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Your search keyword '"Sandra Clauder‐Münster"' showing total 2 results
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2 results on '"Sandra Clauder‐Münster"'

1. Condensin controls cellular RNA levels through the accurate segregation of chromosomes instead of directly regulating transcription

Author

Esther Toselli, Claire Burny, Laurent Modolo, Xi-Ming Sun, Christian H. Haering, Samuel Marguerat, Pascal Bernard, Xavier Robellet, Lars M. Steinmetz, Clémence Hocquet, Sandra Clauder-Münster, Sara Cuylen-Haering, Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Statistique en grande dimension pour la génomique, Département PEGASE [LBBE] (PEGASE), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Dalian University of Technology, Department of Genetics [Stanford], Stanford Medicine, Stanford University-Stanford University, Laboratoire de biologie et modélisation de la cellule (LBMC UMR 5239), Université de Lyon-Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM), École normale supérieure de Lyon (ENS de Lyon)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), and École normale supérieure - Lyon (ENS Lyon)-Université Claude Bernard Lyon 1 (UCBL)

Subjects
Life Sciences & Biomedicine - Other Topics, 0301 basic medicine, SELECTIVE ELIMINATION, Cell division, Condensin, [SDV]Life Sciences [q-bio], S. cerevisiae, S Phase, Transcriptome, 0302 clinical medicine, Chromosome Segregation, Gene Expression Regulation, Fungal, Chromosome instability, Biology (General), ComputingMilieux_MISCELLANEOUS, GENE-EXPRESSION, Anaphase, Adenosine Triphosphatases, Regulation of gene expression, 0303 health sciences, Microscopy, Confocal, biology, condensin, General Neuroscience, 030302 biochemistry & molecular biology, Fungal genetics, General Medicine, Chromosomes and Gene Expression, READ ALIGNMENT, Cell biology, DNA-Binding Proteins, Medicine, COMPLEXES, Life Sciences & Biomedicine, COHESIN, Research Article, G2 Phase, chromosomes, QH301-705.5, Science, Green Fluorescent Proteins, FISSION YEAST, macromolecular substances, SMCs, POLYMERASE-II, Genomic Instability, General Biochemistry, Genetics and Molecular Biology, 03 medical and health sciences, Condensin complex, SCHIZOSACCHAROMYCES-POMBE, Schizosaccharomyces, MESSENGER-RNAS, Biology, Mitosis, 030304 developmental biology, Science & Technology, General Immunology and Microbiology, Cohesin, RNA-exosome, Gene Expression Profiling, RNA, Fungal, 030104 developmental biology, Multiprotein Complexes, Mutation, gene expression, biology.protein, 030217 neurology & neurosurgery, S. pombe
Abstract

Condensins are genome organisers that shape chromosomes and promote their accurate transmission. Several studies have also implicated condensins in gene expression, although the mechanisms have remained enigmatic. Here, we report on the role of condensin in gene expression in fission and budding yeasts. In contrast to previous studies, we provide compelling evidence that condensin plays no direct role in the maintenance of the transcriptome, neither during interphase nor during mitosis. We further show that the changes in gene expression in post-mitotic fission yeast cells that result from condensin inactivation are largely a consequence of chromosome missegregation during anaphase, which notably depletes the RNA-exosome from daughter cells. Crucially, preventing karyotype abnormalities in daughter cells restores a normal transcriptome despite condensin inactivation. Thus, chromosome instability, rather than a direct role of condensin in the transcription process, changes gene expression. This knowledge challenges the concept of gene regulation by canonical condensin complexes.

Published
2018
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2. Expression of Nuclear and Mitochondrial Genes Encoding ATP Synthase Is Synchronized by Disassembly of a Multisynthetase Complex

Author

Mathieu Frechin, Philippe Hammann, Emmanuel Tetaud, Gaétan Bader, Ludovic Enkler, Lars M. Steinmetz, Robert P. Martin, Bruno Senger, Sandra Clauder-Münster, Corinne Blancard, Hubert Dominique Becker, Jean-Paul di Rago, Daphné Laporte, Architecture et réactivité de l'ARN (ARN), Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS), Génétique moléculaire, génomique, microbiologie (GMGM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Institut de biologie moléculaire et cellulaire (IBMC), and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)

Subjects
Mitochondrial DNA, Saccharomyces cerevisiae Proteins, Mitochondrial translation, [SDV]Life Sciences [q-bio], Gene Expression, Oxidative phosphorylation, Saccharomyces cerevisiae, Mitochondrion, 03 medical and health sciences, 0302 clinical medicine, Multienzyme Complexes, Gene Expression Regulation, Fungal, Gene expression, Molecular Biology, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, Regulation of gene expression, Cell Nucleus, 0303 health sciences, ATP synthase, biology, RNA-Binding Proteins, Cell Biology, Molecular biology, Cell biology, Mitochondria, Cytosol, Proton-Translocating ATPases, biology.protein, Protein Multimerization, 030217 neurology & neurosurgery
Abstract

In eukaryotic cells, oxidative phosphorylation involves multisubunit complexes of mixed genetic origin. Assembling these complexes requires an organelle-independent synchronizing system for the proper expression of nuclear and mitochondrial genes. Here we show that proper expression of the F1FO ATP synthase (complex V) depends on a cytosolic complex (AME) made of two aminoacyl-tRNA synthetases (cERS and cMRS) attached to an anchor protein, Arc1p. When yeast cells adapt to respiration the Snf1/4 glucose-sensing pathway inhibits ARC1 expression triggering simultaneous release of cERS and cMRS. Free cMRS and cERS relocate to the nucleus and mitochondria, respectively, to synchronize nuclear transcription and mitochondrial translation of ATP synthase genes. Strains releasing asynchronously the two aminoacyl-tRNA synthetases display aberrant expression of nuclear and mitochondrial genes encoding subunits of complex V resulting in severe defects of the oxidative phosphorylation mechanism. This work shows that the AME complex coordinates expression of enzymes that require intergenomic control.

Published
2014
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