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5 results on '"Oya Tagit"'

1. Colloidal Nanoparticles for Signal Enhancement in Optical Diagnostic Assays

Author

Niko Hildebrandt, Oya Tagit, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11), NanoBioPhotonics (NANO), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], [SDV]Life Sciences [q-bio], Biomedical Engineering, Nanoparticle, Bioengineering, Nanotechnology, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Quantum Dots, Animals, Humans, General Materials Science, Colloids, Surface plasmon resonance, Metal nanoparticles, General Chemistry, Surface Plasmon Resonance, 021001 nanoscience & nanotechnology, Condensed Matter Physics, 0104 chemical sciences, Highly sensitive, Signal enhancement, Colloidal nanoparticles, Nanomedicine, Spectrometry, Fluorescence, Quantum dot, Nanoparticles, 0210 nano-technology, Biomarkers
Abstract

Item does not contain fulltext The use of nanotechnologies for the development of highly sensitive and affordable diagnostic assays has significantly improved the ability to detect and characterize multiple types of biomarkers. Semiconductor and metal nanoparticles with unique optical properties have been successfully integrated within biomarker detection schemes for the generation and enhancement of optical signals in label-based and label-free assays. Highly sensitive label-based diagnostics has been realized particularly via using quantum dots (QDs) as labeling probes. Similarly, many label-free techniques that are emerging as potential complements to label-based approaches benefit from signal enhancement strategies using e.g., metal nanoparticles. This review presents a concise overview of recent advances in diagnostic assays that utilize nanoparticles for the generation and enhancement of optical signals in fluorescence- and surface plasmon resonance-based techniques. Advanced diagnostic assays that utilize nanoparticles provide major improvements in detection sensitivity, which can potentially meet the challenging requirements of clinical diagnostics.

Published
2018
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2. Sensitivity Enhancement of Förster Resonance Energy Transfer Immunoassays by Multiple Antibody Conjugation on Quantum Dots

Author

Oya Tagit, Giacomo Annio, Travis L. Jennings, Niko Hildebrandt, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11), NanoBioPhotonics (NANO), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and ANR-16-CE09-0015,NEUTRINOS,Suivi des interactions biologiques par détection optique ultrasensible à base de nanoparticules(2016)

Subjects
Immunoconjugates, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], [SDV]Life Sciences [q-bio], Biomedical Engineering, Pharmaceutical Science, Nanoparticle, Bioengineering, 02 engineering and technology, Biosensing Techniques, 010402 general chemistry, 01 natural sciences, All institutes and research themes of the Radboud University Medical Center, Quantum Dots, medicine, Fluorescence Resonance Energy Transfer, Humans, Terbium, Pharmacology, chemistry.chemical_classification, Immunoassay, Luminescent Agents, medicine.diagnostic_test, biology, Chemistry, Biomolecule, Organic Chemistry, Prostate-Specific Antigen, 021001 nanoscience & nanotechnology, 0104 chemical sciences, Förster resonance energy transfer, Quantum dot, Homogeneous, Immunoglobulin G, biology.protein, Biophysics, Antibody, 0210 nano-technology, Biotechnology, Conjugate
Abstract

International audience; Quantum dots (QDs) are not only advantageous for color-tuning, improved brightness, and high stability, but their nanoparticle surfaces also allow for the attachment of many biomolecules. Because IgG antibodies (AB) are in the same size range of biocompatible QDs and the AB orientation after conjugation to the QD is often random, it is difficult to predict if few or many AB per QD will lead to an efficient AB-QD conjugate. This is particularly true for homogeneous Förster resonance energy transfer (FRET) sandwich immunoassays, for which the AB on the QD must bind a biomarker that needs to bind a second AB-FRET-conjugate. Here, we investigate the performance of Tb-to-QD FRET immunoassays against total prostate specific antigen (TPSA) by changing the number of AB per QD while leaving all the other assay components unchanged. We first characterize the AB-QD conjugation by various spectroscopic, microscopic, and chromatographic techniques and then quantify the TPSA immunoassay performance regarding sensitivity, limit of detection, and dynamic range. Our results show that an increasing conjugation ratio leads to significantly enhanced FRET immunoassays. These findings will be highly important for developing QD-based immunoassays in which the concentrations of both AB and QDs can significantly influence the assay performance.

Published
2018
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3. Fluorescence Sensing of Circulating Diagnostic Biomarkers Using Molecular Probes and Nanoparticles

Author

Oya Tagit, Niko Hildebrandt, Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), NanoBioPhotonics (NANO), Département Biochimie, Biophysique et Biologie Structurale (B3S), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

Subjects
Materials science, Cancer development and immune defence Radboud Institute for Molecular Life Sciences [Radboudumc 2], [SDV]Life Sciences [q-bio], Nanoparticle, Bioengineering, Fluorescence sensing, Nanotechnology, quantum dots, 02 engineering and technology, 010402 general chemistry, 01 natural sciences, Fluorescence, Diagnostic biomarker, Disease biomarker, Instrumentation, clinical diagnostics, Fluid Flow and Transfer Processes, business.industry, Process Chemistry and Technology, 021001 nanoscience & nanotechnology, 3. Good health, 0104 chemical sciences, immunoassays, Förster resonance energy transfer, FRET, Nanoparticles, biosensing, Photonics, 0210 nano-technology, Molecular probe, business, Biosensor
Abstract

The interplay of photonics, nanotechnology, and biochemistry has significantly improved the identification and characterization of multiple types of biomarkers by optical biosensors. Great achievements in fluorescence-based technologies have been realized, for example, by the advancement of multiplexing techniques or the introduction of nanoparticles to biochemical and clinical research. This review presents a concise overview of recent advances in fluorescence sensing techniques for the detection of circulating disease biomarkers. Detection principles of representative approaches, including fluorescence detection using molecular fluorophores, quantum dots, and metallic and silica nanoparticles, are explained and illustrated by pertinent examples from the recent literature. Advanced detection technologies and material development play a major role in modern biosensing and consistently provide significant improvements toward robust, sensitive, and versatile platforms for early detection of circulating diagnostic biomarkers.

Published
2017
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4. Terbium to quantum rod Förster resonance energy transfer for homogeneous bioassays with picomolar detection limits

Author

Oya Tagit, Giacomo Annio, Niko Hildebrandt, Radboud Institute for Molecular Life Sciences [Nijmegen, the Netherlands], Institut de Biologie Intégrative de la Cellule (I2BC), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)

Subjects
[PHYS]Physics [physics], Photoluminescence, Chemistry, [SDV]Life Sciences [q-bio], technology, industry, and agriculture, Analytical chemistry, chemistry.chemical_element, Terbium, Acceptor, Analytical Chemistry, Förster resonance energy transfer, [CHIM]Chemical Sciences, Nanorod, Luminescence, Spectroscopy, Biosensor, ComputingMilieux_MISCELLANEOUS
Abstract

We report on the proof-of-principle of using semiconductor nanorods (quantum rods; QRs) in homogeneous Forster resonance energy transfer (FRET) bioassays. Terbium complexes (Tb) with long photoluminescence lifetimes were used as FRET donors, and biological recognition was accomplished by biotin-QR to Tb-streptavidin binding. Time-resolved and steady-state spectroscopy were used to investigate varying relative donor/acceptor concentrations and different lengths of polyethylene glycol (PEG)-based surface coatings. Homogeneous bioassays displayed low picomolar detection limits in 150 μL samples, independent of whether 1 and 10 kDa biotin-PEG-SH surface ligands were used. The results suggest that the combination of Tb-to-QR FRET with time-gated detection may become a powerful tool for homogeneous biosensing.

Published
2015
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5. Transport Mechanisms of Squalenoyl-Adenosine Nanoparticles Across the Blood–Brain Barrier

Author

Niko Hildebrandt, Oya Tagit, Didier Desmaële, Patrick Couvreur, Valérie Nicolas, Dunja Sobot, Kevin Braeckmans, Sinda Lepetre-Mouelhi, Julie Mougin, Stefaan C. De Smedt, Alice Gaudin, Thomas Martens, Karine Andrieux, Institut Galien Paris-Sud (IGPS), Université Paris-Sud - Paris 11 (UP11)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Radboud Institute for Molecular Life Sciences [Nijmegen, the Netherlands], Laboratoire d'Innovation pour les Technologies des Energies Nouvelles et les nanomatériaux (LITEN), Institut National de L'Energie Solaire (INES), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Centre National de la Recherche Scientifique (CNRS), Universiteit Gent = Ghent University (UGENT), Biophotonic Imaging Group, Institut Paris Saclay d’Innovation Thérapeutique (IPSIT), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Physico-chimie, pharmacotechnie, biopharmacie (PCPB), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Unité de Technologies Chimiques et Biologiques pour la Santé (UTCBS - UM 4 (UMR 8258 / U1022)), Ecole Nationale Supérieure de Chimie de Paris - Chimie ParisTech-PSL (ENSCP), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Universiteit Gent = Ghent University [Belgium] (UGENT), Institut Paris-Saclay d'Innovation Thérapeutique (IPSIT), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris-Sud - Paris 11 (UP11), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC), Institut Galien Paris-Saclay (IGPS), Institut de Chimie du CNRS (INC)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Ghent University [Belgium] (UGENT), Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Université Paris-Sud - Paris 11 (UP11)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), and Ecole Nationale Supérieure de Chimie de Paris- Chimie ParisTech-PSL (ENSCP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)

Subjects
General Chemical Engineering, [SDV]Life Sciences [q-bio], Drug delivery to the brain, Nanoparticle, 02 engineering and technology, Pharmacology, Blood–brain barrier, 03 medical and health sciences, Materials Chemistry, medicine, [CHIM]Chemical Sciences, ComputingMilieux_MISCELLANEOUS, 030304 developmental biology, [PHYS]Physics [physics], 0303 health sciences, Chemistry, General Chemistry, Receptor-mediated endocytosis, 021001 nanoscience & nanotechnology, Adenosine, 3. Good health, medicine.anatomical_structure, Transcytosis, Biochemistry, Drug delivery, Nanomedicine, 0210 nano-technology, medicine.drug
Abstract

Drug delivery to the brain is one of the major challenges in the treatment of cerebral diseases and implies extensive understanding of nanomedicine transcytosis pathways across the blood–brain barr...

Published
2015
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