1. Utilization of Grape Seed Flour for Antimicrobial Lipopeptide Production by Bacillus amyloliquefaciens C5 Strain
- Author
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Siwar Soussi, Thierry Jouenne, Ferid Limam, Julie Hardouin, Dorra Gharbi, Olfa Tabbene, Pascal Cosette, Rym Essid, Salem Elkahoui, Laboratoire des Substances Bioactives (Centre de Biotechnologie de Borj Cedria, Hammam-Lif) (LSBA), Centre de Biotechnologie de Borj Cédria (Hammam-Lif, Tunisie), Polymères Biopolymères Surfaces (PBS), Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut de Chimie du CNRS (INC)-Institut Normand de Chimie Moléculaire Médicinale et Macromoléculaire (INC3M), Institut de Chimie du CNRS (INC)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut national des sciences appliquées Rouen Normandie (INSA Rouen Normandie), Institut National des Sciences Appliquées (INSA)-Normandie Université (NU)-Institut National des Sciences Appliquées (INSA)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)-Université de Caen Normandie (UNICAEN), Normandie Université (NU)-École Nationale Supérieure d'Ingénieurs de Caen (ENSICAEN), Normandie Université (NU)-Université Le Havre Normandie (ULH), and Normandie Université (NU)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
0106 biological sciences ,Grape seed flour ,Bacillus amyloliquefaciens ,M9 mineral medium ,[SDV]Life Sciences [q-bio] ,Flour ,Bioengineering ,Microbial Sensitivity Tests ,Antimicrobial activity ,01 natural sciences ,Applied Microbiology and Biotechnology ,Biochemistry ,Lipopeptide ,Lipopeptides ,Bacillomycin ,chemistry.chemical_compound ,Anti-Infective Agents ,010608 biotechnology ,Vitis ,[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology ,Food science ,Molecular Biology ,Strain (chemistry) ,biology ,010405 organic chemistry ,General Medicine ,biology.organism_classification ,Antimicrobial ,Thin-layer chromatography ,0104 chemical sciences ,MALDI-TOF/MS ,chemistry ,Seeds ,Antibacterial activity ,Surfactin ,Biotechnology - Abstract
International audience; An endophytic Bacillus amyloliquefaciens strain called C5, able to produce biosurfactant lipopeptides with a broad antibacterial activity spectrum, has been isolated from the roots of olive tree. Optimization of antibacterial activity was undertaken using grape seed flour (GSF) substrate at 0.02, 0.2, and 2% (w/v) in M9 medium. Strain C5 exhibited optimal growth and antimicrobial activity (MIC value of 60 μg/ml) when incubated in the presence of 0.2% GSF while lipopeptide production culminated at 2% GSF. Thin layer chromatography analysis of lipopeptide extract revealed the presence of at least three active spots at Rf 0.35, 0.59, and 0.72 at 0.2% GSF. Data were similar to those obtained in LB-rich medium. MALDI-TOF/MS analysis of lipopeptide extract obtained from 0.2% GSF substrate revealed the presence of surfactin and bacillomycin D. These results show that GSF could be used as a low-cost culture medium supplement for optimizing the production of biosurfactants by strain C5.
- Published
- 2019
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