1. A New Method for Visualizing Drusen and Their Progression in Flood-Illumination Adaptive Optics Ophthalmoscopy
- Author
-
Ethan A. Rossi, Nathaniel Norberg, Chiara Eandi, Celine Chaumette, Saloni Kapoor, Laura Le, Valerie C. Snyder, Joseph N. Martel, Josselin Gautier, Kiyoko Gocho, Kunal K. Dansingani, Jay Chhablani, Angelo Arleo, Sarah Mrejen, José-Alain Sahel, Kate Grieve, Michel Paques, Gestionnaire, Hal Sorbonne Université, University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE), McGowan Institute for Regenerative Medicine, University of Pittsburgh (PITT), Pennsylvania Commonwealth System of Higher Education (PCSHE)-Pennsylvania Commonwealth System of Higher Education (PCSHE), Centre Hospitalier National d'Ophtalmologie des Quinze-Vingts (CHNO), Institut de la Vision, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), and Università degli studi di Torino = University of Turin (UNITO)
- Subjects
[SDV.IB] Life Sciences [q-bio]/Bioengineering ,Horizon 2020 ,genetic structures ,Biomedical Engineering ,drusen ,Retinal Drusen ,MACUSTAR ,Floods ,Article ,eye diseases ,adaptive optics ,Ophthalmoscopy ,Ophthalmology ,age-related macular degeneration ,image analysis ,[SDV.MHEP.OS] Life Sciences [q-bio]/Human health and pathology/Sensory Organs ,Humans ,[SDV.IB]Life Sciences [q-bio]/Bioengineering ,sense organs ,Fluorescein Angiography ,European Union (EU) ,[SDV.MHEP.OS]Life Sciences [q-bio]/Human health and pathology/Sensory Organs ,Lighting - Abstract
Purpose: Drusen are dynamic sub-RPE deposits that are risk factors for late-stage agerelated macular degeneration (AMD). Here we show a new imaging method using floodillumination adaptive optics (FIAO) that reveal drusen with high contrast and resolution. Methods: A fovea-centered 4° × 4° FIAO image and eight surrounding images with gaze displaced by ±2° vertically and horizontally were acquired. Clinical color fundus and spectral-domain optical coherence tomography were acquired for clinical grading and comparison. Custom software registered overlapping FIAO images and fused the data statistically to generate a fovea-centered 4° × 4° gaze-dependent image. Our dataset included 15 controls (aged 31–72) and 182 eyes from 104 AMD patients (aged 56–92), graded as either normal aging (n = 7), and early (n = 12), intermediate (n = 108) and late AMD (n = 42); 27 had subretinal drusenoid deposits (SDDs), and 83 were imaged longitudinally. Results: No gaze varying structures were detected in young eyes. In aging eyes with no evidence of age-related changes, putative drusen Translational Relevance: This new technique offers promise as a robust and sensitive method to detect, map, quantify, and monitor the dynamics of drusen in aging and AMD.
- Published
- 2021
- Full Text
- View/download PDF