1. Noncovalently Functionalized Monolayer Graphene for Sensitivity Enhancement of Surface Plasmon Resonance Immunosensors
- Author
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Michael Holzinger, Meenakshi Singh, Serge Cosnier, Maryam Tabrizian, Nina C. Berner, Sinéad Winters, Georg S. Duesberg, Département de Chimie Moléculaire - Biosystèmes Electrochimiques et Analytiques (DCM - BEA), Département de Chimie Moléculaire (DCM), Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Department of Biomedical Engineering [Montréal] (BME), and McGill University = Université McGill [Montréal, Canada]
- Subjects
Cholera Toxin ,Nanotechnology ,Chemical vapor deposition ,Polypyrrole ,Biochemistry ,Catalysis ,law.invention ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Limit of Detection ,law ,Animals ,[CHIM]Chemical Sciences ,Biotinylation ,Surface plasmon resonance ,ComputingMilieux_MISCELLANEOUS ,Detection limit ,chemistry.chemical_classification ,Graphene ,Nitrilotriacetic acid ,General Chemistry ,Polymer ,Surface Plasmon Resonance ,chemistry ,Graphite ,Gold ,Volatilization ,Antibodies, Immobilized ,Layer (electronics) - Abstract
A highly efficient surface plasmon resonance (SPR) immunosensor is described using a functionalized single graphene layer on a thin gold film. The aim of this approach was two-fold: first, to amplify the SPR signal by growing graphene through chemical vapor deposition and, second, to control the immobilization of biotinylated cholera toxin antigen on copper coordinated nitrilotriacetic acid (NTA) using graphene as an ultrathin layer. The NTA groups were attached to graphene via pyrene derivatives implying π-π interactions. With this setup, an immunosensor for the specific antibody anticholera toxin with a detection limit of 4 pg mL(-1) was obtained. In parallel, NTA polypyrrole films of different thicknesses were electrogenerated on the gold sensing platform where the optimal electropolymerization conditions were determined. For this optimized polypyrrole-NTA setup, the simple presence of a graphene layer between the gold and polymer film led to a significant increase of the SPR signal.
- Published
- 2015