS-AERUGINOSA ,Adhesion ,ANTIMICROBIAL PROPERTIES ,Stainless Steel ,021001 nanoscience & nanotechnology ,STAINLESS-STEEL ,plasma-mediated coatings ,engineering ,Biophysics ,Shear Strength ,0210 nano-technology ,Shear flow ,CHAMBER - Abstract
A series of plasma-mediated coatings, containing silver nanoparticles embedded in an organosilicon or silica-like matrix, were deposited onto stainless steel and chemically characterized. Their anti-adhesive properties were evaluated in vitro towards Escherichia coli by performing shear-flow induced detachment experiments. Increasing the wall shear stress facilitated E. coli cell detachment, irrespective of the coating characteristics. When nanosilver was incorporated, cell detachment was lower, probably due to the affinity of the embedded silver for biological components of the cell wall. The presence of methyl groups in the matrix network could also promote enhanced hydrophobic interactions. Within the population fraction remaining attached to the coating under increasing shear flow, different association phenotypes were observed, viz. progressively lying flat, moving laterally, remaining tethered, or rotating by a single anchoring point, until alignment with the flow direction. This re-orientation phenotype and its relation with detachment were dependent of the coating. The effects of such heterogeneities should be more deeply explored.
- Published
- 2012