1. Seroreactivity against Specific L5P Antigen from Mycobacterium avium subsp. paratuberculosis in Children at Risk for T1D
- Author
-
Leonardo Antonio Sechi, Magdalena Niegowska, Maria Luisa Manca Bitti, Simona Piccinini, Frank Biet, Novella Rapini, Roberta Lidano, Sylvie Bay, Department of Biomedical Sciences, University of Sassari, Pediatric Diabetology Unit, Policlinico di Tor Vergata, Università degli Studi di Roma Tor Vergata [Roma], UR Infectiologie animale et Santé publique (UR IASP), Institut National de la Recherche Agronomique (INRA), Chimie des Biomolécules, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Università degli Studi di Sassari = University of Sassari [Sassari] (UNISS), Infectiologie et Santé Publique (UMR ISP), Institut National de la Recherche Agronomique (INRA)-Université de Tours (UT), Chimie des Biomolécules - Chemistry of Biomolecules, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Seyed Ehtesham Hasnain, Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut National de la Recherche Agronomique (INRA)-Université de Tours, and Sechi, Leonardo A
- Subjects
0301 basic medicine ,Proteomics ,Paratuberculosis ,lcsh:Medicine ,Biochemistry ,Animal Diseases ,0403 veterinary science ,Families ,Endocrinology ,Medicine and Health Sciences ,Enzyme-Linked Immunoassays ,lcsh:Science ,enfant ,Immune Response ,Children ,Multidisciplinary ,biology ,Microbiology and Parasitology ,04 agricultural and veterinary sciences ,Microbiologie et Parasitologie ,Mycobacterium avium subsp. paratuberculosis ,[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology ,Zinc Transporter 8 ,Antibody ,Research Article ,diabète de type 1 ,040301 veterinary sciences ,Endocrine Disorders ,Immunology ,Médecine humaine et pathologie ,Research and Analysis Methods ,Genetic Predisposition ,Peptide Mapping ,03 medical and health sciences ,Immune system ,Antigen ,medicine ,Genetic predisposition ,Diabetes Mellitus ,Genetics ,Immunoassays ,Molecular Biology Techniques ,Molecular Biology ,technique elisa ,Gene Mapping ,lcsh:R ,Biology and Life Sciences ,medicine.disease ,biology.organism_classification ,Virology ,030104 developmental biology ,Epitope mapping ,Age Groups ,Metabolic Disorders ,People and Places ,Genetics of Disease ,biology.protein ,Immunologic Techniques ,Human health and pathology ,Population Groupings ,lcsh:Q ,Zoology ,Epitope Mapping ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology ,Mycobacterium - Abstract
erratum : doi :10.1371/journal.pone.0161516 wos : 000381476700072; Although numerous environmental agents have been investigated over the years as possible triggers of type 1 diabetes (T1D), its causes remain unclear. We have already demonstrated an increased prevalence of antibodies against peptides derived from Mycobacterium avuim subsp. paratuberculosis (MAP) homologous to human zinc transporter 8 protein (ZnT8) and proinsulin in Italian subjects at risk for or affected by T1D. In this study, we compared titers of the previously detected antibodies with seroreactivity to MAP lipopentapetide (L5P) that recently emerged as a strong immunogenic component able to specifically distinguish MAP from other mycobacteria.[br/]Plasma of 32 children and youth at risk for T1D including follow-up samples and 42 age-matched healthy controls (HC) recruited at the Tor Vergata University Hospital in Rome was analyzed by indirect ELISA for the presence of antibodies against MAP-derived epitopes MAP3865c133-141, MAP3865c125-133, MAP2404c70-85 and MAP1,4αgbp157-173 along with their ZnT8 and proinsulin homologs. The data were analyzed through two-tailed Mann-Whitney U test and relation between variables was determined by principal component analysis.[br/]Responses to L5P were not detectable in subjects whose initial seroreactivity to MAP peptides and their human homologs was lost in follow-up samples, whereas anti-L5P antibodies appeared constantly in individuals with a stable immunity against MAP antigens. The overall coincidence in positivity to L5P and the four MAP epitopes both in children at risk for T1D and HC exceeded 90%.[br/]MAP-derived homologs may cross-react with ZnT8 and proinsulin peptides inducing immune responses at a young age in subjects predisposed for T1D. Thus, L5P may have a diagnostic value to immediately indicate the presence of anti-MAP seroreactivity when evaluation of a more complex antibody status is not required. Almost complete coincidence in responses to both types of antigens lends support to the involvement of MAP in T1D.
- Published
- 2016
- Full Text
- View/download PDF