1. Highly hydrophilic surfaces from polyglycidol grafts with dual antifouling and specific protein recognition properties
- Author
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Mireille Turmine, Teresa Basinska, Sandrine Lépinay, Mohamed M. Chehimi, Dalila Ben Hassen-Chehimi, Monika Gosecka, Marie-Claude Millot, Benjamin Carbonnier, Stanislaw Slomkowski, Sarra Gam-Derouich, Ali Othmane, Interfaces, Traitements, Organisation et Dynamique des Systèmes (ITODYS (UMR_7086)), Université Paris Diderot - Paris 7 (UPD7)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Center of Molecular and Macromolecular Studies, Polska Akademia Nauk = Polish Academy of Sciences (PAN), Institut de Chimie et des Matériaux Paris-Est (ICMPE), Institut de Chimie du CNRS (INC)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Interfaces et Systèmes Electrochimiques (LISE), Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de recherches sur les polymères (LRP), Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Biophysique [Monastir], Faculté de Médecine de Monastir [Tunisie], Laboratoire d'Application de la Chimie aux Ressources et Substances Naturelles et à l'Environnement, Faculté des Sciences de Bizerte [Université de Carthage], Université de Carthage - University of Carthage-Université de Carthage - University of Carthage, Centre National de la Recherche Scientifique (CNRS)-Université Paris Diderot - Paris 7 (UPD7), Polska Akademia Nauk (PAN), Laboratoire de biophysique, and Faculté des Sciences de Bizerte
- Subjects
ADSORPTION ,Biofouling ,Surface Properties ,TRANSFER RADICAL POLYMERIZATION ,02 engineering and technology ,(Hydroxyethyl)methacrylate ,010402 general chemistry ,01 natural sciences ,AQUEOUS-SOLUTION ,GAMMA-GLOBULINS ,Contact angle ,chemistry.chemical_compound ,Adsorption ,SELF-ASSEMBLED MONOLAYERS ,Polymer chemistry ,Electrochemistry ,Animals ,General Materials Science ,Particle Size ,Bovine serum albumin ,Surface plasmon resonance ,BOVINE SERUM-ALBUMIN ,Spectroscopy ,GRADIENT APPROXIMATION FUNCTIONALS ,Aqueous solution ,Molecular Structure ,biology ,EXACT-EXCHANGE ,Chemistry ,DENSITY-FUNCTIONAL THERMOCHEMISTRY ,Serum Albumin, Bovine ,Self-assembled monolayer ,Surfaces and Interfaces ,Stainless Steel ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,DIAZONIUM SALTS ,0104 chemical sciences ,Photopolymer ,Propylene Glycols ,biology.protein ,Cattle ,Gold ,0210 nano-technology ,[CHIM.OTHE]Chemical Sciences/Other - Abstract
International audience; Homopolymer grafts from α-tert-butoxy-ω-vinylbenzyl-polyglycidol (PGL) were prepared on gold and stainless steel (SS) substrates modified by 4-benzoyl-phenyl (BP) moieties derived from the electroreduction of the parent salt 4-benzoyl benzene diazonium tetrafluoroborate. The grafted BP aryl groups efficiently served to surface-initiate photopolymerization (SIPP) of PGL. In similar conditions, SIPP of hydroxyethyl methacrylate (HEMA) permitted the production of PHEMA grafts as model surfaces. Water contact angles were found to be 66°, 15°, and 0° for SS-BP, SS-PHEMA, and SS-PPGL, respectively. The spontaneous spreading of water drops on SS-PPGL was invariably observed with 1.5 μL water drops. PPGL thus appears as a superhydrophilic polymer. Resistance to nonspecific adsorption of proteins of PPGL and PHEMA grafts on gold was evaluated by surface plasmon resonance (SPR) using antibovine serum albumin (anti-BSA). The results conclusively show that PPGL-grafts exhibit enhanced resistance to anti-BSA adsorption compared to the well-known hydrophilic PHEMA. PPGL grafts were further modified with BSA through the carbonyldiimidazole activation of the OH groups providing immunosensing surfaces. The so-prepared PPGL-grafted BSA hybrids specifically interacted with anti-BSA in PBS as compared to antimyoglobin. It is clear that the superhydrophilic character of PPGL grafts opens new avenues for biomedical applications where surfaces with dual functionality, namely, specific protein grafting together with resistance to biofouling, are required.
- Published
- 2011
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