1. Gene expression profiling in sinonasal adenocarcinoma
- Author
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Olivier Malard, Isabelle Guisle-Marsollier, Sylvia Quemener, Karine Renaudin, C. Ferron, Véronique Sébille-Rivain, Dominique Tripodi, Christian Verger, Christian Geraut, Catherine Gratas-Rabbia-Ré, Service de Médecine du Travail et des Risques Professionnels, Centre de Recherche en Cancérologie Nantes-Angers (CRCNA), Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM)-Hôtel-Dieu de Nantes-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Laennec-Centre National de la Recherche Scientifique (CNRS)-Faculté de Médecine d'Angers-Centre hospitalier universitaire de Nantes (CHU Nantes), Service d'Anatomie Pathologique, EA Biométadys, Université de Nantes (UN), Service ORL, Plateforme Puces à ADN-OGP, Laboratoire de Biomathématiques-Biostatistiques, Consultation des Pathologies Professionnelles, CH Hôtel-Dieu, Service de Biochimie, Grant support: La Ligue Contre le Cancer, comité Pays de la LOIRE, La Direction de la Recherche Clinique du Centre Hospitalo-Universitaire de Nantes., and BMC, Ed.
- Subjects
Male ,MESH: Neoplasm Proteins ,Microarray ,Galectin 4 ,0302 clinical medicine ,Ethmoid Sinus ,Gene expression ,Genetics(clinical) ,MESH: Ethmoid Sinus ,Genetics (clinical) ,MESH: Coenzyme A Ligases ,Oligonucleotide Array Sequence Analysis ,MESH: Aged ,0303 health sciences ,MESH: Middle Aged ,biology ,Middle Aged ,Immunohistochemistry ,3. Good health ,Neoplasm Proteins ,030220 oncology & carcinogenesis ,Adenocarcinoma ,[SDV.BBM.GTP] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Female ,DNA microarray ,Paranasal Sinus Neoplasms ,lcsh:Internal medicine ,lcsh:QH426-470 ,03 medical and health sciences ,MESH: Gene Expression Profiling ,[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN] ,Research article ,Coenzyme A Ligases ,medicine ,Genetics ,Humans ,lcsh:RC31-1245 ,Gene ,030304 developmental biology ,Aged ,MESH: Paranasal Sinus Neoplasms ,MESH: Humans ,Clusterin ,Gene Expression Profiling ,MESH: Adenocarcinoma ,MESH: Immunohistochemistry ,medicine.disease ,Molecular biology ,MESH: Male ,MESH: Galectin 4 ,Gene expression profiling ,lcsh:Genetics ,MESH: Clusterin ,MESH: Oligonucleotide Array Sequence Analysis ,Cancer research ,biology.protein ,MESH: Female - Abstract
Background Sinonasal adenocarcinomas are uncommon tumors which develop in the ethmoid sinus after exposure to wood dust. Although the etiology of these tumors is well defined, very little is known about their molecular basis and no diagnostic tool exists for their early detection in high-risk workers. Methods To identify genes involved in this disease, we performed gene expression profiling using cancer-dedicated microarrays, on nine matched samples of sinonasal adenocarcinomas and non-tumor sinusal tissue. Microarray results were validated by quantitative RT-PCR and immunohistochemistry on two additional sets of tumors. Results Among the genes with significant differential expression we selected LGALS4, ACS5, CLU, SRI and CCT5 for further exploration. The overexpression of LGALS4, ACS5, SRI, CCT5 and the downregulation of CLU were confirmed by quantitative RT-PCR. Immunohistochemistry was performed for LGALS4 (Galectin 4), ACS5 (Acyl-CoA synthetase) and CLU (Clusterin) proteins: LGALS4 was highly up-regulated, particularly in the most differentiated tumors, while CLU was lost in all tumors. The expression of ACS5, was more heterogeneous and no correlation was observed with the tumor type. Conclusion Within our microarray study in sinonasal adenocarcinoma we identified two proteins, LGALS4 and CLU, that were significantly differentially expressed in tumors compared to normal tissue. A further evaluation on a new set of tissues, including precancerous stages and low grade tumors, is necessary to evaluate the possibility of using them as diagnostic markers.
- Published
- 2009
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