1. A vaccine based on exosomes secreted by a dendritic cell line confers protection against T. gondii infection in syngeneic and allogeneic mice
- Author
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Rachel Guiton, Céline Beauvillain, Sophie Ruiz, Daniel Bout, Isabelle Dimier-Poisson, Inconnu, Immunologie Parasitaire et Vaccinologie, Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT)-UMR 483, and Institut National de la Recherche Agronomique (INRA)-Université de Tours-UMR 483
- Subjects
Protozoan Vaccines ,Adoptive cell transfer ,[SDV]Life Sciences [q-bio] ,Immunology ,Antibodies, Protozoan ,Spleen ,Antigens, Protozoan ,Lymphocyte Activation ,Microbiology ,Exocytosis ,Cell Line ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Immune system ,Antigen ,medicine ,Animals ,Transplantation, Homologous ,ComputingMilieux_MISCELLANEOUS ,030304 developmental biology ,0303 health sciences ,biology ,Cytoplasmic Vesicles ,Dendritic cell ,Dendritic Cells ,Adoptive Transfer ,Microvesicles ,3. Good health ,Transplantation ,Mice, Inbred C57BL ,Transplantation, Isogeneic ,Infectious Diseases ,medicine.anatomical_structure ,Toxoplasmosis, Animal ,030220 oncology & carcinogenesis ,biology.protein ,Mice, Inbred CBA ,Female ,Antibody ,Toxoplasma - Abstract
Our results show that exosomes secreted by SRDC pulsed in vitro with Toxoplasma gondii-derived antigens (Exo-TAg) induced protective responses against infection with the parasite in both syngeneic and allogeneic mice. After oral infection, syngeneic CBA/J mice exhibited significantly fewer cysts in their brains and allogeneic C57BL/6 mice survived. This protection was associated with strong humoral responses in vivo in serum from both CBA/J and C57BL/6 mice, and with high levels of anti-TAg IgA antibodies in intestinal secretions from CBA/J mice alone. Furthermore, strong cellular responses in vivo were observed in both mouse models. Cellular proliferation was associated with cytokines production by spleen and mesenteric lymph node cells. The results presented here show that exosomes are nucleic acid free vesicles that are able to induce immune responses correlated with protection against parasitic infections in both syngeneic and allogeneic mice. They could constitute an efficient tool for use in vaccination and antitumor strategies based on exosomes.
- Published
- 2007
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