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Your search keyword '"Sophie Moreno-Swirc"' showing total 4 results
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4 results on '"Sophie Moreno-Swirc"'

1. In vivo assessment of the pulmonary microcirculation in elastase-induced emphysema using probe-based confocal fluorescence microscopy

Author

Mathieu Salaun, Sophie Moreno-Swirc, Luc Thiberville, Romain Modzelewski, Geneviève Bourg-Heckly, Jean-Paul Marie, Equipe Quantification en Imagerie Fonctionnelle (QuantIF-LITIS), Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Normandie Université (NU), Service de chirurgie, Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU)-Normandie Université (NU), Laboratoire Jean PERRIN, Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Breton, Céline, Université Le Havre Normandie (ULH), Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA), Hôpital Charles Nicolle [Rouen], CHU Rouen, and Normandie Université (NU)-Normandie Université (NU)-CHU Rouen

Subjects
0303 health sciences, Pathology, medicine.medical_specialty, Lung, Confocal, Elastase, respiratory system, Biology, Microcirculation, 03 medical and health sciences, chemistry.chemical_compound, 0302 clinical medicine, medicine.anatomical_structure, 030228 respiratory system, chemistry, In vivo, Fluorescence microscope, medicine, General Earth and Planetary Sciences, Fluorescein, Ex vivo, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, General Environmental Science
Abstract

Introduction: The alveolar capillary bed, which appears essential for the maintenance of alveolar septa, is altered in pulmonary emphysema. Until recently, techniques that allow its analysis in vivo in spontaneously breathing conditions were lacking. Fibered confocal fluorescence microscopy (FCFM) is a new technique that enables distal lung microstructures imaging in vivo. FCFM can be coupled with I.V fluorescein injection to image the pulmonary capillary network. The aim of this study was to assess the lung microcirculation in vivo using FCFM and I.V fluorescein in rats with experimental emphysema.Results: In vivo pulmonary microcirculation imaging was possible in 7/7 elastase animals and in 6/7 controls. Using FCFM, intercapillary distances and alveolar facets diameters were found significantly higher in the elastase group compared with controls (49.5 vs. 41.8 µm p < 0.001, and 118.5 vs. 95.1 µm p < 0.001, respectively). Ex vivo mean interwall distance (MIWD) was correlated with the alveolar facet...

Published
2012

2. Confocal fluorescence endomicroscopy of the human airways

Author

Luc Thiberville, Christine Vever-Bizet, Sophie Moreno-Swirc, Stéphane Dominique, Samy Lachkar, Geneviève Bourg-Heckly, Mathieu Salaün, Service de pneumologie, oncologie thoracique et soins intensifs respiratoires [Rouen], Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-CHU Rouen, Normandie Université (NU), Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire (BIOMOCETI), Université Paris 13 (UP13)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Hôpital Charles Nicolle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), and Breton, Céline

Subjects
Pulmonary and Respiratory Medicine, Materials science, Optical fiber, Confocal, MESH: Microscopy, Fluorescence, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, law.invention, 03 medical and health sciences, 0302 clinical medicine, Optics, Bronchoscopy, Confocal microscopy, law, Microscopy, Fluorescence microscope, medicine, Endomicroscopy, Humans, MESH: Microscopy, Confocal, MESH: Lung, MESH: Bronchoscopy, Lung, Microscopy, Confocal, MESH: Humans, medicine.diagnostic_test, business.industry, 3. Good health, Microscopy, Fluorescence, 030228 respiratory system, 030220 oncology & carcinogenesis, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, business, Preclinical imaging
Abstract

International audience; Confocal endomicroscopes aim at providing to the clinician microscopic imaging of a living tissue. The currently available microendoscopic devices use the principle of confocal fluorescent microscopy, in which the objective is replaced by an optical fiber and a miniaturized scanhead at the distal end of the endoscope or by a retractable bundle of optical fibers. Such systems have recently been applied to the explorations of several organs, including the gastrointestinal tract, and more recently to the proximal and distal airways in vivo. Respiratory fluorescence microendoscopes use 488 nm or 660 nm excitation laser light and thin flexible miniprobes that are introduced into the working channel of the bronchoscope. The devices have a lateral resolution of 3 microm, a field of view of 600 microm, and produce real-time imaging at 9 frames per second. For in vivo imaging, the miniprobe is applied onto the bronchial wall surface or advanced into a distal bronchiole down to the acinus. In nonsmokers, the 488-nm excitation device images the autofluorescence of the elastin that is contained in the basement membrane of the proximal airways and that participates to the axial backbone of the peripheral interstitial respiratory system. In smokers, a specific tobacco tar-induced fluorescence allows in vivo macrophage and alveolar wall imaging. Using 660 nm excitation and topical methylene blue, the technique enables cellular imaging of both bronchial epithelial layer and peripheral lung nodules. This article reviews the capabilities and possible limitations of confocal microendoscopy for in vivo proximal and distal lung explorations.

Published
2009
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3. Molecular predictive factors for progression of high-grade preinvasive bronchial lesions

Author

Suzanna Bota, Sophie Moreno-Swirc, Jeannette Bourguignon, Mathieu Salaün, Luc Thiberville, Richard Sesboüé, Josette Métayer, Laboratoire d'Informatique, de Traitement de l'Information et des Systèmes (LITIS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU), Génétique médicale et fonctionnelle du cancer et des maladies neuropsychiatriques, Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Service d'Anatomie et Cytologie Pathologique [CHU Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Service de pneumologie, oncologie thoracique et soins intensifs respiratoires [Rouen], Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-CHU Rouen, Supported by a research grant from the Comités Départementaux de la Ligue contre le Cancer (Eure et Seine Maritime), and the French Cancéropole Nord Ouest., Breton, Céline, Université Le Havre Normandie (ULH), Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA), Hôpital Charles Nicolle [Rouen], and Normandie Université (NU)-Normandie Université (NU)-CHU Rouen

Subjects
Male, Pathology, bronchoscopy, Biopsy, Loss of Heterozygosity, Critical Care and Intensive Care Medicine, [SDV.MHEP.PSR]Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, Loss of heterozygosity, MESH: Biopsy, 0302 clinical medicine, Bronchoscopy, Longitudinal Studies, MESH: Longitudinal Studies, Microdissection, MESH: Bronchial Neoplasms, MESH: Aged, MESH: Middle Aged, medicine.diagnostic_test, Bronchial Neoplasms, Middle Aged, follow-up studies, 3. Good health, MESH: Precancerous Conditions, 030220 oncology & carcinogenesis, MESH: Survival Analysis, Female, Microsatellite Instability, MESH: Disease Progression, Chromosomes, Human, Pair 3, Carcinoma in Situ, Pulmonary and Respiratory Medicine, Adult, medicine.medical_specialty, MESH: Chromosomes, Human, Pair 3, 03 medical and health sciences, disease progression, Intensive care, medicine, Humans, precancerous conditions, Aged, MESH: Loss of Heterozygosity, MESH: Carcinoma in Situ, MESH: Humans, business.industry, gene deletion, Carcinoma in situ, Microsatellite instability, Histology, MESH: Adult, medicine.disease, Survival Analysis, MESH: Male, 030228 respiratory system, MESH: Gene Deletion, [SDV.MHEP.PSR] Life Sciences [q-bio]/Human health and pathology/Pulmonology and respiratory tract, business, MESH: Female, MESH: Microsatellite Instability
Abstract

International audience; RATIONALE: The outcome of precancerous bronchial lesions is not well known, and their management is subject to controversy. Many molecular alterations are present in preinvasive lesions, but none has been assessed to predict the evolution of the lesions. OBJECTIVES: To analyze the outcome of high-grade precancerous lesions according to their molecular profile. METHODS: Twenty-three severe dysplasia and 31 carcinoma in situ (CIS) lesions in 37 patients were monitored using repeated autofluorescence bronchoscopy over a 12-year period. Microdissection and polymerase chain reaction analysis were performed on paraffin tissue sections to assess loss of heterozygosity (LOH) and microsatellite instability on chromosome 3p, 5q, and 9p. Histology and molecular status at baseline were compared between 7 lesions that became invasive, 11 that relapsed after treatment, 17 that were eradicated with local treatment, and 19 that spontaneously regressed. MEASUREMENTS AND MAIN RESULTS: Ninety-four percent of lesions that progressed or relapsed were CIS at baseline, whereas 79% of spontaneously regressing lesions were severe dysplasia (P < 0.0001). 3p and 9p LOH was more frequent in CIS than in severe dysplasia (P = 0.03). In the whole group of lesions as well as in the CIS group, 3p LOH was strongly associated with progression (P < 0.0001 and P = 0.02, respectively). Microsatellite instability was not associated with the outcome of the lesions. A therapeutic strategy based on the presence of 3p or 9p LOH would have led to overtreatment of six lesions but would have missed only 1 among the 18 progressing lesions. CONCLUSIONS: Baseline histology and 3p LOH analysis appear to be useful in predicting the outcome of high-grade precancerous lesions.

Published
2008
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4. Human in vivo confocal microscopic imaging of the distal bronchioles and alveoli

Author

Sophie Moreno-Swirc, Stéphane Dominique, Genevieve Bourg Heckly, Mathieu Salaün, Luc Thiberville, Breton, Céline, Service de pneumologie, oncologie thoracique et soins intensifs respiratoires [Rouen], Hôpital Charles Nicolle [Rouen], CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU), Laboratoire de Biophysique Moléculaire Cellulaire et Tissulaire (BIOMOCETI), Université Paris 13 (UP13)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Université de Rouen Normandie (UNIROUEN), and Normandie Université (NU)-Normandie Université (NU)-Hôpital Charles Nicolle [Rouen]-CHU Rouen

Subjects
Pulmonary and Respiratory Medicine, Pathology, medicine.medical_specialty, business.industry, Confocal, Critical Care and Intensive Care Medicine, 3. Good health, 03 medical and health sciences, 0302 clinical medicine, 030228 respiratory system, In vivo, Medical imaging, Microscopic imaging, Medicine, 030212 general & internal medicine, Cardiology and Cardiovascular Medicine, business, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2007

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