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2 results on '"Narjes MTIMET"'

1. Quantifying the impact of sub-optimal temperature and pH on the variability of germination and growth recovery among sub-populations of heat-stressed Bacillus weihenstephanensis KBAB4 and Bacillus licheniformis Ad978 spores

Author

Coroller, Louis, Trunet, Clément, Mathot, Anne Gabrielle, Narjes MTIMET, Postollec, Florence, Leguerinel, Ivan, Sohier, Danièle, Couvert, Olivier, Carlin, Frederic, Université de Bretagne Occidentale, Association pour le Développement de la Recherche Appliquée aux Industries Agricoles et Alimentaires, Sécurité et Qualité des Produits d'Origine Végétale (SQPOV), Avignon Université (AU)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and ProdInra, Migration

Subjects
[SDV] Life Sciences [q-bio], [SPI.GPROC] Engineering Sciences [physics]/Chemical and Process Engineering, [SDV]Life Sciences [q-bio], [SDV.IDA]Life Sciences [q-bio]/Food engineering, [SPI.GPROC]Engineering Sciences [physics]/Chemical and Process Engineering, [SDV.IDA] Life Sciences [q-bio]/Food engineering, ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
2016

2. Metabolic phenotypes of Saccharomyces cerevisiae mutants with altered trehalose 6-phosphate dynamics

Author

Carlos Gancedo, Marie-Odile Loret, Narjes Mtimet, Ceren Alkim, Azmat Ullah, Jean Marie François, Amélie Vax, Thomas Walther, Gertien J. Smits, Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Fac Sci, Swammerdam Inst Life Sci, University of Amsterdam [Amsterdam] (UvA), Inst Invest Biomed Alberto Sols, Spanish National Research Council (CSIC), ANR (Agence Nationale de la Recherche) Blanc [05-2-42128], Region Midi Pyrenees (France) [09003813], Spanish Ministry of Science and Innovation (MICINN) [BFU2010-19628-C02-02], HEC (Higher Education Commission) Pakistan, Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), Swammerdam Institute for Life Sciences (SILS), Région Midi-Pyrénées, Ministerio de Ciencia e Innovación (España), Higher Education Commission (Pakistan), Agence Nationale de la Recherche (France), Molecular Biology and Microbial Food Safety (SILS, FNWI), and Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
ATPase, [SDV]Life Sciences [q-bio], Mutant, Yarrowia, PLASMA-MEMBRANE ATPASE, yeast, Biochemistry, AMP Deaminase, GLUCOSE, chemistry.chemical_compound, Adenine nucleotide, ETHANOL PULSES, H+-ATPASE, fermentation, YEAST GLYCOLYSIS, 0303 health sciences, biology, Adenine Nucleotides, 4. Education, AMP deaminase, Hydrogen-Ion Concentration, glycolysis, Recombinant Proteins, Isoenzymes, Proton-Translocating ATPases, Glucosyltransferases, GROWTH, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, DEPENDENT ACTIVATION, Down-Regulation, Fungal Proteins, 03 medical and health sciences, Molecular Biology, trehalose, 030304 developmental biology, MOLECULAR-CLONING, Ethanol, hexokinase, 030306 microbiology, Cell Membrane, Cell Biology, Metabolism, biology.organism_classification, Trehalose, GENE, Inosine, chemistry, Mutation, biology.protein, INTRACELLULAR PH, Sugar Phosphates, metabolic regulation
Abstract

In Saccharomyces cerevisiae, synthesis of T6P (trehalose 6-phosphate) is essential for growth on most fermentable carbon sources. In the present study, the metabolic response to glucose was analysed in mutants with different capacities to accumulate T6P. A mutant carrying a deletion in the T6P synthase encoding gene, TPS1, which had no measurable T6P, exhibited impaired ethanol production, showed diminished plasma membrane H+-ATPase activation, and became rapidly depleted of nearly all adenine nucleotides which were irreversibly converted into inosine. Deletion of the AMP deaminase encoding gene, AMD1, in the tps1 strain prevented inosine formation, but did not rescue energy balance or growth on glucose. Neither the 90%-reduced T6P content observed in a tps1 mutant expressing the Tps1 protein from Yarrowia lipolytica, nor the hyperaccumulation of T6P in the tps2 mutant had significant effects on fermentation rates, growth on fermentable carbon sources or plasma membrane H+-ATPase activation. However, intracellular metabolite dynamics and pH homoeostasis were strongly affected by changes in T6P concentrations. Hyperaccumulation of T6P in the tps2 mutant caused an increase in cytosolic pH and strongly reduced growth rates on non-fermentable carbon sources, emphasizing the crucial role of the trehalose pathway in the regulation of respiratory and fermentative metabolism., This work was supported, in part, by the ANR (Agence Nationale de la Recherche) Blanc [grant number 05-2-42128] and by the Region Midi Pyrénées (France) [grant number 09003813]. C.G. was supported by the Spanish Ministry of Science and Innovation (MICINN) [grant number BFU2010-19628-CO2-02]. A.U. thanks the HEC (Higher Education Commission) Pakistan for funding.

Published
2013
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