1. The activity of three glycosidases (β-Ν-acetyloglucosaminidase, α-mannosidase, and β-galactosidase) in the follicular fluid and in the maturation medium affects bovine oocyte maturation
- Author
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F. Krania, Pascal Mermillod, E. Vainas (Ε. Βαϊνασ), Theodora Tsiligianni, Eleni Dovolou, Christine Perreau, F. Samartzi, Amanda Cordova, Georgios S. Amiridis, Department of Obstetrics & Reproduction, Faculty of Veterinary Medicine, University of Thessaly [Volos] (UTH), Veterinary Research Institute of Thessaloniki, National Agricultural Research Foundation (NAGREF), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), GSRT and ERDF (Greece, grand number 09FR73), French Ministry of Foreign Affairs (France, grand number 23442XA), National Agricultural Research Foundation, Centre National de la Recherche Scientifique (CNRS)-Université de Tours-Institut Français du Cheval et de l'Equitation [Saumur]-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Mannosidase ,medicine.medical_specialty ,[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT] ,In Vitro Oocyte Maturation Techniques ,Cell Culture Techniques ,Biology ,alpha-Mannosidase ,follicle ,03 medical and health sciences ,Follicle ,0302 clinical medicine ,Food Animals ,Internal medicine ,Acetylglucosaminidase ,Follicular phase ,medicine ,Animals ,Small Animals ,Incubation ,030219 obstetrics & reproductive medicine ,Equine ,maturation ,0402 animal and dairy science ,cumulus oocyte complex ,04 agricultural and veterinary sciences ,beta-Galactosidase ,Oocyte ,040201 dairy & animal science ,Follicular fluid ,Culture Media ,Follicular Fluid ,Endocrinology ,medicine.anatomical_structure ,glycosidase ,Oocytes ,Cattle ,Female ,Animal Science and Zoology - Abstract
We studied the role of follicular fluid's (FF) glycosidase (α-mannosidase [α-ΜΑΝ], β-Ν-acetyloglucosaminidase [NAGASE], β-galactosidase [β-GAL]) activity during IVM of bovine oocytes. Oocytes were allocated into two groups according to the follicular size (small follicle [SF]: 2–5 mm, large follicle [LF]: >5–8 mm). In experiment 1, cumulus–oocyte complexes (COCs) quality was evaluated according to morphologic criteria (grades A, B–C, D); oocyte (n = 801) nuclear maturation was assessed after 24 hours of incubation. Bovine embryos were produced in vitro in groups (experiment 2, n = 1503 oocytes) or individually (experiment 3, n = 50 oocytes). More grade-A and -BC COCs were collected from SF and LF groups, respectively (P
- Published
- 2016