Search

Your search keyword '"Mathilde Triquigneaux"' showing total 6 results
Start Over Author "Mathilde Triquigneaux" Publisher hal ccsd
6 results on '"Mathilde Triquigneaux"'

1. MT9, a natural peptide from black mamba venom antagonizes the muscarinic type 2 receptor and reverses the M2R-agonist-induced relaxation in rat and human arteries

Author

Justyna Ciolek, Claude Zoukimian, Justine Dhot, Mélanie Burban, Mathilde Triquigneaux, Benjamin Lauzier, Christelle Guimbert, Didier Boturyn, Marine Ferron, Lidia Ciccone, Livia Tepshi, Enrico Stura, Pierre Legrand, Philippe Robin, Gilles Mourier, Béatrice Schaack, Imen Fellah, Guillaume Blanchet, Chantal Gauthier-Erfanian, Rémy Beroud, Denis Servent, Michel De Waard, Nicolas Gilles, Service d'Ingénierie Moléculaire pour la Santé (ex SIMOPRO) (SIMoS), Médicaments et Technologies pour la Santé (MTS), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), unité de recherche de l'institut du thorax UMR1087 UMR6291 (ITX), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Nantes Université - UFR de Médecine et des Techniques Médicales (Nantes Univ - UFR MEDECINE), Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ)-Nantes Université - pôle Santé, Nantes Université (Nantes Univ)-Nantes Université (Nantes Univ), Synchrotron SOLEIL (SSOLEIL), Centre National de la Recherche Scientifique (CNRS), Translational microbial Evolution and Engineering (TIMC-TrEE), Translational Innovation in Medicine and Complexity / Recherche Translationnelle et Innovation en Médecine et Complexité - UMR 5525 (TIMC ), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA)-Institut polytechnique de Grenoble - Grenoble Institute of Technology (Grenoble INP ), Université Grenoble Alpes (UGA), Département de Chimie Moléculaire (DCM), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA), Thérapeutique Recombinante Expérimentale (TIMC-IMAG-TheREx ), and Techniques de l'Ingénierie Médicale et de la Complexité - Informatique, Mathématiques et Applications Grenoble - UMR 5525 (TIMC-IMAG)

Subjects
Elapid Venoms, Pharmacology, Bioactive peptide mass spectrometry, Snake venom, Dendroaspis, M2 muscarinic receptor, Cholinergic Agents, Arteries, General Medicine, Receptors, Muscarinic, Rats, Peptide synthesis, Drug screening, [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system, Peptide folding, [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology, Animals, Humans, Peptides, Toxins, Biological, Arterial pressure
Abstract

International audience; All five muscarinic receptors have important physiological roles. The endothelial M2 and M3 subtypes regulate arterial tone through direct coupling to Gq or Gi/o proteins. Yet, we lack selective pharmacological drugs to assess the respective contribution of muscarinic receptors to a given function. We used mamba snake venoms to identify a selective M2R ligand to investigate its contribution to arterial contractions. Using a bio-guided screening binding assay, we isolated MT9 from the black mamba venom, a three-finger toxin active on the M2R subtype. After sequencing and chemical synthesis of MT9, we characterized its structure by X-ray diffraction and determined its pharmacological characteristics by binding assays, functional tests, and ex vivo experiments on rat and human arteries. Although MT9 belongs to the three-finger fold toxins family, it is phylogenetically apart from the previously discovered muscarinic toxins, suggesting that two groups of peptides evolved independently and in a convergent way to target muscarinic receptors. The affinity of MT9 for the M2R is 100 times stronger than that for the four other muscarinic receptors. It also antagonizes the M2R/G i pathways in cell-based assays. MT9 acts as a non-competitive antagonist against acetylcholine or arecaine, with low nM potency, for the activation of isolated rat mesenteric arteries. These results were confirmed on human internal mammary arteries. In conclusion, MT9 is the first fully characterized M2R-specific natural toxin. It should provide a tool for further understanding of the effect of M2R in various arteries and may position itself as a new drug candidate in cardiovascular diseases

Published
2022

2. Use of X-band EPR to follow the reaction between various nucleophilic compounds and 2-methyl-2-nitrosopropane (MNP)

Author

Béatrice Tuccio, Mathilde Triquigneaux, Laurence Charles, Laboratoire Chimie Provence (LCP), Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC), Institut de Chimie Radicalaire (ICR), Aix Marseille Université (AMU)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Nitroxide mediated radical polymerization, Stereochemistry, General Chemical Engineering, 010401 analytical chemistry, General Engineering, 2-Methyl-2-nitrosopropane, Nitroso, 010402 general chemistry, 01 natural sciences, Medicinal chemistry, 0104 chemical sciences, Analytical Chemistry, law.invention, Adduct, chemistry.chemical_compound, chemistry, Nucleophile, law, Moiety, [CHIM]Chemical Sciences, Reactivity (chemistry), Electron paramagnetic resonance
Abstract

International audience; Continuous wave X-band EPR spectroscopy was used as an analytical tool to follow the formation of nitroxide radicals after the reaction between various nucleophiles and 2-methyl-2-nitrosopropane (MNP). The addition of amines or of trihalogeno-acetic acids onto the nitroso moiety was found to depend on experimental parameters such as exposure to white light or the nature of the solvent, while no nitroxide adducts were ever detected when alcohols or weak carboxylic acids were used. In contrast to results previously obtained with cysteine, the reaction of ten standard amino acids (Gly, Lys, Arg, His, Trp, Ser, Thr, Tyr, Asp and Glu) on MNP never led to an EPR observable adduct, further confirming the particular reactivity of this nitroso compound towards thiols.

Published
2011

3. Ene-reaction between a dienolic compound and 2-methyl-2-nitrosopropane: an EPR-MS study

Author

Mathilde Triquigneaux, Christiane André-Barrès, Laurence Charles, Béatrice Tuccio, Laboratoire Chimie Provence (LCP), Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC), Aix Marseille Université (AMU), and Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0303 health sciences, Nitroxide mediated radical polymerization, 010405 organic chemistry, 030302 biochemistry & molecular biology, Organic Chemistry, 2-Methyl-2-nitrosopropane, Photochemistry, Mass spectrometry, 01 natural sciences, Biochemistry, 0104 chemical sciences, law.invention, 03 medical and health sciences, chemistry.chemical_compound, Hydroxylamine, chemistry, law, Drug Discovery, Organic chemistry, [CHIM]Chemical Sciences, Electron paramagnetic resonance, Ene reaction
Abstract

International audience; The combination of electron paramagnetic resonance (EPR) and mass spectrometry (MS) was used as an efficient tool to elucidate the mechanism of an ene-reaction between a dienol compound and 2-methyl-2-nitrosopropane. (C) 2010 Elsevier Ltd. All rights reserved.

Published
2010

4. Combining EPR and ESI-MS/MS to study the reactivity of alkylthiols and cysteine towards 2-methyl-2-nitrosopropane (MNP)

Author

Béatrice Tuccio, Mathilde Triquigneaux, Laurence Charles, Aix Marseille Université (AMU), Laboratoire Chimie Provence (LCP), Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), and Centre National de la Recherche Scientifique (CNRS)-Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC)

Subjects
Nitroxide mediated radical polymerization, Spin trapping, Chemistry, General Chemical Engineering, Electrospray ionization, 010401 analytical chemistry, General Engineering, 2-Methyl-2-nitrosopropane, 010402 general chemistry, Tandem mass spectrometry, Photochemistry, Mass spectrometry, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, Adduct, chemistry.chemical_compound, Moiety, Organic chemistry, [CHIM]Chemical Sciences
Abstract

International audience; An approach combining EPR spectroscopy and electrospray ionisation mass spectrometry was employed to study the reactivity of two alkylthiols and of cysteine towards 2-methyl-2-narosopropane (MNP) in the presence of H(2)O(2) Depending on the experimental conditions, various nitroxides could be EPR-detected, while their diamagnetic derivatives were structurally characterised after tandem mass spectrometry experiments Sample dilution in methanol before electrospray ionisation was also found to generate methyl-hydroxylamine derivatives and could thus constitute a simple derivatisation process to stabilise hardly MS-detectable radical species Upon white light irradiation, the three thiols tested led to alkoxynitroxides according to an inverted spin trapping process On the other hand, only the reduced form of cysteine was found to react with MNP in the dark, yielding an alkylthionitroxide adduct via a Forrester-Hepburn mechanism Besides a better understanding of the reactivity of thiols towards nitroso compounds, the results obtained underlined the particular behaviour of cysteine, suggesting that the use of a molecule as simple as MNP could allow a nitroxide moiety to be linked to a cysteinyl residue

Published
2010

5. A combined spin trapping/EPR/mass spectrometry approach to study the formation of a cyclic peroxide by dienolic precursor autoxidation

Author

Laurence Charles, Mathilde Triquigneaux, Christiane André-Barrès, Béatrice Tuccio, Laboratoire Chimie Provence (LCP), Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique (SPCMIB), Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Electrospray, Pyridines, Organophosphonates, Analytical chemistry, Alkenes, 010402 general chemistry, Photochemistry, Mass spectrometry, Tandem mass spectrometry, 01 natural sciences, Biochemistry, Peroxide, Mass Spectrometry, law.invention, Adduct, chemistry.chemical_compound, law, [CHIM]Chemical Sciences, Physical and Theoretical Chemistry, Electron paramagnetic resonance, Autoxidation, Spin trapping, 010405 organic chemistry, Organic Chemistry, Electron Spin Resonance Spectroscopy, Peroxides, 0104 chemical sciences, Oxygen, chemistry, Oxidation-Reduction
Abstract

International audience; The spontaneous addition of air oxygen to a dienolic compound, yielding a cyclic peroxide, was followed by spin trapping (ST) combined with EPR spectroscopy and mass spectrometry (MS). Using two different nitrones, the ST/EPR study allowed the detection of the spin adduct of a radical intermediate, and the radical centre in the addend was identified after similar experiments performed with two different (13)C-labelled analogues of the substrate. The media were also submitted to electrospray ionisation, in both positive and negative modes, for structural characterisation of the spin adducts by tandem mass spectrometry. This allowed the structure of the hydroxylamine derivatives of the nitroxides formed to be identified. Following these results, a mechanism pathway was proposed for this autoxidation.

Published
2010

6. 5-Hydroxy-2,2,6,6-tetramethyl-4-(2-methylprop-1-en-yl)cyclohex-4-ene-1,3 -dione, a novel cheletropic trap for nitric oxide EPR detection

Author

Béatrice Tuccio, Laurence Charles, Robert Lauricella, Mathilde Triquigneaux, Christiane André-Barrès, Chimie, biologie et radicaux libres - UMR 6517 (CBRL), Université de la Méditerranée - Aix-Marseille 2-Université Paul Cézanne - Aix-Marseille 3-Université de Provence - Aix-Marseille 1-Centre National de la Recherche Scientifique (CNRS), Synthèse et Physico-Chimie de Molécules d'Intérêt Biologique (SPCMIB), Institut de Chimie de Toulouse (ICT-FR 2599), Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Laboratoire Chimie Provence (LCP), Université de Provence - Aix-Marseille 1-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS), Aix Marseille Université (AMU), Institut de Chimie de Toulouse (ICT), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), and Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Nitroxide mediated radical polymerization, Radical, chemistry.chemical_element, Nitric Oxide, 010402 general chemistry, Photochemistry, 01 natural sciences, Oxygen, Catalysis, Nitric oxide, law.invention, Trap (computing), chemistry.chemical_compound, law, Materials Chemistry, [CHIM]Chemical Sciences, Electron paramagnetic resonance, Ene reaction, Cyclohexanones, 010405 organic chemistry, Electron Spin Resonance Spectroscopy, Metals and Alloys, General Chemistry, 0104 chemical sciences, 3. Good health, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, chemistry, Ceramics and Composites, Oxidation-Reduction
Abstract

International audience; The title compound behaves as an efficient cheletropic trap for both NO and NO(2) radicals in the presence of oxygen, yielding EPR observable nitroxide and alkoxynitroxide, respectively.

Published
2010

Catalog

Books, media, physical & digital resources
See catalog results