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1. Myostatin and the skeletal muscle atrophy and hypertrophy signaling pathways

Author

Jean-Charles Gabillard, Iban Seiliez, Isabelle Cassar-Malek, Julie Rodriguez, Ilham Chelh, Barbara Vernus, Anne Bonnieu, Brigitte Picard, A. Hadj Sassi, Dynamique Musculaire et Métabolisme (DMEM), Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM), Université de Montpellier (UM), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), USC2009 - 1, Avenue des Facultés, Institut National de la Recherche Agronomique (INRA), Université Sciences et Technologies - Bordeaux 1, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), ANR-08-BLAN-0267,MYOTROPHY,Le rôle de myostatine dans les voies de signalisation régulant la balance atrophie/hypertrophie dans le muscle squelettique(2008), Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), ANR Myotrophy, Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Dynamique Musculaire et Métabolisme ( DMEM ), Institut National de la Recherche Agronomique ( INRA ) -Université de Montpellier ( UM ), Université de Montpellier ( UM ), Différenciation Cellulaire et Croissance ( DCC ), Institut National de la Recherche Agronomique ( INRA ) -Université Montpellier 2 - Sciences et Techniques ( UM2 ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Laboratoire de Physiologie et Génomique des Poissons ( LPGP ), Institut National de la Recherche Agronomique ( INRA ) -Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Institut National de la Recherche Agronomique ( INRA ), Nutrition, Aquaculture et Génomique ( NUAGE ), and Institut National de la Recherche Agronomique ( INRA ) -Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER )

Subjects
medicine.medical_specialty, muscle differentiation, Myostatin, Protein degradation, [ SDV.BA ] Life Sciences [q-bio]/Animal biology, growth differentiation factor-8, Muscle hypertrophy, 03 medical and health sciences, Cellular and Molecular Neuroscience, 0302 clinical medicine, Internal medicine, Myokine, medicine, Humans, [ SDV.BDD ] Life Sciences [q-bio]/Development Biology, Muscle, Skeletal, Molecular Biology, [SDV.BDD]Life Sciences [q-bio]/Development Biology, PI3K/AKT/mTOR pathway, Cell Proliferation, 030304 developmental biology, Pharmacology, 0303 health sciences, biology, TOR Serine-Threonine Kinases, [SDV.BA]Life Sciences [q-bio]/Animal biology, Skeletal muscle, Cell Differentiation, muscle homeostasis, Hypertrophy, Cell Biology, musculoskeletal system, Muscle atrophy, Muscular Atrophy, Endocrinology, medicine.anatomical_structure, GDF11, biology.protein, protein degradation, translational machinery, mTOR, Molecular Medicine, medicine.symptom, 030217 neurology & neurosurgery, Signal Transduction
Abstract

Myostatin, a member of the transforming growth factor-β superfamily, is a potent negative regulator of skeletal muscle growth and is conserved in many species, from rodents to humans. Myostatin inactivation can induce skeletal muscle hypertrophy, while its overexpression or systemic administration causes muscle atrophy. As it represents a potential target for stimulating muscle growth and/or preventing muscle wasting, myostatin regulation and functions in the control of muscle mass have been extensively studied. A wealth of data strongly suggests that alterations in skeletal muscle mass are associated with dysregulation in myostatin expression. Moreover, myostatin plays a central role in integrating/mediating anabolic and catabolic responses. Myostatin negatively regulates the activity of the Akt pathway, which promotes protein synthesis, and increases the activity of the ubiquitin-proteasome system to induce atrophy. Several new studies have brought new information on how myostatin may affect both ribosomal biogenesis and translation efficiency of specific mRNA subclasses. In addition, although myostatin has been identified as a modulator of the major catabolic pathways, including the ubiquitin-proteasome and the autophagy-lysosome systems, the underlying mechanisms are only partially understood. The goal of this review is to highlight outstanding questions about myostatin-mediated regulation of the anabolic and catabolic signaling pathways in skeletal muscle. Particular emphasis has been placed on (1) the cross-regulation between myostatin, the growth-promoting pathways and the proteolytic systems; (2) how myostatin inhibition leads to muscle hypertrophy; and (3) the regulation of translation by myostatin.

Published
2014

2. Le phénotypage de l’efficacité alimentaire et de ses composantes, une nécessité pour accroître l’efficience des productions animales

Author

Mathilde Dupont-Nivet, J. Geurden, Françoise Médale, J. Y. Dourmad, Sandrine Mignon-Grasteau, Hélène Gilbert, Jacques Agabriel, Florence Phocas, Génétique Animale et Biologie Intégrative (GABI), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Unité de Recherches Avicoles (URA), Institut National de la Recherche Agronomique (INRA), Génétique Physiologie et Systèmes d'Elevage (GenPhySE ), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] (PEGASE), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Florence Phocas, Génétique Animale et Biologie Intégrative ( GABI ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), Nutrition, Aquaculture et Génomique ( NUAGE ), Institut National de la Recherche Agronomique ( INRA ) -Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ), Recherches Avicoles ( SRA ), Institut National de la Recherche Agronomique ( INRA ), GenPhySE - UMR 1388 ( Génétique Physiologie et Systèmes d'Elevage ), Institut National de la Recherche Agronomique ( INRA ) -École nationale supérieure agronomique de Toulouse [ENSAT]-ENVT, Physiologie, Environnement et Génétique pour l'Animal et les Systèmes d'Elevage [Rennes] ( PEGASE ), Institut National de la Recherche Agronomique ( INRA ) -AGROCAMPUS OUEST, Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Recherches Avicoles (SRA), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Vétérinaire de Toulouse (ENVT), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-École nationale supérieure agronomique de Toulouse [ENSAT], and AGROCAMPUS OUEST-Institut National de la Recherche Agronomique (INRA)

Subjects
2. Zero hunger, ration alimentaire, sélection génétique, [ SDV ] Life Sciences [q-bio], alimentation animale, [SDV]Life Sciences [q-bio], rejet d'élevage, valeur énergétique, efficacité protéique, impact environnemental, efficacité énergétique, efficacité métabolique, efficacité digestive
Abstract

Dans un contexte de demande croissante en protéines animales, l’élevage de précision et la sélection animale doivent viser à accroître la compétitivité des systèmes de production et à réduire leur impact environnemental. Les animaux doivent être plus efficaces pour transformer leur ration (réduire l’ingéré tout en maintenant une production stable), en acceptant des ressources alimentaires variées (protéines ou fibres) et en minimisant leurs rejets (totaux ou leurs composantes N et P, GES). Toutefois, l’efficacité alimentaire est un phénotype complexe qui doit être phénotypé finement pour comprendre les processus biologiques sous-jacents avant d’identifier les leviers d’action pour l’améliorer (efficacité digestive vs métabolique ; efficacité énergétique vs protéique). Ce phénotypage doit être réalisé en conditions variées tant sur le plan du génotype, du niveau alimentaire que de la composition de la ration (teneur en fibres, en acides aminés). Il s’agit aussi d’envisager une approche prédictive de l’ingéré et de l’efficacité alimentaire basée sur des paramètres peu coûteux et faciles à mesurer (croissance, bio-marqueurs…) dans le cadre d’un élevage de précision ou de programmes de sélection génétique, car le phénotypage à large échelle de l’ingéré reste coûteux et parfois difficile à mettre en oeuvre. Pour évaluer l’efficience globale des systèmes de productions animales, il s’agit de considérer les réponses des animaux pour des génotypes et des régimes alimentaires contrastés en termes d’efficacité alimentaire et de rejets en quantité et en qualité (GES, N, P), mais aussi de robustesse des animaux (comportement alimentaire, activité générale, santé)., In a context of an increasing demand for animal proteins, precision farming and animal breeding must be goal-oriented to improve production competitiveness and to reduce environmental impact. Animals have to be more efficient in transforming their diet (reducing their feed intake while maintaining production performance), accepting various feeds (protein and fibers) and reducing their wastes (total, N, P, GHG…). However, feed efficiency is a complex phenotype that needs to be deeply phenotyped. This will allow unravelling the biological processes to identify ways to improve feed efficiency (digestive versus metabolic efficiency; energetic versus protein efficiency) according to the genotype, the feed level and the diet composition (fibre content, AA content). Predictive biology of feed intake and feed efficiency is also needed using cost-effective and easy to record traits (growth, biomarkers…) for precision farming and for selective breeding, because large-scale phenotyping for feed intake remains costly. To assess the efficiency of livestock production systems, there is a need to reconsider animal responses according to contrasted genotypes and feeds not only for feed efficiency and wastes in quantity and quality (GHG, N, P), but also for robustness of animals (feeding behavior, general activity, health).

Published
2014

3. Dietary vitamin D3 level affects the expression of myogenic regulatory factors and myosin heavy chain in European sea bass (Dicentrarchus labrax) larvae

Author

Alami-Durante, Hélène, Cluzeaud, Marianne, Mazurais, David, Zambonino-Infante, José, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Unité mixte de recherche nutrition aquaculture et génomique (NUAGE), Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SDV]Life Sciences [q-bio], [INFO]Computer Science [cs], [INFO] Computer Science [cs], ComputingMilieux_MISCELLANEOUS, BAR COMMUN
Abstract

International audience

Published
2010

4. Regulation of FADS2 expression and activity in European sea bass (Dicentrarchus labrax, L.) fed a vegetable diet

Author

E. Santigosa I Culi, José-Luis Zambonino-Infante, F. Geay, Laurent Corcos, Pierre Boudry, Chantal Cahu, Charlotte Corporeau, Marc Vandeputte, Y. Dreano, Nathalie Bodin, David Mazurais, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Physiologie et Ecophysiologie des Mollusques Marins (PE2M), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Centre National de la Recherche Scientifique (CNRS), INSERM, U613, Université de Brest (UBO), Faculté de Médecine, Université européenne de Bretagne - European University of Brittany (UEB), U613, UMR 212 EME 'écosystèmes marins exploités' (EME), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Montpellier (UM), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), UMR 1067 : Nutrition, Aquaculture et Génomique, Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique ( INRA ), Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ), Physiologie et Ecophysiologie des Mollusques Marins ( PE2M ), Université de Caen Normandie ( UNICAEN ), Normandie Université ( NU ) -Normandie Université ( NU ) -Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ) -Centre National de la Recherche Scientifique ( CNRS ), Université de Brest ( UBO ), Université européenne de Bretagne ( UEB ), UMR 212 EME 'écosystèmes marins exploités' ( EME ), Université de Montpellier ( UM ) -Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ) -Institut de Recherche pour le Développement ( IRD ), Génétique Animale et Biologie Intégrative ( GABI ), Institut National de la Recherche Agronomique ( INRA ) -AgroParisTech, Nutrition, Aquaculture et Génomique ( NUAGE ), Institut National de la Recherche Agronomique ( INRA ) -Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer ( IFREMER ), AgroParisTech-Institut National de la Recherche Agronomique (INRA), and Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)

Subjects
Fatty Acid Desaturases, Physiology, [SDV]Life Sciences [q-bio], Aquaculture, Biochemistry, Linseed oil, Vegetables, Food science, 0303 health sciences, Fatty Acids, Sea bass (Dicentrarchus labrax), food and beverages, 04 agricultural and veterinary sciences, Fish oil, Eicosapentaenoic acid, Intestines, Liver, Docosahexaenoic acid, Sterol binding, Sterol Regulatory Element Binding Protein 1, sea bass, Fish Proteins, food.ingredient, HUFA biosynthesis, Biology, 03 medical and health sciences, food, Fish meal, Animals, PPAR alpha, 14. Life underwater, Sea bass, Molecular Biology, 030304 developmental biology, FADS2 expression and activity, [ SDV ] Life Sciences [q-bio], business.industry, HUFA, Animal Feed, Diet, Biotechnology, Gene Expression Regulation, hufa biosynthesis, fads2 expression and activity, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Bass, biosynthesis, business
Abstract

Chantier qualité GA; Supplies of marine fish oils are limited, and continued growth in aquaculture production dictates that lipid substitutes in fish diets must be used without compromising fish health and product quality. In this study, the total substitution of a fish meal and fish oil by a blend of vegetable meals (corn, soybean, wheat and lupin) and linseed oil in the diet of European sea bass (Dicentrachus labrax) was investigated. Two groups of European sea bass were fed with fish diet (FD) or vegetable diet (VD) for 9 months. VD, totally deprived of eicosapentaenoate (EPA; 20:5n-3) and docosahexaenoate (DHA; 22:6n-3), revealed a nutritional deficiency and affected growth performance. Whilst VD induced a significant increase in fatty acid desaturase 2 (FADS2) and sterol binding regulatory element-binding protein 1 (SREBP-1) mRNA levels, the desaturation rate of [1-(14)C]18:3n-3 into [1-(14)C]18:4n-3, analysed in microsomal preparations using HPLC method, did not show an upregulation of FADS2 activities in liver and intestine of fish fed VD. Moreover Western-blot analysis did not revealed any significant difference of FADS2 protein amount between the two dietary groups. These data demonstrate that sea bass exhibits a desaturase (FADS2) activity whatever their diet, but a post-transcriptional regulation of fads2 RNA prevents an increase of enzyme in fish fed a HUFA-free diet. This led to a lower fish growth and poor muscle HUFA content. (C) 2010 Elsevier Inc. All rights reserved.

Published
2010

5. Metabolomics and fish nutrition: a review in the context of sustainable feed development

Author

Sandrine Skiba-Cassy, Nadège Richard, Annick Moing, Benoit Fauconneau, Catherine Deborde, Simon Roques, Biologie du fruit et pathologie (BFP), Université de Bordeaux (UB)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Nutrition, Métabolisme, Aquaculture (NuMéA), Université de Pau et des Pays de l'Adour (UPPA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Phileo Lesaffre Animal Care, Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
sampling, Fish farming, Context (language use), Management, Monitoring, Policy and Law, Aquatic Science, Partial substitution, Biology, 03 medical and health sciences, Metabolomics, Aquaculture, 14. Life underwater, 030304 developmental biology, 2. Zero hunger, 0303 health sciences, Ecology, business.industry, fish nutrition, Fish fillet, 04 agricultural and veterinary sciences, metabolomics, Biotechnology, aquaculture, 13. Climate action, sustainable feed, 040102 fisheries, 0401 agriculture, forestry, and fisheries, %22">Fish , business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Omics technologies
Abstract

International audience; Aquaculture is facing a strategic challenge to improve feed suitability and support the global increase in fish production. Improvements in diet formulation for sustainable nutritional strategies have focused to date on the partial substitution of marine resources by plant resources but will now include other alternative feed-stuffs. Growth trials and body composition data provide valuable indicators of fish nutritional status, while omics technologies may contribute to a better understanding of fish nutrition and help to demonstrate how feed and nutrients act in fish metabolism. Metabolomic approaches give an insight into fish metabolism through a non-targeted analysis of metabolites in tissues or biofluids that involve multiple factors affecting fish, such as nutrition. In this review, we highlight the outcomes of publications in metabolomics applied to fish nutrition. We explain the concept of metabolomics and discuss specific technical considerations related to sample type, sampling and sample preparation. We show how metabolomic studies help to elucidate the impact of nutrition on fish fillet composition and fish metabolism. Finally, we describe the potential applications of metabolomic approaches for the non-invasive monitoring of fish nutritional status.

Published
2020

6. Fish facing global change: are early stages the lifeline?

Author

David Mazurais, Marie Vagner, José-Luis Zambonino-Infante, LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), LIttoral ENvironnement et Sociétés (LIENSs), La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés - UMR 7266 (LIENSs), Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0106 biological sciences, Aquatic Organisms, Developmental plasticity, Acclimatization, [SDE.MCG]Environmental Sciences/Global Changes, [SDV]Life Sciences [q-bio], Population, Aquatic Science, Biology, Oceanography, Affect (psychology), 010603 evolutionary biology, 01 natural sciences, Acidification, Animals, Ecosystem, 14. Life underwater, education, Hypoxia, Organism, ComputingMilieux_MISCELLANEOUS, Phenotypic plasticity, education.field_of_study, Ecology, Marine fishes, 010604 marine biology & hydrobiology, ACL, Fishes, Temperature, Global change, General Medicine, Pollution, Early environmental history, [SDE]Environmental Sciences, %22">Fish , [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

International audience; The role of phenotypic plasticity in the acclimation and adaptive potential of an organism to global change is not currently accounted for in prediction models. The high plasticity of marine fishes is mainly attributed to their early stages, during which morphological, structural and behavioural 'functions are particularly sensitive to environmental constraints. This developmental plasticity can determine later physiological performances and fitness, and may further affect population dynamics and ecosystem functioning. This review asks the essential question of what role early stages play in the ability of fish to later cope with the effects of global change, considering three key environmental factors (temperature, hypoxia and acidification). After having identified the carry-over effects of early exposure reported in the literature, we propose areas that we believe warrant the most urgent attention for further research to better understand the role of developmental plasticity in the responses of marine organisms to global change.

Published
2019

7. Effect of dietary selenium in rainbow trout (Oncorhynchus mykiss) broodstock on antioxidant status, its parental transfer and oxidative status in the progeny

Author

Brice Bouyssiere, Sandra Mounicou, Stéphanie Fontagné-Dicharry, Maroussia Parailloux, P. Antony Jesu Prabhu, Mickael Briens, Benoit Fauconneau, Pauline Wischhusen, Sadasivam Kaushik, Maïté Bueno, Pierre-André Geraert, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Adisseo France SAS, Institut des sciences analytiques et de physico-chimie pour l'environnement et les materiaux (IPREM), Université de Pau et des Pays de l'Adour (UPPA)-Centre National de la Recherche Scientifique (CNRS), Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Université de Pau et des Pays de l'Adour (UPPA)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Institute of Marine Research [Bergen] (IMR), University of Bergen (UiB), and The authors have no conflict of interest to declare for this work that was labelled by the 'Institut Carnot France Futur Elevage (IC-F2E) contract number 22001062'. This present study was supported by I-site E2S: Energy and Environment Solutions from the University of Pau and Pays de l'Adour, UPPA contract 2017-17 (P.W., PhD fellowship) and Institute of Marine Research (IMR) (P.A.J.P., ParSel Project no. 15329).

Subjects
Milt, Antioxidant, Broodstock, medicine.medical_treatment, Aquatic Science, Biology, 03 medical and health sciences, Selenium, Animal science, Progeny, medicine, 14. Life underwater, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Vitamin C, Vitamin E, Glutathione peroxidase, 04 agricultural and veterinary sciences, Ascorbic acid, Rainbow trout, chemistry, 040102 fisheries, 0401 agriculture, forestry, and fisheries, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

International audience; We studied the effects of supplementing a plant-ingredient based rainbow trout broodstock diet with different forms of selenium (Se) on reproductive performance, parental Se transfer and antioxidant metabolism in their progeny before first-feeding. Three groups of rainbow trout were fed a diet either without selenium supplementation (NC, basal Se level: 0.3 ppm) or supplemented with 0.3 ppm Se either in the form of sodium selenite (SS) or as hydroxy-selenomethionine (SO, dietary Se level: 0.7 ppm) over a 6-month period prior to spawning. In Se-supplemented groups, the total number of females spawning was significantly higher compared to the negative control group and the females fed SO began to spawn earlier compared to females fed SS or NC. Total Se concentrations were significantly higher in female muscle of SO group. Higher Se concentrations in the oocytes of both Se-supplemented groups confirmed a maternal transfer of Se, while total Se concentrations in milt samples were not significantly different between dietary groups. There was no effect on glutathione peroxidase (GPX) or other antioxidant enzyme activity in the liver of female or male broodstock, whereas in the swim-up fry, GPX activity was significantly higher in both Se-supplemented treatments with the highest activity in the SO group. Se-supplementation enhanced the expression of hepatic SelPa in broodstock males and females along with MsrB1, GPx1a, GPx4a2, CAT, Gclc, Keap1 and MsrB2 in male liver. In swim-up fry, only organic Se-supplementation led to a higher gene expression for SelPa, GPX1a, GPX1b2, CAT and MsrB2. An improvement of the oxidative status in whole-body of swim-up fry could not be confirmed in this study as the GSH/GSSG was even lower in swim-up fry of Se-supplemented groups, whereas the 8-isoprostane levels were lower in the SS group and no effect on protein carbonyl levels could be detected. Organic Se-supplementation led to a significant increase in the α-tocopherol and vitamin C levels in the progeny. These results show that dietary Se in broodstock nutrition influences spawning occurrence and along with the parental transfer, several traits in the progeny are affected.

Published
2019

8. Developing Feeds with Very Low Levels of Fish Meal and Fish Oil: Issues and Efforts Made as Part of Arraina

Author

Kaushik, Sadasivam, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Universidad de las Palmas de Gran Canaria (ULPGC), and European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012)

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2018

9. Long-Term Dietary Replacement of Fishmeal and Fish Oil in Diets for Rainbow Trout (Oncorhynchus Mykiss): Effects on Growth, Whole Body Fatty Acids and Intestinal and Hepatic Gene Expression

Author

Geneviève Corraze, Françoise Médale, Laurence Larroquet, Viviana Lazzarotto, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0301 basic medicine, Trout, Microarrays, Gene Expression, lcsh:Medicine, Biochemistry, No keyword, Medicine and Health Sciences, farine de poisson, Intestinal Mucosa, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Oligonucleotide Array Sequence Analysis, Animal biology, chemistry.chemical_classification, Multidisciplinary, acide gras, biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, Fatty Acids, Eukaryota, 04 agricultural and veterinary sciences, Fish oil, Lipids, Bioassays and Physiological Analysis, Cholesterol, Liver, Osteichthyes, Oncorhynchus mykiss, Vertebrates, Anatomy, expression des gènes, Research Article, Polyunsaturated fatty acid, alimentation animale, Fish Biology, Carbohydrate metabolism, performance de croissance, Research and Analysis Methods, Real-Time Polymerase Chain Reaction, protéine végétale, 03 medical and health sciences, Fish Oils, Fish meal, Animal science, Fish physiology, Biologie animale, Genetics, Fish Physiology, Animals, Animal Physiology, nutrition animale, 14. Life underwater, Nutrition, lcsh:R, Organisms, Biology and Life Sciences, Fatty acid, remplacement d'aliment, Lipid Metabolism, biology.organism_classification, Animal Feed, Vertebrate Physiology, Diet, Gastrointestinal Tract, Fish, 030104 developmental biology, chemistry, 040102 fisheries, 0401 agriculture, forestry, and fisheries, lcsh:Q, Rainbow trout, Energy Metabolism, Transcriptome, Digestive System, Zoology, huile de poisson
Abstract

International audience; The effects of replacing fishmeal and fish oil with a plant-based diet were studied in juvenile (10g) and ongrowing (250-350g) rainbow trout from first-feeding. Feed-related differences in the intestinal and hepatic transcriptome were examined in juveniles after 7 months of feeding at 7ÊC. Based on microarray results obtained for juveniles, the expression of selected genes related to lipid, cholesterol and energy metabolisms, was assessed by RTqPCR in ongrowing trout after 6 additional months of feeding at 17ÊC. Plasma glucose and cholesterol, lipid content and fatty acid profile of whole body were analyzed at both stages. After 7 months at 7ÊC, all juveniles reached the same body weight (10g), while at 13 months ongrowing fish fed the totally plant-based diet exhibited lower body weight (234 vs 330- 337g). Body lipid content was higher in juveniles fed the totally plant-based diet (13.2 vs 9.4±9.9%), and plasma cholesterol was about 2-times lower in trout fed the plant-based diets at both stages. Fatty acid profile mirrored that of the respective diet, with low proportions of long-chain n-3 polyunsaturated fatty acids in fish fed plant-based diets. Genes involved in protein catabolism, carbohydrate metabolism and trafficking were down-regulated in the intestines of juveniles fed the plant-based diets. This was not true for ongrowing fish. Genes involved in lipid and cholesterol metabolisms were up-regulated in the livers of fish fed plant-based diets for both stages. In this study, feeding trout a totally plant-based diet from first-feeding affect a relatively low proportion of metabolism-related genes. In the longer term, when fish were reared at a higher temperature, only some of these changes were maintained (i.e. up-regulation of lipid/cholesterol metabolism). Although the plant-based diets tested in this study had no major deficiencies, small adjustments in the feed-formula are needed to further optimize growth performance while sparing marine resources.

Published
2018

10. L’alimentation et les maladies d’origine alimentaire

Author

Kaushik, Sadasivam, de Kinkelin, Pierre, Unité de recherche Virologie et Immunologie Moléculaires (VIM (UR 0892)), Institut National de la Recherche Agronomique (INRA), Christian Michel, ProdInra, Migration, Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
[SDV] Life Sciences [q-bio], Santé animale, [SDV]Life Sciences [q-bio], ichtyopathologie, [INFO]Computer Science [cs]
Abstract

Complément numérique; National audience

Published
2018

11. Nutritional History Does Not Modulate Hepatic Oxidative Status of European Sea Bass (Dicentrarchus Labrax) Submitted to Handling Stress

Author

Carolina Castro, Helena Peres, Aires Oliva Teles, Geneviève Corraze, Paula Enes, Amalia Pérez-Jiménez, Filipe Coutinho, Stéphane Panserat, Department of Biology, Faculty of Sciences, University Autonoma of Madrid, Interdisciplinary Centre of Marine and Environmental research (CIIMAR), Centro Interdisciplinar de Investigaçao Marinha e Ambiental (CIIMA), Faculdade de Ciencias, Universidade de Lisboa (ULISBOA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), This work was partially supported by the FCT (Foundation for Science and Technology), Portugal (project PTDC/MAR-BIO/4107/2012) and co-financed by the European Regional Development Fund (ERDF) through the COMPETE - Operational Competitiveness Programme and national funds through FCT, under the project 'PEst-C/MAR/LA0015/2011'. C. Castro, A. Peréz-Jiménez, F. Coutinho, and P. Enes were supported by grants (SFRH/BD/76297/2011, SFRH/BPD/64684/ 2009, SFRH/BD/86799/2012, and SFRH/BPD/101012/2014, respectively) from FCT

Subjects
0301 basic medicine, Physiology, Glutathione reductase, Glucosephosphate Dehydrogenase, Aquatic Science, Biology, medicine.disease_cause, Biochemistry, Lipid peroxidation, 03 medical and health sciences, chemistry.chemical_compound, No keyword, Fish Oils, Dietary Carbohydrates, medicine, Animals, Plant Oils, 14. Life underwater, Food science, Sea bass, ComputingMilieux_MISCELLANEOUS, chemistry.chemical_classification, Glutathione Peroxidase, Superoxide Dismutase, Glutathione peroxidase, [SDV.BA]Life Sciences [q-bio]/Animal biology, 04 agricultural and veterinary sciences, General Medicine, Glutathione, Catalase, Fish oil, biology.organism_classification, Dietary Fats, Oxidative Stress, Glutathione Reductase, 030104 developmental biology, Liver, chemistry, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Bass, Dicentrarchus, Lipid Peroxidation, Oxidative stress
Abstract

International audience; The aim of the present study was to assess the impact of an acute handling stress on hepatic oxidative status of European sea bass (Dicentrarchus labrax) juveniles fed diets differing in lipid so urce and carbohydrate content. For that purpose, four diets were formulated with fish oil (FO) and vegetable oils (VO) as lipid source and with 20 or 0% gelatinized starch as carbohydrate source. Triplicate groups of fish with 74 g were fed each diet during 13 weeks and then subjected to an acute handling stress. Stress exposure decreased hematocrit (Ht) and hemoglobin (Hb) levels. Independent of dietary treatment, stress exposure increased hepatic lipid peroxidation (LPO). Stressed fish exhibited lower glucose 6-phosphate dehydrogenase (G6PD), catalase (CAT), and superoxide dismutase (SOD) activities, independent of previous nutritional history. In the VO groups, stress exposure increased glutathione peroxidase (GPX) activity. Diet composition had no effect on Ht and Hb levels. In contrast, dietary carbohydrate decreased hepatic LPO and CAT activity and increased glutathione reductase (GR) and G6PD activities. Dietary lipids had no effect on LPO. Fish fed the VO diets exhibited higher G6PD activity than fish fed the FO diets. In conclusion, dietary carbohydrates contributed to the reduction of oxidative stress in fish. However, under the imposed handling stress conditions, liver enzymatic antioxidant mechanisms were not enhanced, which may explain the overall increased oxidative stress.

Published
2018

12. Microbiology and immunology of fish larvae

Author

Vadstein, Olav, Bergh, Oivind, Gatesoupe, Francois, Galindo-Villegas, Jorge, Mulero, Victoriano, Picchietti, Simona, Scapigliati, Giuseppe, Makridis, Pavlos, Olsen, Yngvar, Dierckens, Kristof, Defoirdt, Tom, Boon, Nico, De Schryver, Peter, Bossier, Peter, Norwegian Institute of Marine Research, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Department of Cell Biology and Histology, Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA)-University of Amsterdam [Amsterdam] (UvA)-Center for Immunology Amsterdam, Department of Science for Innovative Biology, Agroindustry and Forestry, Tuscia University, Institute of Aquaculture, Laboratory of Aquaculture and Artemia Reference Center, University of Gent, Laboratory of Microbial Ecology and Technology, Universiteit Gent = Ghent University [Belgium] (UGENT), Luis Conceicao, Amos Tandler, Department of Biotechnology, Norwegian University of Science and Technology [Trondheim] (NTNU), Norwegian University of Science and Technology (NTNU)-Norwegian University of Science and Technology (NTNU), Nutrition, Métabolisme, Aquaculture (NUMEA), Institut National de la Recherche Agronomique (INRA), Hellenic Center for Marine Research (HCMR), This work was supported in part by the cost action FAO801 LARVANET: Critical success factors for fish larval production in European Aquaculture: a multidisciplinary network, and by the European FP7 project ‘Promicrobe – Microbes as positive actors for more sustainable aquaculture’ (Project Reference: 227197), and by Research Foundation of Flanders (FWO) project ‘Probiont induced functional responses in aquatic organisms’., ProdInra, Archive Ouverte, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), University of Amsterdam [Amsterdam] (UvA)-Academic Medical Center - Academisch Medisch Centrum [Amsterdam] (AMC), University of Amsterdam [Amsterdam] (UvA)-Center for Immunology Amsterdam, and Ghent University [Belgium] (UGENT)

Subjects
microorganism, animal structures, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.IMM] Life Sciences [q-bio]/Immunology, host-microbe interaction, genetic structures, microbial ecology, poisson, microbiote, piscidin, parasitic diseases, immunologie, microbiologie, [SDV.MP] Life Sciences [q-bio]/Microbiology and Parasitology, fish, [SDV.BA]Life Sciences [q-bio]/Animal biology, fungi, quorum sensing, larve, microbe, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, aquaculture, [SDV.IMM]Life Sciences [q-bio]/Immunology, mast cell, human activities
Abstract

International audience; For most marine aquaculture species, one of the main bottlenecks is the stable production of high quality juveniles. The high and unpredictable mortality in the first weeks after hatching of marine fish larvae remains a challenging problem that needs to be solved. The severity of the problem differs between species, but cannot be considered adequately solved for any species. Both scientific evidence and experience in hatcheries for a variety of fish, shrimp and shellfish species are accumulating as support for the hypothesis that detrimental fish–microbe interactions are the cause of these problems. Host–microbe interactions in reared fish are still poorly understood, except for a few pathogens, and empirical data of the quality required to test this hypothesis, are lacking. This article provides an overview on the current knowledge of the microbial environment of fish larvae, including methodological aspects to characterize the microbial community (both using culture-dependent and culture-independent methods). Further, the current knowledge of the immunology of fish larvae is reviewed, including recent advances in the understanding of toll-like receptors, inflammatory cytokines, mast cells and piscidins, and the ontogeny of the adaptive immune system. Finally, we provide an overview of the state of the art with respect to steering of microbial communities associated with fish larvae – both steering of community composition and of its activity (e.g. by quorum sensing interference)

Published
2018

13. FullSpectrumIsotopic(13)CNMRUsingPolarizationTransferforPosition-Specifi cIsotopeAnalysis

Author

Joubert, Valentin, Silvestre, Virginie, Grand, Mathilde, Loquet, Denis, Ladroue, Virginie, Besacier, Fabrice, Akoka, Serge, Remaud, Gerald S., Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Chimie Et Interdisciplinarité : Synthèse, Analyse, Modélisation (CEISAM), Université de Nantes - Faculté des Sciences et des Techniques, Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Laboratoire de Police Scientifique de Lyon (INPS), Institut National de la Police Scientifique, Laboratoire d'analyse isotopique et électrochimique de metabolismes (LAIEM), Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS), Université de Nantes - UFR des Sciences et des Techniques (UN UFR ST), and Université de Nantes (UN)-Université de Nantes (UN)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)

Subjects
[CHIM]Chemical Sciences
Abstract

International audience; For the last ten years, quantitative isotope ratio monitoring C-13 NMR (irm-C-13 NMR) has been successfully tested and proven as an efficient tool for the determination of position-specific C-13/C-12 ratios. Several applications in different domains have shown the interest in this technique. In the context of origin assignment, the possibility to track the distribution network of illicit drugs or cutting agents is of prime importance. However irm-C-13 NMR still suffers from a relative lack of sensitivity limiting its dissemination among control laboratories. Improvements were proposed to reduce experiment time by using the INEPT sequence (''Insensitive Nuclei Enhanced by Polarization Transfer'') based on polarization transfer from highly sensitive H-1 to less sensitive( 13)C. Several applications based on the use of the one bond scalar coupling between H-1 and C-13 ((1)J(CH)) have shown the potential of this methodology in terms of short experimental duration. However, the isotopic information given by quaternary carbons was lost. The aim of this study is to extend this approach by using short- and long-range coupling ((1)J(CH), (2)J(CH), and (3)J(CH)) in order to have access to all C-13/C-12 position-specific ratios, i.e., acquisition of the full spectrum (FS-INEPT). It is shown that this innovative tool provides both sensitivity gain-thanks to the long-range polarization transfer-and appropriate repeatability. The relative isotopic profiles allowed the classification of two cutting agents, caffeine and paracetamol (acetaminophen), according to their origin, as it was previously observed with ``classical'' irm-C-13 NMR but consuming much less sample and/or reducing the experimental time.

Published
2018

14. Effect of Dietary Methionine Level on Muscle Growth Mechanisms in Juvenile Rainbow Trout (Oncorhynchus Mykiss)

Author

Inge Geurden, Hélène Alami-Durante, Marianne Cluzeaud, Stéphanie Fontagné-Dicharry, Sadasivam Kaushik, Didier Bazin, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), This work was funded under the EU seventh Framework Program by the ARRAINA project No.288925: Advanced Research Initiatives for Nutrition & Aquaculture., European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0301 basic medicine, medicine.medical_specialty, Methionine, Myogenesis, [SDV.BA]Life Sciences [q-bio]/Animal biology, Aquatic Science, Biology, Muscle hypertrophy, 03 medical and health sciences, chemistry.chemical_compound, No keyword, 030104 developmental biology, Fish meal, Endocrinology, chemistry, Internal medicine, medicine, Muscle hyperplasia, Juvenile, MYF5, 14. Life underwater, Cell activation, ComputingMilieux_MISCELLANEOUS
Abstract

International audience; The aim of the study was to analyse the effects of different dietary methionine (Met) concentrations on the mechanisms of fish muscle growth (hyperplasia and hypertrophy) by using cellular and molecular approaches. Three plant-based diets differing by their Met level (MD, Met-deficient; MC, Met-control, corresponding to established Met requirement; MH, Met-high) were fed to rainbow trout juveniles for 12 weeks. Met deficiency led to a reduced somatic growth right from the first 3 weeks of feeding onwards, while feeding the MH-diet led to an increased body growth in the 6 last weeks of feeding. The protein gain of the juveniles increased with dietary Met content. The protein retention efficiency of the MD-fed juveniles was half that of the MC- and MH-fed juveniles which were statistically similar. The changes induced by MD- and MH-feeding at muscle molecular and cellular levels were different. Compared to control, dietary Met deficiency altered the expression of myogenic, muscle growth- and metabolism-related genes by increasing Myf5, MyoD1, Mrf4, Mef2a, βact, CathD, GSTπ and TNFα expressions and decreasing Col1α1 and IGF1 expressions, whereas a high level of dietary Met decreased Pax7a2, Mef2c, βact, CathD, GDH2 and NF-κB expressions. The Met-control fed fish were discrimated according to HGF, Pax7a1 and Pax7a2 expressions. Dietary Met level thus acted on the expression of genes regulating specific transition points of myogenesis, and affected the expression of muscle structural genes and growth factors involved in satellite cell activation and muscle growth. Molecular results suggested that MD-diet favours satellite cell return to quiescence, while MC-diet favours satellite cell activation and MH-diet myogenic differentiation. At cellular level, the muscle total cross-sectional area of the juveniles fed the MD-diet was 28% lower than that of the juveniles fed the MC-diet despite a similar total number of white muscle fibres, and the number of white muscle fibres with a large diameter was lower in MD-fed fish than in MC-fed ones. The muscle total cross-sectional area of the juveniles fed the MH-diet was 32% higher than that of the juveniles fed the MC-diet, their total number of white muscle fibres 25% higher, and their number of white muscle fibres with a large diameter similar. Increasing dietary methionine above established requirement thus promotes white muscle hyperplasia and muscle growth in rainbow trout juveniles, while methionine deficiency dysregulates the expression of myogenic and muscle growth-related genes and impairs white fibre hypertrophy. Statement of relevance: Determining the effects of dietary methionine levels on muscle growth mechanisms will help develop low fish meal feeds.

Published
2018

15. Effects of parental and first-feeding methionine deficiency on rainbow trout (Oncorhynchus mykiss) fry myogenesis

Author

Alami-Durante, Helene, Bazin, Didier, Cluzeaud, Marianne, Héraud, Cécile, Fontagne-Dicharry, Stephanie, Kaushik, Sadasivam J., Geurden, Inge, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Microbiologie du Sol et de l'Environnement (MSE), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Ghent University [Belgium] (UGENT). BEL., and European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012)

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

16. Rainbow trout: long term feeding with low FM and FO feeds including broodstock

Author

Corraze, Geneviève, LAZZAROTTO, Viviana, Kaushik, Sadasivam J., Médale, Françoise, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

17. Parental and Early-Feeding Effects of Dietary Methionine in Rainbow Trout (Oncorhynchus Mykiss)

Author

Stéphanie Fontagné-Dicharry, Cláudia Aragão, Sadasivam Kaushik, Hélène Alami-Durante, Inge Geurden, Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), This work was funded under the EU Seventh Framework Programme by the ARRAINA project No. 288925: Advanced Research Initiatives for Nutrition & Aquaculture. C. Aragão acknowledges financial support through grant SFRH/BPD/108389/2015 (F.C.T., Portugal) co-financed by Fundo Social Europeu (POCH) and National Funds, European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0301 basic medicine, Atlantic salmon, medicine.medical_specialty, Reproductive-performance, Dna methylation, Offspring, Broodstock, Aquatic Science, Biology, Low-density-lipoprotein, 03 medical and health sciences, chemistry.chemical_compound, No keyword, Transcriptional regulation, Fish meal, Animal science, Internal medicine, medicine, Gene-expression, 14. Life underwater, ComputingMilieux_MISCELLANEOUS, S-adenosylmethionine, Methionine, [SDV.BA]Life Sciences [q-bio]/Animal biology, 04 agricultural and veterinary sciences, Metabolism, Cystine level, Fecundity, Spawn (biology), 030104 developmental biology, Endocrinology, chemistry, Protein-turnover, Oxidative stress, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Rainbow trout
Abstract

We studied the effect of changes in dietary methionine (Met) supply in broodstock and first-feeding rainbow trout fry (offspring). Three plant-based diets differing in Met level (deficient, adequate or in excess of the established requirement) were fed to the broodstock (male and female) for 6 months prior to spawning (diets BD, BA and BE, respectively). The offspring from the parental Met-groups was then challenged in turn with the different Met fry-diets (FD, FA and FE, respectively) for 3 weeks from first-feeding. At spawning, females fed diet BD had significantly higher plasma total and LDL-cholesterol and slightly lower plasma triacylglycerol. Diet BD reduced female (but not male) growth, weight of spawn and egg size, but had no effect on relative fecundity. The free amino acid profile of oocytes was modified, with levels of Met and Cys correlating positively with the Met-levels of broodstock diets. SAM and SAH levels in oocytes followed the same pattern, as opposed to SAM/SAH ratio. At the swim-up stage, no significant effect of parental diet on fry weight was noted, whereas survival was the highest in fry from BE-broodstock. The subsequent 21-day fry feeding with different Met levels highly affected the daily growth index with a significant interaction between the parental-diet and fry-diet effects. The expression of a number of genes regulating sulfur amino acid metabolism was modified either directly by the dietary Met supply in both broodstock liver and in whole fry (e.g. BHMT1, GR, GST pi, MsrA1) or indirectly by the parental Met intakes as seen in the swim-up fry (e.g. BHMT1, MTR, GST pi, MsrA1). Importantly, long-lasting parental effects linked to broodstock Met-intake were seen in the fry, 21-days after first-feeding and irrespective of the fry diet (CTH, MsrA1, MsrB2, SOD2). Similarly, parental effects were noted on the gene expression of both NPY and POMC feeding peptides in fry prior to exogenous feeding which persisted for POMC in the 21-day fry. Parental effects were also demonstrated on the key myogenic gene Myog, on fMHC and GDH in swim-up fry, which persisted for GDH in 21-day fry. In summary, our results demonstrate that dietary Met levels of rainbow trout broodstock affect various traits in the offspring, some of which persisted during the first weeks of exogenous feeding. Further studies need to evaluate the long-term persistence of the parental effects over time and to elucidate the mechanisms, whether epigenetic or not. Statement of relevance: Determining the multiple effects of dietary methionine levels on reproductive, growth performance and metabolism in offspring will help improve formulations of low fish meal feeds for rainbow trout at sensitive life cycle stages. (C) 2016 Elsevier B.V. All rights reserved. EU [288925] F.C.T., Portugal - Fundo Social Europeu (POCH) and National Funds [SFRH/BPD/108389/2015] info:eu-repo/semantics/publishedVersion

Published
2017

18. Bases de la nutrition et formulation en aquaculture

Author

BUREL, Christine, Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Raphaëla Le Gouvello, and François Simard

Subjects
fish, nutrition, aquaculture, poisson, alimentation animale, Alimentation et Nutrition, besoin nutritionnel, Food and Nutrition, animal feeding, formulation de l'aliment, nutritional requirement, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Bases de la nutrition et formulation en aquaculture

Published
2017

19. Parental and Early-Feeding Effects of Dietary Methionine on Antioxidant Defence System of Rainbow Trout (Oncorhynchus Mykiss)

Author

Fontagne-Dicharry, Stephanie, Yu, Yingying, Vandermeeren, Emmanuelle, Larroquet, Laurence, Héraud, Cécile, Cluzeaud, Marianne, Alami-Durante, Helene, Kaushik, Sadasivam J., Geurden, Inge, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Microbiologie du Sol et de l'Environnement (MSE), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB), Ghent University [Belgium] (UGENT). Ghent, BEL., and European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012)

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2017

20. Seven Generations of Divergent Selection for Muscle Lipid Content in Rainbow Trout: Main Impacts on Lipid Distribution and Metabolism

Author

Panserat, Stéphane, Bugeon, Jérôme, Plagnes- Juan, Elisabeth, Larroquet, Laurence, Surget, Anne, Kerneis, Thierry, Goardon, Lionel, Médale, Françoise, Lefèvre, Florence, Quillet, Edwige, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and European Aquaculture Society (EAS). BEL.

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

21. Impact of dietary sulphur amino acid deficiency on growth performance, lipid peroxidation and antioxidant status of rainbow trout fed diets with different protein levels

Author

Fontagné-Dicharry, Stéphanie, Larroquet, Laurence, Véron, Vincent, Herman, Alexandre, Alami-Durante, Hélène, Kaushik, Sadasivam J., Geurden, Inge, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), This work was partly funded under the EU seventh Framework Programme by the ARRAINA project N°288925: Advanced Research Initiatives for Nutrition & Aquaculture, European Aquaculture Society (EAS). BEL., European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

22. Effets du remplacement conjoint des farines et huiles de poisson par des ingrédients végétaux chez la truite arc-en-ciel depuis la première alimentation jusqu'à la reproduction

Author

Corraze, Geneviève, LAZZAROTTO, Viviana, Larroquet, Laurence, Médale, Françoise, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA., European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

23. Effect of nutrient availability on progenitor cells in zebrafish (Danio rerio)

Author

Benítez-Santana, Tibiábin, Simion, Matthieu, Orraze, Geneviève, Médale, Françoise, Joly, Jean-Stéphane, Institut des Neurosciences de Paris-Saclay (Neuro-PSI), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS), Department of Internal Medicine, Division of Endocrinology Metabolism, and Diabetes, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Institut des Neurosciences Paris-Saclay (NeuroPSI), Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Benítez-Santana received a support of the European Union, in the framework of the Marie-Curie FP7 COFUND People Programme, through the award of an AgreenSkills fellowship (267196)., and European Project: 267196,EC:FP7:PEOPLE,FP7-PEOPLE-2010-COFUND,AGREENSKILLS(2012)

Subjects
Superior Colliculi, [SDV.NEU.PC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Psychology and behavior, Neurogenesis, Stem Cells, [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology, Neuroepithelial Cells, [SDV.NEU.SC]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Cognitive Sciences, zebrafish, neural stem cell, Neural Stem Cells, Starvation, Ependyma, Models, Animal, Animals, Animal Nutritional Physiological Phenomena, optic tectum, nutritional deprivation, Cell Proliferation
Abstract

In zebrafish brains, populations of continuously proliferating cells are present during an entire life span. Under normal conditions, stem cells give rise to rapidly proliferating progenitors that quickly exit the cell cycle and differentiate. Hence fish are favorable models to study what regulates postembryonic neurogenesis. The aim of this study was to determine if optic tectum (OT) cell proliferation is halted during nutritional deprivation (ND) and whether or not it can be restored with refeeding. We examined the effect of ND on the proliferation of Neuroepithelial/Ependymal Progenitor cell (NeEPC) and transitory-amplifying progenitors (TAPs). Following ND, no PCNA immunostaining was found in OT of starved fish, while positive cell populations of PCNA positive progenitors are found at its periphery in control fish. This indicated that active proliferation stopped. To label retaining progenitor cells, BrdU was applied and a chase-period was accompanied by ND. Positive NeEPCs were detected in the external tectum marginal zone of starved fish suggesting that these progenitors are relatively immune to ND. Moreover in the internal tectum marginal zone labeled retaining cells were observed leaving the possibility that some arrested TAPs were present to readily restart proliferation when nutrition was returned. Our results suggest that neurogenesis was maintained during ND and that a normal proliferative situation was recovered after refeeding. We point to the mTOR pathway as a necessary pathway in progenitors to regulate their mitosis activity. Thus, this study highlights mechanisms involved in neural stem and progenitor cell homeostatic maintenance in an adverse situation. © 2016 Wiley Periodicals, Inc. Develop Neurobiol 77: 26-38, 2017.

Published
2016

24. Does broodstock nutritional history affect the response of progeny to different first-feeding diets? A whole-body transcriptomic study of rainbow trout alevins

Author

Geneviève Corraze, Françoise Médale, Laurence Larroquet, David Mazurais, Viviana Lazzarotto, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Université de Brest (UBO)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), This work was supported by the European Advanced Research Initiatives for Nutrition and Aquaculture no. 288925 project and by the ‘Region Aquitaine’, European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), and Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne)

Subjects
0301 basic medicine, V diet experimentaly 100 % plant-based diet, NL neutral lipid, Microarrays, Medicine (miscellaneous), Early stages, Aquaculture, Broodstock, GCK glucokinase, Epigenesis, Genetic, C diet commercial-like FM-FO and plant-based diet, Transcriptome, Pregnancy, FM fishmeal, 2. Zero hunger, chemistry.chemical_classification, FA fatty acid, LC-PUFA long chain PUFA, Nutrition and Dietetics, Diet, Vegetarian, Muscles, PL polar lipid, 04 agricultural and veterinary sciences, Plants, Fish oil, GO gene ontology, Trout, nutrition, Biochemistry, Oncorhynchus mykiss, Animal Nutritional Physiological Phenomena, Female, Dietary Proteins, FO fish oil, Nutritional Status, Carbohydrate metabolism, Biology, M diet marine FM-FO-based diet, fatty acids, 03 medical and health sciences, Animal science, Fish meal, Dietary Carbohydrates, Animals, Fatty acids, COM commercial diet, Nutrition, Plant products, ACL, Body Weight, Fatty acid, Maternal Nutritional Physiological Phenomena, biology.organism_classification, Animal Feed, Dietary Fats, cRNA complementary RNA, 030104 developmental biology, Fish, Animals, Newborn, chemistry, Cy3 Cyanine 3-CTP, VEG plant-based diet, FC fold change, 040102 fisheries, 0401 agriculture, forestry, and fisheries, Rainbow trout, Gene expression, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Energy Metabolism
Abstract

The whole-body transcriptome of trout alevins was characterised to investigate the effects of long-term feeding of rainbow trout broodstock females a diet free of fishmeal and fish oil on the metabolic capacities of progeny. Effects were studied before first feeding and after 3 weeks of feeding diets containing different proportions of marine and plant ingredients. Feeding alevins plant-based diets resulted in lower fish body weight, irrespective of maternal nutritional history. No differences in whole-body lipids were found between treatments, and the tissue fatty acid profile strongly reflected that of the respective broodstock or first-feeding diets. We showed that the maternal diet history did not significantly affect expressions of any genes before the first feeding. Interestingly, we found an effect of maternal nutritional history on gene expression in alevins after 3 weeks of feeding. The major differences in the transcriptome of alevins from plant-based diet-fed females compared with those from commercial-fed females were as follows: (i) down-regulation of genes involved in muscle growth/contraction and (ii) up-regulation of genes involved in carbohydrate and energy metabolism related to the delay in growth/development observed with plant-based diets. Our findings also showed an effect of the first-feeding diets, irrespective of maternal nutritional history. Specifically, the introduction of plant ingredients resulted in the up-regulation of genes involved in amino acid/protein and cholesterol metabolism and in differences in the expressions of genes related to carbohydrate metabolism. Information gained through this study opens up avenues for further reduction of marine ingredients in trout diets, including the whole rearing cycle.

Published
2016

25. Rôle de la méthionine dans la régulation épigénétique de la croissance musculaire chez la truite arc-en-ciel

Author

Seiliez, Iban, Froehlich, J.M., Marandel, Lucie, Gabillard, Jean-Charles, Biga, P.R., Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), University of Alabama at Birmingham [ Birmingham] (UAB), Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), and ProdInra, Archive Ouverte

Subjects
fish, trout, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, méthionine, régulation epigénétique, histone, myogénèse, poisson, rainbow, animal nutrition, nutrition animale, méthylation, croissance musculaire, truite arc en ciel, expression des gènes
Abstract

Crédit Incitatif 2011. Champ thématique : Ressources, Production; Rôle de la méthionine dans la régulation épigénétique de la croissance musculaire chez la truite arc-en-ciel. Journées d’Animation des Crédits Incitatifs du Département de Physiologie Animale et Systèmes d’Elevage (JACI Phase 2016)

Published
2016

26. Impacts de facteurs alimentaires sur le fonctionnement du tractus digestif chez la truite arc-en-ciel : indicateurs moléculaires et biochimiques

Author

Borey, Marion, Panserat, Stéphane, Plagnes- Juan, Elisabeth, Véron, Vincent, Lauga, Béatrice, Burel, Christine, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Métabolisme, Aquaculture (NuMéA), and Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA)

Subjects
fish, protéine végétale, physiologie digestive, plant protein, poisson, animal nutrition, enzymology, [SDV.BA]Life Sciences [q-bio]/Animal biology, nutrition animale, approche génomique, enzymologie, tractus gastrointestinal
Abstract

Crédit Incitatif 2013. Champs thématiques : Ressources, Production; Impacts de facteurs alimentaires sur le fonctionnement du tractus digestif chez la truite arc-en-ciel : indicateurs moléculaires et biochimiques. Journées d’Animation des Crédits Incitatifs du Département de Physiologie Animale et Systèmes d’Elevage (JACI Phase 2016)

Published
2016

27. Essai de manipulation de la voie NOS par l'alimentation précoce chez la truite : conséquences sur l'expression de gènes myogéniques

Author

Alami-Durante, Hélène, Fonseca, Mélina, Cluzeaud, Marianne, Kaushik, Sadasivam, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), and ProdInra, Archive Ouverte

Subjects
fish, [SDV.BA] Life Sciences [q-bio]/Animal biology, poisson, animal nutrition, growth, [SDV.BA]Life Sciences [q-bio]/Animal biology, arginine, nutrition animale, myogénèse, croissance, expression des gènes
Abstract

Crédit Incitatif 2012. Champ thématique : Production; Essai de manipulation de la voie NOS par l'alimentation précoce chez la truite : conséquences sur l'expression de gènes myogéniques. Journées d’Animation des Crédits Incitatifs du Département de Physiologie Animale et Systèmes d’Elevage (JACI Phase 2016)

Published
2016

28. Première approche épigénétique en nutrition des poissons : étude de la régulation épigénétique de la néogluconéogénèse hépatique chez la truite arc-en-ciel (Oncorhynchus mykiss) - conséquences d'un régime hyperglucidique

Author

Marandel, Lucie, Panserat, Stephane, Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and ProdInra, Archive Ouverte

Subjects
fish, trout, oncorhynchus mykiss, [SDV.BA] Life Sciences [q-bio]/Animal biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, néoglucogenèse, régulation epigénétique, rainbow trout, glucide, épigénétique, poisson, rainbow, animal nutrition, gène dupliqué, nutrition animale, duplicate genes, truite arc en ciel
Abstract

Crédit Incitatif 2014. Champ thématique : Ressources, Adaptation; Première approche épigénétique en nutrition des poissons : étude de la régulation épigénétique de la néogluconéogénèse hépatique chez la truite arc-en-ciel (Oncorhynchus mykiss) - conséquences d'un régime hyperglucidique. Journées d’Animation des Crédits Incitatifs du Département de Physiologie Animale et Systèmes d’Elevage (JACI Phase 2016)

Published
2016

29. Rôle de l'autophagie hépatique dans l'utilisation du glucose chez la truite

Author

Seiliez, Iban, Belghit, Ikram, Gao, Yujie, Skiba-Cassy, Sandrine, Dias, Karine, Cluzeaud, Marianne, Remond, Didier, Hafnaoui, Noureddine, Salin, B., Camougrand, Nadine, Panserat, Stephane, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, IBGC UMR 2095, Centre National de la Recherche Scientifique (CNRS), Université de Bordeaux, Service Commun de Microscopie, Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), ProdInra, Archive Ouverte, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
fish, autophagy, trout, [SDV.BA] Life Sciences [q-bio]/Animal biology, poisson, animal nutrition, [SDV.BA]Life Sciences [q-bio]/Animal biology, autophagie, nutrition animale, truite, glucose, métabolisme, colchicine
Abstract

Crédit Incitatif 2013. Champ thématique : RessourcesCrédit Incitatif 2013. Champ thématique : Ressources; Rôle de l'autophagie hépatique dans l'utilisation du glucose chez la truite. Journées d’Animation des Crédits Incitatifs du Département de Physiologie Animale et Systèmes d’Elevage (JACI Phase 2016)

Published
2016

30. Influence de la teneur en lipides alimentaires sur les mécanismes de 'lipid sensing' au niveau central (hypothalamus) et périphérique (foie) chez la truite arc-en-ciel en lien avec la régulation de la prise alimentaire

Author

Libran-Perez, Marta, Geurden, Inge, Corraze, Geneviève, Panserat, Stéphane, Soengas, Jose Luis, Universidade de Vigo, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), ProdInra, Archive Ouverte, and Universidate de Vigo

Subjects
fish, oncorhynchus mykiss, [SDV.BA] Life Sciences [q-bio]/Animal biology, poisson, lipid, animal nutrition, signalisation cellulaire, [SDV.BA]Life Sciences [q-bio]/Animal biology, cell signalling, nutrition animale, prise alimentaire, rainbow trout, lipide
Abstract

Crédit Incitatif 2014. Champ thématique : Adaptation; Influence de la teneur en lipides alimentaires sur les mécanismes de "lipid sensing" au niveau central (hypothalamus) et périphérique (foie) chez la truite arc-en-ciel en lien avec la régulation de la prise alimentaire. Journées d’Animation des Crédits Incitatifs du Département de Physiologie Animale et Systèmes d’Elevage (JACI Phase 2016)

Published
2016

31. Conséquences à long-terme d'une alimentation à base de matières premières végétales sur la régulation du métabolisme énergétique et lipidique chez la truite arc-en-ciel : focus particulier sur les effets trans-générationnels et les stades précoces

Author

LAZZAROTTO, Viviana, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Région Aquitaine / Projet européen Arraina, Université de Pau et des Pays de l'Adour, Françoise Médale, European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
[SDV.BA]Life Sciences [q-bio]/Animal biology, these
Abstract

In the last years, the increase in aquaculture production has forced a change in fish feed composition, with increasing substitution of fish meal and fish oil by more available plant sources. In this context, the present PhD work aimed at analyzing the effects of different levels of concomitant dietary replacement of fish meal and fish oil by plant ingredients during the whole life cycle of rainbow trout (from first feeding to reproduction). This work showed for the first time that rainbow trout was able to survive, grow and produce viable offsprings, when fed a totally plant-based diet throughout the whole life cycle. Such dietary replacement resulted in drastic changes in tissues fatty acid profile of broodstock females. However, we found that trout was capable to synthetize n-3 long chain- polyunsaturated fatty acids (LC-PUFA) from dietary precursors and to transfer large amounts of these fatty acids in ova, so that an efficient reproduction can occur. We also studied the potential carry over generation effects of feeding broodstock a totally plantbased diet on progeny and their response to different diets at early stages, using molecular approaches, including the study of the whole body transcriptome. Considering the effects on progeny, the present study confirmed the capability of trout to survive and grow on a plant-based diet, but with slight differences in terms of weight. While no effects of maternal dietary background were observed before first feeding, except slightly lower body weight (-13%), significant effects on the transcriptome of whole body alevins appeared after 3 weeks of feeding. These effects of maternal nutritional history were mainly related to muscle growth/contraction and carbohydrate and energy metabolism. Irrespective of the maternal origin of progeny, first feeding diets containing plant ingredients resulted in up-regulation of genes involved in AA/protein and cholesterol metabolism, as well as in changes in the expression of genes involved in carbohydrate metabolism. Finally, the effects of long-term feeding trout plant-based diets were also studied in juveniles (10g) and ongrowing fish (250-350g), mainly focusing on intestine and liver gene expression. The long term trial in juveniles highlighted subtle effects on both intestinal and hepatic gene expression (transcriptome), mainly related to LC-PUFA and cholesterol biosynthetic pathways,which were enhanced in fish fed the plant based diets. This transcriptional pattern was maintained in ongrowing fish. Genes involved in protein catabolism, carbohydrate metabolism and trafficking were also affected by plant-based diets in juveniles, but these results were not fully confirmed in ongrowing fish. Overall, the results of the present thesis allowed extending the use of diets with high replacement rates of fish meal and fish oil to the whole life cycle of fish, including broodstock and early stages. Adjustments of the feed-formula are still needed to further optimize reproductive and growth performance.; Au cours des dernières années, l'augmentation de la production aquacole a nécessité un changement dans la composition des aliments pour poissons, pour remplacer la farine et l’huile de poisson par des sources végétales plus disponibles. Dans ce contexte, le travail de ma thèse avait pour but d'analyser les effets d’une substitution totale et concomitante de la farine et l'huile de poisson par des ingrédients végétaux dans les aliments distribués pendant tout le cycle de vie de la truite arc-en-ciel, de la première alimentation jusqu’à la reproduction. Ce travail a montré pour la première fois que la truite arc-en-ciel peut survivre, se développer et avoir une descendance viable, lorsqu’elle est nourrie tout au long de son cycle de vie avec un régime 100% végétal. Un tel remplacement alimentaire a entraîné des changements radicaux dans le profil en acides gras des tissus des femelles. Cependant, nous avons constaté que la truite était capable de synthétiser des acides gras polyinsaturés à longue chaîne n-3 (AGPI-LC n-3) à partir de précurseurs présents dans l'aliment, et de stocker dans les ovules une partie de ces acides gras néosynthétisés dans le foie , permettant ainsi une reproduction efficace. Nous avons également étudié les possibles effets trans-générationnels d’une alimentation 100% végétale des géniteurs, sur la survie et la croissance de la descendance et sur sa réponse à différents régimes aux stades précoces en nous appuyant sur des analyses du transcriptome des alevins entiers. Aucun effet significatif de l’alimentation maternelle avec des régimes à base de végétaux n’a été observé sur les descendants avant leur première alimentation, à l'exception d'un poids corporel légèrement inférieur (-13%) à celui des descendants de femelles nourries avec un aliment contenant des farines et huiles de poisson. En revanche, des effets significatifs de l'alimentation maternelle sur le transcriptome des alevins sont apparus après 3 semaines d'alimentation. L'histoire nutritionnelle de la mère a affecté principalement des gènes impliqués dans la croissance/contraction musculaire et dans les métabolismes énergétique et glucidique. Quelle que soit l'origine maternelle, l’alimentation des alevins avec des aliments contenant des ingrédients végétaux conduit à une régulation positive des gènes impliqués dans le métabolisme des AA/protéines et le métabolisme du cholestérol, ainsi qu’à des changements dans l'expression des gènes impliqués dans le métabolisme des glucides. Enfin, les effets à long-terme de régimes à base de matières premières végétales chez la truite arc-en-ciel ont également été étudiés chez les juvéniles (10g) et les poissons « en croissance » (250-350g), en se focalisant principalement sur l’expression des gènes dans l’intestin et le foie. Cette étude à long-terme a mis en évidence chez les juvéniles des effets subtils sur des gènes intestinaux et hépatiques (transcriptome), avec principalement une augmentation de l’expression des gènes impliqués dans les voies de synthèse des AGPI-LC et du cholestérol chez les poissons nourris avec des régimes à base de plantes. Ces changements d’expression ont été maintenus chez les poissons « en croissance ». L'expression de gènes impliqués dans le catabolisme des protéines, le métabolisme des glucides et dans le transport intracellulaire a également été modifiée par les régimes à base de plantes chez les juvéniles, mais ces résultats ne sont pas entièrement confirmés chez les poissons « en croissance ». L'ensemble des résultats de cette thèse fournissent des informations originales sur l'utilisation de régimes alimentaires avec des taux de remplacement élevés de farine et d'huile de poisson pendant l'ensemble du cycle de vie des poissons, y compris les reproducteurs et les stades précoces, car la majorité des études précédentes avaient concerné la phase de grossissement. Des ajustements de la formule des aliments à base de végétaux sont encore nécessaires pour continuer à optimiser les performances de reproduction et surtout la croissance des poissons d’élevage.

Published
2016

32. Dietary lipid and carbohydrate interactions: implications on lipid and glucose absorption, transport in gilthead sea bream (Sparus aurata) juveniles

Author

Carolina Castro, Aires Oliva-Teles, Geneviève Corraze, Ana Basto, Stéphane Panserat, Laurence Larroquet, Faculdade de Ciencias, Universidade de Lisboa (ULISBOA), Centro Interdisciplinar de Investigaçao Marinha e Ambiental (CIIMA), Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), This work was partially supported by national funds through the FCT (Foundation for Science and Technology)— under the project‘PEst-C/MAR/LA0015/2011’ and co-financed by the European Regional Development Fund (ERDF) through the COMPETE— operational competitiveness programme. C. C. was supported by a grant (SFRH/BD/76297/2011) from FCT, Portugal, and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0301 basic medicine, nutrient absorption and transport, Biochemistry, Intestinal absorption, chemistry.chemical_compound, serum metabolite, poisson, alternative ingredient, [SDV.BA]Life Sciences [q-bio]/Animal biology, Starch, 04 agricultural and veterinary sciences, Fish oil, Cholesterol, juvénile, Digestion, lipids (amino acids, peptides, and proteins), animal feeding, expression des gènes, Genetic Markers, medicine.medical_specialty, digestibilité, alimentation animale, Dietary lipid, Phospholipid, Biology, sparus aurata, 03 medical and health sciences, lipid, Internal medicine, medicine, Dietary Carbohydrates, Animals, Plant Oils, Lipid Transport, lipide, fish, Organic Chemistry, Cholesterol, HDL, Lipid metabolism, Cell Biology, Cholesterol, LDL, Carbohydrate, Lipid Metabolism, Dietary Fats, Sea Bream, glucide, 030104 developmental biology, Endocrinology, Glucose, chemistry, Gene Expression Regulation, Intestinal Absorption, digestibility, 040102 fisheries, gene expression, 0401 agriculture, forestry, and fisheries, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

A digestibility trial was performed with gilthead sea bream juveniles (IBW = 72 g) fed four diets differing in lipid source (fish oil, FO; or a blend of vegetable oil, VO) and starch content (0 %, CH−; or 20 %, CH+) to evaluate the potential interactive effects between carbohydrates and VO on the processes involved in digestion, absorption and transport of lipids and glucose. In fish fed VO diets a decrease in lipid digestibility and in cholesterol (C), High Density Lipoprotein(HDL)-C and Low Density Lipoprotein (LDL)-C (only in CH+ group) were recorded. Contrarily, dietary starch induced postprandial hyperglycemia and time related alterations on serum triacylglycerol (TAG), phospholipid (PL) and C concentrations. Fish fed a CH+ diet presented lower serum TAG than CH− group at 6 h post-feeding, and the reverse was observed at 12 h post-feeding for TAG and PL. Lower serum C and PL at 6 h post-feeding were recorded only in VOCH+ group. No differences between groups were observed in hepatic and intestinal transcript levels of proteins involved in lipid transport and hydrolysis (FABP, DGAT, GPAT, MTP, LPL, LCAT). Lower transcript levels of proteins related to lipid transport (ApoB, ApoA1, FABP2) were observed in the intestine of fish fed the CH+ diet, but remained unchanged in the liver. Overall, transcriptional mechanisms involved in lipid transport and absorption were not linked to changes in lipid serum and digestibility. Dietary starch affected lipid absorption and transport, probably due to a delay in lipid absorption. This study suggests that a combination of dietary VO and starch may negatively affect cholesterol absorption and transport.

Published
2016

33. Molecular pathways associated with the nutritional programming of plant-based diet acceptance in rainbow trout following an early feeding exposure

Author

Mukundh Balasubramanian, Mathilde Dupont-Nivet, Françoise Médale, Sadasivam Kaushik, Jérôme Montfort, Inge Geurden, Edwige Quillet, Stéphane Panserat, Aurélie Le Cam, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), This work was part of a Postdoctoral fellowship programme to MNB funded by INRA, under the INRA-WUR Aquaculture platform. The study was partly funded by INRA (Department Phase, Crédit Incitatif), by the VEGEAQUA project (FUI funding, French government) and by the EU-funded ARRAINA project No. 288925: Advanced Research Initiatives for Nutrition & Aquaculture (EU Seventh Framework Programme), European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique )-Institut National de la Recherche Agronomique (INRA), European Project: 288925, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), and AgroParisTech-Institut National de la Recherche Agronomique (INRA)

Subjects
0301 basic medicine, alimentation animale, Animal feed, animal diseases, mécanisme moléculaire, Transcriptome, 03 medical and health sciences, Animal science, poisson, Aquaculture, Biologie animale, Genetics, Animals, Food and Nutrition, Juvenile, Gene Regulatory Networks, 14. Life underwater, Genetic Association Studies, Animal biology, oncorhynchus mykiss, biology, business.industry, Gene Expression Profiling, transcriptomique, [SDV.BA]Life Sciences [q-bio]/Animal biology, Computational Biology, Reproducibility of Results, programmation nutritionnelle, biology.organism_classification, Animal Feed, Biotechnology, Trout, Gene Ontology, juvénile, 030104 developmental biology, Gene Expression Regulation, Organ Specificity, alimentation végétale, Alimentation et Nutrition, Animal Nutritional Physiological Phenomena, Rainbow trout, business, Oviparity, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Research Article
Abstract

Background The achievement of sustainable feeding practices in aquaculture by reducing the reliance on wild-captured fish, via replacement of fish-based feed with plant-based feed, is impeded by the poor growth response seen in fish fed high levels of plant ingredients. Our recent strategy to nutritionally program rainbow trout by early short-term exposure to a plant-based (V) diet versus a control fish-based (M) diet at the first-feeding fry stage when the trout fry start to consume exogenous feed, resulted in remarkable improvements in feed intake, growth and feed utilization when the same fish were challenged with the diet V (V-challenge) at the juvenile stage, several months following initial exposure. We employed microarray expression analysis at the first-feeding and juvenile stages to deduce the mechanisms associated with the nutritional programming of plant-based feed acceptance in trout. Results Transcriptomic analysis was performed on rainbow trout whole fry after 3 weeks exposure to either diet V or diet M at the first feeding stage (3-week), and in the whole brain and liver of juvenile trout after a 25 day V-challenge, using a rainbow trout custom oligonucleotide microarray. Overall, 1787 (3-week + Brain) and 924 (3-week + Liver) mRNA probes were affected by the early-feeding exposure. Gene ontology and pathway analysis of the corresponding genes revealed that nutritional programming affects pathways of sensory perception, synaptic transmission, cognitive processes and neuroendocrine peptides in the brain; whereas in the liver, pathways mediating intermediary metabolism, xenobiotic metabolism, proteolysis, and cytoskeletal regulation of cell cycle are affected. These results suggest that the nutritionally programmed enhanced acceptance of a plant-based feed in rainbow trout is driven by probable acquisition of flavour and feed preferences, and reduced sensitivity to changes in hepatic metabolic and stress pathways. Conclusions This study outlines the molecular mechanisms in trout brain and liver that accompany the nutritional programming of plant-based diet acceptance in trout, reinforces the notion of the first-feeding stage in oviparous fish as a critical window for nutritional programming, and provides support for utilizing this strategy to achieve improvements in sustainability of feeding practices in aquaculture. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2804-1) contains supplementary material, which is available to authorized users.

Published
2016

34. Mineral Requirements of Fish: A Systematic Review

Author

P. Antony Jesu Prabhu, Sadasivam Kaushik, Johan W. Schrama, Wageningen Institute of Animal Sciences, Wageningen University and Research [Wageningen] (WUR), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), This work was part of a PhD programme funded by INRA, under the INRA-WUR aquaculture platform. This work is also a contribution to the EU-funded project, under the EU Seventh Framework Programme by the ARRAINA project No. 288925: Advanced Research Initiatives for Nutrition & Aquaculture, European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), Wageningen University and Research Center (WUR), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0301 basic medicine, Mineralogy, Management, Monitoring, Policy and Law, Aquatic Science, 03 medical and health sciences, No keyword, Animal science, Aquaculture and Fisheries, Response criteria, medicine, 14. Life underwater, ComputingMilieux_MISCELLANEOUS, Trace elements, Mineral, Ecology, Chemistry, Aquacultuur en Visserij, [SDV.BA]Life Sciences [q-bio]/Animal biology, Line model, Trace element, 04 agricultural and veterinary sciences, Digestive physiology, Meta-analysis, 030104 developmental biology, Fish, Mineral requirements, 040102 fisheries, WIAS, 0401 agriculture, forestry, and fisheries, %22">Fish , medicine.symptom, Inclusion (mineral), Weight gain
Abstract

International audience; Meta-analysis of literature data on mineral and trace element requirements of fish was performed with the major objectives of identifying appropriate response criteria and the factors affecting the minimal dietary inclusion levels. The primary data set included 25 studies on available P, 20 on Ca, 24 on Mg, 5 on K, 37 for Zn, 23 for Se, 19 for Mn, 16 for Fe and 13 for Cu. Broken line regression analysis with linear plateau model (P, Ca, Mg and K) or two-linear line model (Zn, Cu, Fe, Mn and Se) was used for determining the minimal dietary inclusion levels. Vertebral mineral concentration (P, Ca, Zn and Mn), whole-body mineral levels (Mg, K and Fe) and hepatic enzyme activity (Se) were found to be the most appropriate criteria for the respective minerals analysed. In general, weight gain as the criterion resulted in a lower estimate (by 18–42%) than those obtained using whole-body or vertebral mineral concentrations as response criteria. The analysis also showed that different fish species do not show large variations in the mineral and trace element concentrations in the whole body and tissues. Factors such as species group and digestive physiology, type of experimental diet used and dietary interactions, type of mineral source and mineral concentration of water were found to affect the minimal dietary inclusion levels of certain minerals. Besides the meta-analysis, research needs in mineral nutrition of fish with reference to growing changes in dietary strategies and rearing systems are discussed

Published
2016

35. Qualité de la chair des lignées de truite arc-en-ciel après sept générations de sélection sur la teneur en lipides musculaires

Author

Lefèvre, Florence, Bugeon, Jérôme, Goardon, Lionel, Kerneis, Thierry, Labbé, Laurent, Panserat, Stephane, Médale, Françoise, QUILLET, Edwige, Laboratoire de Physiologie et Génomique des Poissons (LPGP), Institut National de la Recherche Agronomique (INRA)-Structure Fédérative de Recherche en Biologie et Santé de Rennes ( Biosit : Biologie - Santé - Innovation Technologique ), Pisciculture Expérimentale INRA des Monts d'Arrée (PEIMA), Institut National de la Recherche Agronomique (INRA), Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Génétique Animale et Biologie Intégrative (GABI), and Institut National de la Recherche Agronomique (INRA)-AgroParisTech

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology
Abstract

International audience; Flesh lipid content is a determinant of fillet quality that could be controlled by selective breeding. Two lines of rainbow trouts divergently selected for lipid content, a lean muscle line (L) and a fat muscle line (F), were assessed for raw, cooked, and smoked fillet quality parameters. Fish from the L line gave consistently lower values than those from the F line for all adiposity parameters (fat-meter value, muscle lipid and dry matter content). Raw fillet from L fish gave lower values of lightness, redness and yellowness. However, cooked fillets differed only for yellowness whereas smoked fillets differed for both redness and yellowness but not for lightness. Mechanical resistance of fillets was also affected by selection. Fillets from L fish were firmer at slaughter time and two days post-mortem, as were the smoked fillets. However, no difference in textural parameters was measured between cooked fillets from the two lines. Histological measurement of white muscle fiber size revealed that M fish had much smaller fiber than G fish.

Published
2016

36. Parental Effects of Changes in Dietary Methionine Supply in Rainbow Trout

Author

Geurden, Inge, Fontagné-Dicharry, Stéphanie, Alami-Durante, Hélène, Aragao, C., Kaushik, Sadasivam J., Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centro de Ciencias do Mar do Algarve (CIMAR/CCMAR), This work was partly funded under the EU seventh Framework Programme by the ARRAINA project no. 288925 : Advanced Research Initiatives for Nutrition and Aquaculture), European Aquaculture Society (EAS). BEL., and European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012)

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

37. Hepatic fatty acid biosynthesis is more responsive to protein than carbohydrate in rainbow trout during acute stimulations

Author

Weiwei Dai, Elisabeth Plagnes-Juan, Iban Seiliez, Sadasivam Kaushik, Frédéric Terrier, Stéphane Panserat, Sandrine Skiba-Cassy, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and W. Dai gratefully acknowledges the financial assistance provided by the China Scholarship Council (CSC, file no. 2011633111) for his doctoral fellowship

Subjects
0301 basic medicine, target of rapamycin, Time Factors, food intake, ATP citrate lyase, Physiology, fatty acid biosynthesis, rapamycine, biosynthèse, poisson, Insulin, Amino Acids, Cells, Cultured, lipogénèse, chemistry.chemical_classification, trout, oncorhynchus mykiss, alimentation, biology, acide gras, TOR Serine-Threonine Kinases, [SDV.BA]Life Sciences [q-bio]/Animal biology, Fatty Acids, rainbow trout, Pyruvate carboxylase, Fatty acid synthase, sirolimus, Biochemistry, protéine, Lipogenesis, Animal Nutritional Physiological Phenomena, Dietary Proteins, Signal Transduction, Fish Proteins, medicine.medical_specialty, Primary Cell Culture, Nutritional Status, Mechanistic Target of Rapamycin Complex 1, Gene Expression Regulation, Enzymologic, 03 medical and health sciences, rainbow, Physiology (medical), Internal medicine, medicine, Dietary Carbohydrates, Animals, nutrition animale, Protein Kinase Inhibitors, lipogenesis, fish, Fatty acid, Carbohydrate, Sterol regulatory element-binding protein, glucide, 030104 developmental biology, Endocrinology, Enzyme, Glucose, chemistry, carbohydrate, Multiprotein Complexes, animal nutrition, biology.protein, Hepatocytes, fatty acid, biosynthesis, protein, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, truite arc en ciel
Abstract

The link between dietary carbohydrate/protein and de novo lipogenesis (DNL) remains debatable in carnivorous fish. We aimed to evaluate and compare the response of hepatic lipogenic gene expression to dietary carbohydrate intake/glucose and dietary protein intake/amino acids (AAs) during acute stimulations using both in vivo and in vitro approaches. For the in vivo trial, three different diets and a controlled-feeding method were employed to supply fixed amount of dietary protein or carbohydrate in a single meal; for the in vitro trial, primary hepatocytes were stimulated with a low or high level of glucose (3 mM or 20 mM) and a low or high level of AAs (one-fold or four-fold concentrated AAs). In vitro data showed that a high level of AAs upregulated the expression of enzymes involved in DNL [fatty acid synthase (FAS) and ATP citrate lyase (ACLY)], lipid bioconversion [elongation of very long chain fatty acids like-5 (Elovl5), Elovl2, Δ6 fatty acyl desaturase (D6D) and stearoyl-CoA desaturase-1 (SCD1)], NADPH production [glucose-6-phosphate dehydrogenase (G6PDH) and malic enzyme (ME)], and transcriptional factor sterol regulatory element binding protein 1-like, while a high level of glucose only elevated the expression of ME. Data in trout liver also showed that high dietary protein intake induced higher lipogenic gene expression (FAS, ACLY, and Elovl2) regardless of dietary carbohydrate intake, while high carbohydrate intake markedly suppressed the expression of acetyl-CoA carboxylase (ACC) and Elovl5. Overall, we conclude that, unlike rodents or humans, hepatic fatty acid biosynthetic gene expression in rainbow trout is more responsive to dietary protein intake/AAs than dietary carbohydrate intake/glucose during acute stimulations. This discrepancy probably represents one important physiological and metabolic difference between carnivores and omnivores.

Published
2016

38. Using multi-tracer inference to move beyond single-catchment ecohydrology

Author

Paul Romeijn, Clara Mendoza-Lera, Stefan Krause, Anniet M. Laverman, Myrto Nikolakopoulou, Jean-Raynald de Dreuzy, José Leonardo de Moraes Gonçalves, Carolyn Oldham, Tamara Kolbe, Viktor Baranov, Mukundh N. Balasubramanian, Gilles Pinay, Thibault Datry, Benjamin W. Abbott, Timothy N. Vaessen, Astrid Harjung, Audrey Campeau, David M. Hannah, Francesco Ciocca, Marta Antonelli, Marcus B. Wallin, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Leibniz Institute of Freshwater Ecology & Inland Fisheries, Milieux aquatiques, écologie et pollutions (UR MALY), Institut national de recherche en sciences et technologies pour l'environnement et l'agriculture (IRSTEA), Naturalea, University of Barcelona, Géosciences Rennes (GR), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Centre National de la Recherche Scientifique (CNRS), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Centre d'Estudis Avançats de Blanes (CEAB), Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Silixa Ltd., Department of Earth Sciences [ Uppsala], Uppsala University, School of Geography, Earth and Environmental Sciences [Birmingham], University of Birmingham [Birmingham], National Institute of Biology, Milieux Environnementaux, Transferts et Interactions dans les hydrosystèmes et les Sols (METIS), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), Observatoire des Sciences de l'Univers de Rennes (OSUR), EC2CO Grant 'charactérisation hydrologique et biogéochimique de la dénitrification dans les paysages', ONEMA Action 13 'Colmatage, échanges nappe-rivière et processus biogéochimiques', European Project: 607150,EC:FP7:PEOPLE,FP7-PEOPLE-2013-ITN,INTERFACES(2013), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), BioSistemika, Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Department of Earth Sciences [Uppsala], National Institute of Biology [Ljubljana] (NIB), Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), and Université de Rennes (UR)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Rennes 2 (UR2)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
010504 meteorology & atmospheric sciences, 0208 environmental biotechnology, Exposure time, Drainage basin, Inference, Reactive transport, 02 engineering and technology, 01 natural sciences, GW-SWinteractions, Hot spots, Temporal scales, Groundwater, geography.geographical_feature_category, Ecohydrology, Environmental resource management, GW-SW interactions, Surface water, Miljövetenskap, Hydrological tracer, Water, Environmental hydrology, Flowpath, Residence time, Hot moments, Damkohler, Peclet, HotDam, Crossed proxies, Tracer, Aquatic ecology, 570 Biowissenschaften, Biologie, Biogeochemical cycle, [SDE.MCG]Environmental Sciences/Global Changes, Earth and Planetary Sciences(all), Residence time (fluid dynamics), ddc:570, TRACER, Ecosystem, [SDU.STU.HY]Sciences of the Universe [physics]/Earth Sciences/Hydrology, 0105 earth and related environmental sciences, Hydrology, geography, business.industry, Péclet, 15. Life on land, 020801 environmental engineering, Damköhler, 13. Climate action, General Earth and Planetary Sciences, Environmental science, business, Environmental Sciences
Abstract

24 páginas, 9 figuras, 1 tabla., Protecting or restoring aquatic ecosystems in the face of growing anthropogenic pressures requires an understanding of hydrological and biogeochemical functioning across multiple spatial and temporal scales. Recent technological and methodological advances have vastly increased the number and diversity of hydrological, biogeochemical, and ecological tracers available, providing potentially powerful tools to improve understanding of fundamental problems in ecohydrology, notably: 1. Identifying spatially explicit flowpaths, 2. Quantifying water residence time, and 3. Quantifying and localizing biogeochemical transformation. In this review, we synthesize the history of hydrological and biogeochemical theory, summarize modern tracer methods, and discuss how improved understanding of flowpath, residence time, and biogeochemical transformation can help ecohydrology move beyond description of site-specific heterogeneity. We focus on using multiple tracers with contrasting characteristics (crossing proxies) to infer ecosystem functioning across multiple scales. Specifically, we present how crossed proxies could test recent ecohydrological theory, combining the concepts of hotspots and hot moments with the Damköhler number in what we call the HotDam framework., This project has been funded by the European Union's Seventh Framework Program for research, technological development and demonstration under grant agreement no. 607150 (FP7-PEOPLE-2013-ITN – INTERFACES - Ecohydrological interfaces as critical hotspots for transformations of ecosystem exchange fluxes and biogeochemical cycling). B. Abbott and G. Pinay were also supported by the French EC2CO grant “Caractérisation hydrologique et biogéochimique de la dénitrification dans les paysages.” C. Mendoza-Lera and T. Datry were supported by the French National Agency for Water and Aquatic Environments (ONEMA, Action 13, “Colmatage, échanges nappe-rivière et processus biogéochimiques”).

Published
2016

39. Looking at the Metabolic Consequences of the Colchicine-Based in Vivo Autophagic Flux Assay

Author

Marianne Cluzeaud, Ikram Belghit, Sandrine Skiba-Cassy, Didier Rémond, Nadine Camougrand, Nordine Hafnaoui, Bénédicte Salin, Stéphane Panserat, Karine Dias, Yujie Gao, Iban Seiliez, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Institut de biochimie et génétique cellulaires (IBGC), Université Bordeaux Segalen - Bordeaux 2-Centre National de la Recherche Scientifique (CNRS), Service Commun de Microscopie, Université de Bordeaux, This study was supported by the INRA 'Animal Physiology and Livestock Systems' Division, the European Union 7th Framework project (Project Call Identifier: FP7-KBBE-2011-5, Project Number: 288925, Advanced Research Initiatives for Nutrition & Aquaculture (ARRAINA)) and the Conseil Régional d’Aquitaine (CRA n 20121201001) 'Nouvelles stratégies alimentaires pour l’aquaculture' (2013–2015), European Project: 288925,EC:FP7:KBBE,FP7-KBBE-2011-5,ARRAINA(2012), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0301 basic medicine, Fish Proteins, medicine.medical_specialty, Time Factors, Biology, Carbohydrate metabolism, 03 medical and health sciences, chemistry.chemical_compound, No keyword, 0302 clinical medicine, In vivo, Internal medicine, Lipid droplet, Phagosomes, medicine, Autophagy, Colchicine, Animals, Molecular Biology, TOR Serine-Threonine Kinases, [SDV.BA]Life Sciences [q-bio]/Animal biology, Leupeptin, Adenylate Kinase, Lipid metabolism, Cell Biology, Endoplasmic Reticulum Stress, Lipid Metabolism, Basic Research Paper, 3. Good health, Fatty Liver, 030104 developmental biology, Endocrinology, chemistry, Gene Expression Regulation, Liver, Oncorhynchus mykiss, Vacuoles, Carbohydrate Metabolism, Energy Metabolism, Lysosomes, Flux (metabolism), 030217 neurology & neurosurgery, Drug metabolism, Amino Acids, Branched-Chain, Biomarkers
Abstract

International audience; Monitoring autophagic flux in vivo or in organs remains limited and the ideal methods relative to the techniques possible with cell culture may not exist. Recently, a few papers have demonstrated the feasibility of measuring autophagic flux in vivo by intraperitoneal (IP) injection of pharmacological agents (chloroquine, leupeptin, vinblastine, and colchicine). However, the metabolic consequences of the administration of these drugs remain largely unknown. Here, we report that 0.8~mg/kg/day IP colchicine increased LC3-II protein levels in the liver of fasted trout, supporting the usefulness of this drug for studying autophagic flux in vivo in our model organism. This effect was accompanied by a decrease of plasma glucose concentration associated with a fall in the mRNA levels of gluconeogenesis-related genes. Concurrently, triglycerides and lipid droplets content in the liver increased. In contrast, transcript levels of β-oxidation-related gene Cpt1a dropped significantly. Together, these results match with the reported role of autophagy in the regulation of glucose homeostasis and intracellular lipid stores, and highlight the importance of considering these effects when using colchicine as an in vivo "autophagometer."

Published
2016

40. Impact d'une substitution totale de la farine et de \'lhuile de poisson par des végétaux sur le métabolisme du cholesterol de la truite arc-en-ciel (Oncorhynchus mykiss)

Author

Zhu, Tengfei, Corraze, Geneviève, Plagnes-Juan, Élisabeth, Larroquet, Laurence, Dupont-Nivet, Mathilde, QUILLET, Edwige, Skiba, Sandrine, Nutrition, Métabolisme, Aquaculture (NuMéA), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Université Paris-Saclay, and Institut Technique de l'Aviculture et des Elevages de Petits Animaux (ITAVI). FRA.

Subjects
No keyword, [SDV.BA]Life Sciences [q-bio]/Animal biology, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

41. How Tom Moon's research highlighted the question of glucose tolerance in carnivorous fish

Author

Sergio Polakof, Stéphane Panserat, Unité de Nutrition Humaine - Clermont Auvergne (UNH), Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne (UCA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Unité de Nutrition Humaine (UNH), Institut National de la Recherche Agronomique (INRA)-Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université, Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1 (UB)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), and Université d'Auvergne - Clermont-Ferrand I (UdA)-Clermont Université-Institut National de la Recherche Agronomique (INRA)

Subjects
0301 basic medicine, tolérance au glucose, Physiology, glucose metabolism, Fish species, Zoology, hépatocyte, Biology, Carbohydrate metabolism, Biochemistry, 03 medical and health sciences, Predatory fish, poisson, Animals, Humans, 14. Life underwater, nutrition animale, métabolisme du glucose, glucose, Molecular Biology, fish, antidiabetic drug, Meal, business.industry, nutrient, [SDV.BA]Life Sciences [q-bio]/Animal biology, Breaking point, Fishes, Glucose Injection, 04 agricultural and veterinary sciences, Carnivory, Biotechnology, 030104 developmental biology, animal nutrition, 040102 fisheries, 0401 agriculture, forestry, and fisheries, %22">Fish , business, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Fifteen years ago, Tom Moon wrote a review on this journal in order to propose some explanations to the exacerbated glycaemic response after a glucose load or a carbohydrate meal intake observed in fish, the so-called intolerance to glucose. Before, but in most of cases after this paper, several laboratories worldwide started to make important efforts in order to better understand this strange phenotype observed in fish and that so far seemed to belong to diabetic humans only. Tom had been worked on fish metabolism for at least 30years when he proposed that mini-review and the paths opened by him in 2001 were followed by tens of fish researchers, making this paper a breaking point on the field. Fifteen years later, we propose not only to have a look to the answers given to the questions rose in that paper, but also to summarize how his career over all these years impacted the domain of glucose metabolism in fish. In the review, we will show how Tom Moon analysed at different levels (from genes up to the whole organism), using distinct experimental tools (cells, hormone or glucose injection, pumps, drugs) the questions of glucose metabolism, tolerance and nutrition in fish species.

Published
2016

42. Effects of fish oil replacement by vegetable oil blend on digestive enzymes and tissue histomorphology of European sea bass (Dicentrarchus labrax) juveniles

Author

Carolina Castro, Stéphane Panserat, Ana Couto, Geneviève Corraze, Aires Oliva-Teles, Cláudia R. Serra, Amalia Pérez-Jiménez, Patricia Díaz-Rosales, Rui Loja Fernandes, Centro Interdisciplinar de Investigaçao Marinha e Ambiental (CIIMA), Faculdade de Ciencias, Universidade de Lisboa (ULISBOA), Instituto de Biologia Molecular e Celular, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), This work was partially supported by the FCT (Foundation for Science and Technology), Portugal (Project PTDC/MAR-BIO/4107/2012) and co-financed by the European Regional Development Fund (ERDF) through the COMPETE—Operational Competitiveness Programme and national funds through FCT—under the project 'PEst-C/MAR/LA0015/2011'. CC was supported by a Grant (SFRH/BD/76297/2011), AC (SFRH/BD/47495/2008) and APJ (SFRH/BPD/64684/2009) from FCT, Portugal. PDR and CRS were supported by Grants (NORTE-07-0124-FEDER-000038-BPD-2013-07, NORTE-07-0124-FEDER-000038-BPD-2013-05, respectively), and Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA)

Subjects
0301 basic medicine, Hydrolases, Physiology, tissue histology, Palm Oil, Biochemistry, fish oil, bar, Fatty Acids, Monounsaturated, histologie, poisson, Food science, Amylase, huile végétale, biology, [SDV.BA]Life Sciences [q-bio]/Animal biology, 04 agricultural and veterinary sciences, General Medicine, Postprandial Period, Fish oil, Trypsin, vegetal oil, Intestines, Liver, vegetable oil, Alkaline phosphatase, Dicentrarchus, animal feeding, dicentrarchus labrax, sea bass, medicine.drug, Fish Proteins, Linseed Oil, alimentation animale, european sea bass, Aquatic Science, postprandial digestive enzyme, enzyme digestive, 03 medical and health sciences, Fish Oils, tissu, medicine, Animals, Plant Oils, 14. Life underwater, Sea bass, histomorphologie, fish, biology.organism_classification, Dietary Fats, 030104 developmental biology, Vegetable oil, Digestive enzyme, 040102 fisheries, biology.protein, 0401 agriculture, forestry, and fisheries, Bass, Rapeseed Oil, biological tissues, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

The impact of replacing circa 70 % fish oil (FO) by a vegetable oil (VO) blend (rapeseed, linseed, palm oils; 20:50:30) in diets for European sea bass juveniles (IBW 96 ± 0.8 g) was evaluated in terms of activities of digestive enzymes (amylase, lipase, alkaline phosphatase, trypsin and total alkaline proteases) in the anterior (AI) and posterior (PI) intestine and tissue morphology (pyloric caeca—PC, AI, PI, distal intestine—DI and liver). For that purpose, fish were fed the experimental diets for 36 days and then liver and intestine were sampled at 2, 6 and 24 h after the last meal. Alkaline protease characterization was also done in AI and PI at 6 h post-feeding. Dietary VO promoted higher alkaline phosphatase activity at 2 h post-feeding in the AI and at all sampling points in the PI. Total alkaline protease activity was higher at 6 h post-feeding in the PI of fish fed the FO diet. Identical number of bands was observed in zymograms of alkaline proteases of fish fed both diets. No alterations in the histomorphology of PC, AI, PI or DI were noticed in fish fed the VO diets, while in the liver a tendency towards increased hepatocyte vacuolization due to lipid accumulation was observed. Overall, and with the exception of a higher intestine alkaline phosphatase activity, 70 % FO replacement by a VO blend in diets for European sea bass resulted in no distinctive alterations on the postprandial pattern of digestive enzyme activities and intestine histomorphology.

Published
2016

43. Experimental evidence of population differences in reproductive investment conditional on environmental stochasticity

Author

Stéphane Panserat, Zoé Gauthey, Jacques Labonne, Arturo Elosegi, Alexandre Herman, Cédric Tentelier, Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Faculty of Science and technology, University of the Basque Country (University of the Basque Country), This study was funded by the INTERREG Atlantic Aquatic Resource Conservation program (AARC) funded by the European Union, and by the Université de Pau et des Pays de l’Adour via a BQR grant, and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
0106 biological sciences, Sympatry, Water flow, Trout, Climate, Population Dynamics, reproduction animale, salmo trutta, 01 natural sciences, adaptive response, salmonids, Environmental monitoring, Waste Management and Disposal, ComputingMilieux_MISCELLANEOUS, salmonidae, education.field_of_study, changement climatique, Ecology, Reproduction, Investment (macroeconomics), Pollution, Droughts, climate change, réponse adaptative, Female, Seasons, animal ecology, environmental stochasticity, Environmental Monitoring, Environmental Engineering, [SDE.MCG]Environmental Sciences/Global Changes, Population, salmonid, Climate change, ecological contrast, Biology, 010603 evolutionary biology, Environmental Chemistry, Animals, Ecosystem, 14. Life underwater, education, reproductive investment, global change, Life Cycle Stages, sea trout, écologie animale, 010604 marine biology & hydrobiology, 13. Climate action, Animal ecology, stochasticite, [SDE.BE]Environmental Sciences/Biodiversity and Ecology
Abstract

International audience; Environmental stochasticity is expected to shape life histories of species, wherein organisms subjected to strong environmental variation should display adaptive response by being able to tune their reproductive investment. For riverine ecosystems, climate models forecast an increase in the frequency and intensity of extreme events such as floods and droughts. The speed and the mechanisms by which organisms may adapt their reproductive investment are therefore of primary importance to understand how species will cope with such radical environmental changes. In the present study, we sampled spawners fromtwo different populations of wild brown trout, originating from two environments with contrasting levels of flow stochasticity. We placed them in sympatry within an experimental channel during reproductive season. In one modality, water flow was maintained constant, whereas in another modality, water flow was highly variable. Reproductive investment of all individuals was monitored usingweight and energetic plasma metabolite variation throughout the reproductive season. Only the populations originating fromthe most variable environment showed a plastic response to experimental manipulation of water flow, the females being able to reduce their weight variation (from 19.2% to 13.1%) and metabolites variations (from 84.2% to 18.6% for triglycerides for instance) under variable flow conditions. These results imply that mechanisms to cope with environmental stochasticity can differ between populations of the same species, where some populations can be plastic whereas other cannot

Published
2016

44. Does the long-term dietary replacement of fish meal and fish oil affect the hepatic and intestinal transcriptome of rainbow trout (Oncorhynchus mykiss) juveniles ?

Author

LAZZAROTTO, Viviana, Corraze, Geneviève, Médale, Françoise, ProdInra, Archive Ouverte, Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
fish, endocrine system, oncorhynchus mykiss, animal structures, plant-based diets, urogenital system, alimentation animale, animal diseases, digestive system, rainbow trout, transcriptomics, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, poisson, alimentation végétale, animal nutrition, animal feeding, nutrition animale, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, metabolism, transcriptome, métabolisme
Abstract

Résumé; Does the long-term dietary replacement of fish meal and fish oil affect the hepatic and intestinal transcriptome of rainbow trout (Oncorhynchus mykiss) juveniles ?. Aquaculture 2015: Cutting edge science in Aquaculture

Published
2016

45. Des Pyrénées aux Alpes, la sélection sexuelle dans tous ses états chez la truite commune

Author

Zoé Gauthey, Jordi Gil, Marine Freychet, Laura Royer, Elie Chantriaux, Cédric Tentelier, Arnaud Caudron, Jean Guillard, Arturo Elosegi, Aurélie Manicki, Stéphane Panserat, Olivier Lepais, Alexandre Herman, Jacques Rives, Jean-Christophe Aymes, Stéphane Glise, Hendry, A., Margaret Lang, Jacques Labonne, Ecologie Comportementale et Biologie des Populations de Poissons (ECOBIOP), Institut National de la Recherche Agronomique (INRA)-Université de Pau et des Pays de l'Adour (UPPA), Centre Alpin de Recherche sur les Réseaux Trophiques et Ecosystèmes Limniques (CARRTEL), Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry])-Institut National de la Recherche Agronomique (INRA), Science-Management Interface for Biodiversity Conservation (SCIMABIO Interface), Faculty of Science and Technology, University of the Basque Country, Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Redpath Museum and Department of Biology, Université McGill, Environmental Resources Engineering, Humboldt State University (HSU), Institut National de la Recherche Agronomique (INRA)-Université Savoie Mont Blanc (USMB [Université de Savoie] [Université de Chambéry]), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), McGill University = Université McGill [Montréal, Canada], and ProdInra, Archive Ouverte

Subjects
[SDE.BE] Environmental Sciences/Biodiversity and Ecology, fish, reproduction, salmonidae, sea trout, sélection sexuelle, poisson, salmonids, pyrénées, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, salmo trutta, alpes, ComputingMilieux_MISCELLANEOUS
Abstract

National audience

Published
2015

46. Utilisation of food industry by-products in senegalese sole (Solea senagalensis) diets : effects on growth performance, feed utilisation and body composition

Author

Goitia, I., Arantzamendi, L., San Martin, D., Kaushik, Sadasivam, Corraze, Geneviève, Marine Research Unit, AZTI Tecnalia, Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), European Aquaculture Society (EAS). BEL., Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), and ProdInra, Archive Ouverte

Subjects
fish, body composition, solea senegalensis, [SDV.BA] Life Sciences [q-bio]/Animal biology, alimentation animale, composition corporelle, [SDV.BA]Life Sciences [q-bio]/Animal biology, performance de croissance, coproduit, utilisation des nutriments, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, poisson, animal feeding, sole, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

résumé; Utilisation of food industry by-products in senegalese sole (Solea senagalensis) diets : effects on growth performance, feed utilisation and body composition. Aquaculture Europe 2015

Published
2015

47. Effect of an acute handling stress on hepatic oxidative status of European sea bass fed diets differing in lipid source and carbohydrate content

Author

Castro, Carolina, Perez-Jimenez, Amalia, Coutinho, Filipe, Diogenes, Alexandre, Guerreiro, Inès, Corraze, Geneviève, Panserat, Stéphane, Peres, Helena, Oliva-Teles, Aires, Faculty of Sciences, Lebanese University [Beirut], Centro Interdisciplinar de Investigaçao Marinha e Ambiental (CIIMA), Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), This work was partially supported by the FCT (Foundation for Science and Technology), Portugal (project PTDC/MAR-BIO/4107/2012) and co-financed by the European Regional Development Fund (ERDF) through the COMPETE -Operational Competitiveness Programme and national funds through FCT-, under the project 'PEst-C/MAR/LA0015/2011'. C.C., A.P-J., F.C. and I.G. were supported by grants (SFRH/BD/76297/2011, SFRH/BPD/64684/2009, SFRH/BD/86799/2012, SFRH/BD/76139/2011 respectively) from FCT. A.D. supported by the National Counsel of Technological and Scientific Development (CNPq), Brazil., ProdInra, Archive Ouverte, Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), Lebanese University [Beirut] (LU), and European Aquaculture Society (EAS). BEL.

Subjects
fish, [SDV.BA] Life Sciences [q-bio]/Animal biology, alimentation animale, [SDV.BA]Life Sciences [q-bio]/Animal biology, stress oxydatif, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, stress, poisson, lipid, carbohydrate, oxidative stress, animal feeding, stress d'élevage, sense organs, dicentrarchus labrax, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, lipide
Abstract

Résumé; Effect of an acute handling stress on hepatic oxidative status of European sea bass fed diets differing in lipid source and carbohydrate content. Aquaculture Europe 2015

Published
2015

48. Altered muscle catabolic capacities in juvenile rainbow trout depending of the levels of vitamin intake at first feeding

Author

Panserat, Stephane, Marandel, Lucie, Geurden, Inge, Plagnes Juan, Elisabeth, Pegourie, Gaia, Santigosa, E., Verlhac, V., Nutrition, Aquaculture et Génomique (NUAGE), Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Research Centre for Aimal Nutrition and Health, DSM Nutritional Products France, Research Centre for Animal Nutrition and Health, This work was support by a research project (2013-2015) between INRA and DSM 'Nutritional programming in fish : effects of early intake of vitamins on growth and metabolism in rainbow trout juveniles' coordinated by SP and VV, European Aquaculture Society (EAS). BEL., Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), and ProdInra, Archive Ouverte

Subjects
fish, endocrine system, oncorhynchus mykiss, animal structures, [SDV.BA] Life Sciences [q-bio]/Animal biology, urogenital system, muscle, alimentation animale, animal diseases, [SDV.BA]Life Sciences [q-bio]/Animal biology, programmation nutritionnelle, digestive system, rainbow trout, alimentation précoce, [SDV.AEN] Life Sciences [q-bio]/Food and Nutrition, juvénile, poisson, animal feeding, vitamine, métabolisme, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Résumé; Altered muscle catabolic capacities in juvenile rainbow trout depending of the levels of vitamin intake at first feeding. Aquaculture Europe 2015

Published
2015

49. Eating for two: Consequences of parental nutrition on offspring autophagy

Author

Velez, Emilio José, Marandel, Lucie, Panserat, Stephane, Dias, Karine, Gao, Yujie, Plagnes- Juan, Elisabeth, Geurden, Inge, Skiba, Sandrine, Seiliez, Iban, Nutrition, Aquaculture et Génomique (NUAGE), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université Sciences et Technologies - Bordeaux 1-Institut National de la Recherche Agronomique (INRA), UE, and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
fish, autophagy, épigénétique, poisson, nutrition parentale, Alimentation et Nutrition, méthionine, Food and Nutrition, methionine, epigenetic, autophagie, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition
Abstract

Résumé; Pregestational nutrition events may result in permanent changes in the growth potential, health and metabolic status of the next generation. Possible biological mechanisms for ‘imprinting’ the nutritional event in the next generation comprise epigenetic phenomenon, such as DNA and histone methylation. In the present study, we demonstrate that feeding broodstock fish with a diet deficient in methionine (one of the main methyl-group donor needed for all biological methylation reactions including DNA and histones methylation) strongly perturbs the nutritional control of the expression of several autophagy-related genes in the next generation. Our current research aim at defining the epigenetic mechanisms involved in this effect.

Published
2015

50. Rôle du miR122 dans la régulation nutritionnelle du métabolisme hépatique chez la truite arc-en-ciel

Author

Skiba, Sandrine, Nutrition, Aquaculture et Génomique (NUAGE), and Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)

Subjects
Animal biology, trout, rainbow, [SDV.BA]Life Sciences [q-bio]/Animal biology, Biologie animale, micro arn, métabolisme hépatique, microRNAs, truite arc en ciel, régulation nutritionnelle
Abstract

Rôle du miR122 dans la régulation nutritionnelle du métabolisme hépatique chez la truite arc-en-ciel. Le monde des microARNs

Published
2015

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