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2 results on '"Magalie Vatin"'

1. Nitrogen fixation in a chemoautotrophic lucinid symbiosis

Author

Magalie Vatin, Didier Mbéguié-A-Mbéguié, Andrea Thürmer, Jennifer Tocny, Tjorven Hinzke, Olivier Gros, Stephanie Markert, Ruby Ponnudurai, Rolf Daniel, Anja Poehlein, Thomas Schweder, Stefan E. Heiden, Susann Meyer, Sten König, Dörte Becher, Biologie de la Mangrove (BM), Evolution Paris Seine, Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), EU [264774], German Science Foundation (DFG) [MA6346/2-1], French National Program EC2CO Biohefect/Ecodyn/Dril/MicrobiEn `Fixation de l'azote chez les bivalves marins de la famille des Lucinidae: impact sur le fonctionnement de ces organismes et des ecosystemes tropicaux cotiers associes', Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles et de la Guyane (UAG)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Nice Sophia Antipolis (1965 - 2019) (UNS)

Subjects
Gills, 0301 basic medicine, Microbiology (medical), Chemoautotrophic Growth, animal structures, Proteome, 030106 microbiology, Immunology, [SDV.BID]Life Sciences [q-bio]/Biodiversity, Biology, Applied Microbiology and Biotechnology, Microbiology, 03 medical and health sciences, Symbiosis, Nitrogen Fixation, Nitrogenase, Botany, Gammaproteobacteria, Genetics, Animals, 14. Life underwater, Phylogeny, Host (biology), fungi, food and beverages, Sequence Analysis, DNA, Cell Biology, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, Bivalvia, 030104 developmental biology, M40 - Écologie aquatique, Nitrogen fixation, bacteria, Proteobacteria, Oxidoreductases, Metabolic Networks and Pathways, Codakia orbicularis
Abstract

The shallow water bivalve Codakia orbicularis lives in symbiotic association with a sulfur-oxidizing bacterium in its gills. The endosymbiont fixes CO2 and thus generates organic carbon compounds, which support the host's growth. To investigate the uncultured symbiont's metabolism and symbiont–host interactions in detail we conducted a proteogenomic analysis of purified bacteria. Unexpectedly, our results reveal a hitherto completely unrecognized feature of the C. orbicularis symbiont's physiology: the symbiont's genome encodes all proteins necessary for biological nitrogen fixation (diazotrophy). Expression of the respective genes under standard ambient conditions was confirmed by proteomics. Nitrogenase activity in the symbiont was also verified by enzyme activity assays. Phylogenetic analysis of the bacterial nitrogenase reductase NifH revealed the symbiont's close relationship to free-living nitrogen-fixing Proteobacteria from the seagrass sediment. The C. orbicularis symbiont, here tentatively named ‘Candidatus Thiodiazotropha endolucinida’, may thus not only sustain the bivalve's carbon demands. C. orbicularis may also benefit from a steady supply of fixed nitrogen from its symbiont—a scenario that is unprecedented in comparable chemoautotrophic symbioses.

Published
2016

2. Refined Mapping of a Quantitative Trait Locus on Chromosome 1 Responsible for Mouse Embryonic Death

Author

Catherine Serres, Magalie Vatin, Paul Laissue, Daniel Vaiman, Virginie Firlej, Gilles Renault, Françoise Mondon, Xavier Montagutelli, Gaetan Burgio, Ahmed Ziyyat, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Génétique des Mammifères, Institut Pasteur [Paris] (IP), University of Tasmania [Hobart, Australia] (UTAS), Universidad del Rosario [Bogota], Génétique fonctionnelle de la Souris, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), MV is a PhD student funded by the French Ministry of Research (Doctoral School Gc2ID, Université Paris-Descartes)., We thank I. Lanctin and J. Chevalier (Pasteur Institute, Paris) for the IRCS breeding and C. Marchiol (Small animal imaging facility of the Cochin Institute, Paris) for their technical assistance. We thank J. Coquet for critical reading of the manuscript., Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Pasteur [Paris], and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)

Subjects
Heredity, MESH: Chromosomes, Mammalian, Gene Expression, Genetic analysis, Gene, MESH: Genotype, Mice, 0302 clinical medicine, MESH: Pregnancy, Pregnancy, MESH: Reverse Transcriptase Polymerase Chain Reaction, Genotype, MESH: Animals, MESH: Embryo Loss, Genetics, 0303 health sciences, education.field_of_study, 030219 obstetrics & reproductive medicine, Multidisciplinary, Reverse Transcriptase Polymerase Chain Reaction, Cell-proliferation, Obstetrics and Gynecology, Genomics, Cytogenetic analysis, Trait Locus, Phenotype, Ginecología, Embryo Loss, Medicine, Female, Research Article, Embarazo, Science, Population, Quantitative Trait Loci, Congenic, Quantitative trait locus, Biology, Recurrent miscarriage, 03 medical and health sciences, Genome Analysis Tools, Animals, education, Trait Locus Analysis, MESH: Mice, 030304 developmental biology, Spontaneous abortion, [SDV.GEN]Life Sciences [q-bio]/Genetics, Lethality, Quantitative Traits, Chromosome, Computational Biology, Prefnancies, Ginecología & otras especialidades médicas, Preeclampsia, Chromosomes, Mammalian, MESH: Quantitative Trait Loci, Aborto, Miscarriage and Stillbirth, Disruption, MESH: Microsatellite Repeats, Animal Genetics, MESH: Female, Microsatellite Repeats, Developmental Biology
Abstract

International audience; Recurrent spontaneous abortion (RSA) is defined as the loss of three or more consecutive pregnancies during the first trimester of embryonic intrauterine development. This kind of human infertility is frequent among the general population since it affects 1 to 5% of women. In half of the cases the etiology remains unelucidated. In the present study, we used interspecific recombinant congenic mouse strains (IRCS) in the aim to identify genes responsible for embryonic lethality. Applying a cartographic approach using a genotype/phenotype association, we identified a minimal QTL region, of about 6 Mb on chromosome 1, responsible for a high rate of embryonic death (,30%). Genetic analysis suggests that the observed phenotype is linked to uterine dysfunction. Transcriptomic analysis of the uterine tissue revealed a preferential deregulation of genes of this region compared to the rest of the genome. Some genes from the QTL region are associated with VEGF signaling, mTOR signaling and ubiquitine/proteasome-protein degradation pathways. This work may contribute to elucidate the molecular basis of a multifactorial and complex human disorder as RSA.

Published
2012

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