1. Proteomic expression profile of injured rat peripheral nerves revealed biological networks and processes associated with nerve regeneration
- Author
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Alessandro Sannino, Stefania De Domenico, Michel Salzet, Isabelle Fournier, Anna Maria Giudetti, Velia La Pesa, Alessandro Romano, Ilaria Cicalini, Eleonora Stanca, Daniele Vergara, Angelo Quattrini, Damiana Pieragostino, Federica Cerri, Elisa Storelli, Laura Aloisi, Michele Maffia, Julien Franck, University of Salento [Lecce], Liceo Classico Giovanni Paolo II [Lecce LE, Italie], IRCCS Ospedale San Raffaele [Milan, Italy], Institute of Food Production Sciences [Lecce, Italy], Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), University 'G. d'Annunzio' of Chieti-Pescara [Chieti], Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), SALZET, Michel, Vergara, D, Romano, A, Stanca, E, La Pesa, V, Aloisi, Al, De Domenico, S, Franck, J, Cicalini, I, Giudetti, Am, Storelli, E, Pieragostino, D, Fournier, I, Sannino, A, Salzet, M, Cerri, F, Quattrini, A, and Maffia, M
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0301 basic medicine ,Physiology ,[SDV]Life Sciences [q-bio] ,Clinical Biochemistry ,Proteomics ,DGAT ,Rats, Sprague-Dawley ,03 medical and health sciences ,0302 clinical medicine ,proteomics ,Tandem Mass Spectrometry ,lipid metabolism ,medicine ,Animals ,peripheral nerve injury ,Diacylglycerol O-Acyltransferase ,LC-MS/MS ,Cytoskeleton ,Chemistry ,Regeneration (biology) ,Computational Biology ,Lipid metabolism ,Cell Biology ,Metabolism ,Nerve injury ,Sciatic Nerve ,Cell biology ,Nerve Regeneration ,Rats ,[SDV] Life Sciences [q-bio] ,030104 developmental biology ,Peripheral nerve injury ,Female ,Sciatic nerve ,Schwann Cells ,medicine.symptom ,Nervous System Diseases ,030217 neurology & neurosurgery ,Chromatography, Liquid ,Signal Transduction - Abstract
International audience; Peripheral nerve regeneration is regulated through the coordinated spatio-temporal activation of multiple cellular pathways. In this work, an integrated proteomics and bioinformatics approach was employed to identify differentially expressed proteins at the injury-site of rat sciatic nerve at 20 days after damage. By a label-free liquid chromatography mass-spectrometry (LC-MS/MS) approach, we identified 201 differentially proteins that were assigned to specific canonical and disease and function pathways. These include proteins involved in cytoskeleton signaling and remodeling, acute phase response, and cellular metabolism. Metabolic proteins were significantly modulated after nerve injury to support a specific metabolic demand. In particular, we identified a group of proteins involved in lipid uptake and lipid storage metabolism. Immunofluorescent staining for acyl-CoA diacylglycerol acyltransferase 1 (DGAT1) and DAGT2 expression provided evidence for the expression and localization of these two isoforms in Schwann cells at the injury site in the sciatic nerve. This further supports a specific local regulation of lipid metabolism in peripheral nerve after damage.
- Published
- 2018
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