1. Improved response of triploid citrus varieties to water deficit is related to anatomical and cytological properties
- Author
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Yann Froelicher, Stéphane Herbette, Raphaël Morillon, Radia Lourkisti, Liliane Berti, Yann Quilichini, Julie Oustric, Jérémie Santini, Laboratoire de Biochimie et Biologie Moléculaire Végétales - UMR6134, Université Pascal Paoli (UPP)-Centre National de la Recherche Scientifique (CNRS), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Laboratoire de Physique et Physiologie Intégratives de l’Arbre en environnement Fluctuant (PIAF), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Clermont Auvergne (UCA), 'Collectivite de Corse' as part of the research project 'Innov'agrumes' (FEDER), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
- Subjects
0106 biological sciences ,0301 basic medicine ,Citrus ,Physiology ,F60 - Physiologie et biochimie végétale ,[SDV]Life Sciences [q-bio] ,Plant Science ,Biology ,Photosynthesis ,01 natural sciences ,Polyploidy ,03 medical and health sciences ,Leaf gas exchange ,Polyploid ,Genetics ,Water deficit ,2. Zero hunger ,Tangor ,Stomatal response ,Water ,food and beverages ,Hydrogen Peroxide ,15. Life on land ,biology.organism_classification ,Triploidy ,Photosynthetic capacity ,Chloroplast ultrastructure ,Citrange ,Plant Breeding ,Horticulture ,Résistance à la sécheresse ,030104 developmental biology ,Oxidative status ,Ploidy ,Rootstock ,Clementine ,010606 plant biology & botany - Abstract
International audience; Polyploidy plays a major role in citrus plant breeding to improve the adaptation of polyploid rootstocks as well as scions to adverse conditions and to enhance agronomic characteristics. In Citrus breeding programs, triploidy could be a useful tool to react to environmental issues and consumer demands because the produced fruits are seedless. In this study, we compared the physiological, biochemical, morphological, and ultrastructural responses to water deficit of triploid and diploid citrus varieties obtained from 'Fortune' mandarin and `Ellendale' tangor hybridization. One diploid clementine tree was included and used as a reference. All studied scions were grafted on C-35 citrange rootstock. Triploidy decreased stomatal density and increased stomata size. The number of chloroplasts increased in 3x varieties. These cytological properties may explain the greater photosynthetic capacity (Pnet, gs, Fv/Fm) and enhanced water-holding capacity (RWC, proline). In addition, reduced degradation of ultrastructural organelles (chloroplasts and mitochondria) and thylakoids accompanied by less photosynthetic activity and low oxidative damages were found in 3x varieties. Triploid varieties, especially T40-3x, had a better ability to limit water loss and dissipate excess energy (NPQ) to protect photosystems. Higher starch reserves in 3x varieties suggest a better carbon and energy supply and increases in plastoglobuli size suggest less oxidative damage (H2O2, MDA), especially in T40-3x, and preservation of photosynthetic apparatus. Taken together, our results suggest that desirable cytological and ultrastructural traits induced by triploidy improve water stress response and could be a useful stress marker during environmental constraints.
- Published
- 2021
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